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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae R6
  • locus tag: spr0727 [new locus tag: SPR_RS03645 ]
  • pan locus tag?: PNEUPAN001806000
  • symbol: glnP
  • pan gene symbol?: artP
  • synonym:
  • product: ABC transporter membrane-spanning permease - glutamine transport

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: spr0727 [new locus tag: SPR_RS03645 ]
  • symbol: glnP
  • product: ABC transporter membrane-spanning permease - glutamine transport
  • replicon: chromosome
  • strand: +
  • coordinates: 726847..727536
  • length: 690
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: AE007317 (726847..727536) NCBI
  • BioCyc: SPR0727 BioCyc
  • MicrobesOnline: 133612 MicrobesOnline
  • PneumoBrowse for strain D39V: SPV_0719 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    GTGACTATGAATTTTTCTTTTTTACCTAAGTATTTACCTTATTTTAACTATGGGGCTGTT
    GTGACGATTCTTATTTCTATCTGTGTTATCTTTTTGGGAACTATTTTGGGTGTTGTCTTG
    GCTTTTGGGCAACGTTCAAAGTTTAAACCGCTTGTTTGGTTGGCCAACTTGTACGTTTGG
    ATTTTCCGTGGGACACCGATGATGGTTCAAATTATGATTGCCTTTGCTCTTATGCATATC
    AATGCTCCGACTATTCAGATTGGAATTTTAGGTGTTGATTTTTCGCGTCTGATTCCAGGG
    ATTTTGATTATCTCTATGAATAGTGGTGCTTATGTTTCGGAGATTGTTCGTGCCGGAATC
    AATGCGGTTCCAAAAGGTCAGCTAGAGGCGGCTTATTCGCTAGGGATTCGTCCTAAAAAT
    GCGATGCGTTATGTGATTTTGCCACAAGCAGTCAAAAATATCTTGCCAGCATTGGGGAAC
    GAATTTATCACCATTATCAAGGACAGCTCCCTCTTATCAGCTATTGGGGTCATGGAGTTG
    TGGAATGGGGCTACAACAGTTTCTACAACAACCTATCTACCTTTAACACCACTTTTATTT
    GCAGCATTTTACTACTTGATTATGACCTCTATTCTGACAGTAGCCTTGAAAGCTTTTGAA
    AAACATATGGGACAAGGAGATAAGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    690

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: spr0727 [new locus tag: SPR_RS03645 ]
  • symbol: GlnP
  • description: ABC transporter membrane-spanning permease - glutamine transport
  • length: 229
  • theoretical pI: 10.1212
  • theoretical MW: 25203.2
  • GRAVY: 0.755022

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 137.6)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 130.7)
    and 6 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 68.6)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 28.6)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 19.9)
    2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 15.2)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 13.9)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 12.3)
  • TheSEED  :
    • Arginine ABC transporter, substrate-binding and permease protein
  • ⊞PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 73.8)
    and 1 more
    no clan defined Phlebovirus_G1; Phlebovirus glycoprotein G1 (PF07243; HMM-score: 8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001575
    • TAT(Tat/SPI): 0.000301
    • LIPO(Sec/SPII): 0.006281
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: AAK99531 NCBI
  • UniProt: Q8DQD5 UniProt

⊟Protein sequence[edit | edit source]

  • MTMNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRSKFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILIISMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEFITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFEKHMGQGDKK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: glnP > glnQ

⊟Regulation[edit | edit source]

  • ⊞regulators: AhrC (activation (repression)) regulon, ArgR1* (activation (repression)) regulon
    AhrC(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise 
    ArgR1*(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0719 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=spr0727&oldid=129381"
  • This page was last edited on 20 January 2022, at 18:03.
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