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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 11-FEB-2025

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae ATCC 700669
  • locus tag: SPN23F_RS05150 [old locus tags: SPN23F10100 SPN23F_10100 ]
  • pan locus tag?: PNEUPAN002230000
  • symbol: SPN23F_RS05150
  • pan gene symbol?: —
  • synonym:
  • product: gamma-glutamyl-gamma-aminobutyrate hydrolase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPN23F_RS05150 [old locus tags: SPN23F10100 SPN23F_10100 ]
  • symbol: SPN23F_RS05150
  • product: gamma-glutamyl-gamma-aminobutyrate hydrolase family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 976198..976887
  • length: 690
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_011900 (976198..976887) NCBI
  • BioCyc: SPN23F_RS05150 BioCyc
  • MicrobesOnline: see SPN23F10100
  • PneumoBrowse for strain D39V: SPV_0974 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAAAACCAGTTATTGGGATTACAGGAAACGAAAAAACTCATCCAGATGATGACATC
    ATGATGAGCTACGCAGCAAAAGGCTTTGTTGAAGGCGTTAAAGATGCTGGAGGGATTCCC
    ATCATCCTACCGATTGGTGATCAAGAAATGGCCTGCCACTATATCAGTTTGATTGACAAG
    CTCATCTTGACAGGTGGGCAAAATGTCGATCCAAAATTTTATGGCGAACCCAAAACCATT
    GATAGTGATGACTATCACCTTCAAAGAGATATCTTCGAACTGGCCCTCATCAAGGAAGCT
    ATTAAACAGAAAAAGCCCATTTTCTCTGTCTGCCGTGGTACCCAACTCTTTAACGTTGCC
    ATGGGTGGAACTTTGTACCAAGATATCGAAGACCATTGGCAGGATTCTTCTGTTGAGTAC
    ACAACTCAACGCTTGGTAACAGAACCAGATACCGTTCTCCAAGAAATCTATGGAGAAATC
    TCTCATATCAACTCCTTCCACCACCAGAGCATCAAGGACTTAGCACCAAATTTAAAAGTT
    GTGGCTCATGATCCTAAAGATGGTATCATTGAGGCTGTCATGAGTACGGATGATGTCGCC
    TTTCTCGGTGTCCAATGGCATCCAGAACTTCTTTTTGAAAATCGTCCCAAAGATAAAAAA
    CTCTTTGACTATGTCGTTAATGAACTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    690

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPN23F_RS05150 [old locus tags: SPN23F10100 SPN23F_10100 ]
  • symbol: SPN23F_RS05150
  • description: gamma-glutamyl-gamma-aminobutyrate hydrolase family protein
  • length: 229
  • theoretical pI: 4.69612
  • theoretical MW: 25812.2
  • GRAVY: -0.292576

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 38.4)
    and 5 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 25.5)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 23.2)
    glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (TIGR00566; HMM-score: 21.7)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 13.7)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides intracellular protease, PfpI family (TIGR01382; HMM-score: 12.7)
  • TheSEED: see SPN23F10100
  • ⊞PFAM:
    Glutaminase_I (CL0014) Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 246.1)
    and 2 more
    GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 55.7)
    DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.997
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0027
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009755
    • TAT(Tat/SPI): 0.000252
    • LIPO(Sec/SPII): 0.000535
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000743330 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILPIGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIKQKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEISHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKDKKLFDYVVNEL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SPN23F_RS05150 < SPN23F_RS05155 < SPN23F_RS05160 < SPN23F_RS05165 < SPN23F_RS05170 < SPN23F_RS05175 < SPN23F_RS05180

⊟Regulation[edit | edit source]

  • regulator: Rex see SPN23F10100

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0974 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPN23F_RS05150&oldid=54182"
  • This page was last edited on 20 January 2022, at 15:08.
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