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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 25-NOV-2024

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae R6
  • locus tag: SPR_RS02960 [old locus tag: spr0583 ]
  • pan locus tag?: PNEUPAN001580000
  • symbol: SPR_RS02960
  • pan gene symbol?: cbpL
  • synonym:
  • product: N-acetylmuramoyl-L-alanine amidase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPR_RS02960 [old locus tag: spr0583 ]
  • symbol: SPR_RS02960
  • product: N-acetylmuramoyl-L-alanine amidase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 599026..600015
  • length: 990
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_003098 (599026..600015) NCBI
  • BioCyc: see spr0583
  • MicrobesOnline: see spr0583
  • PneumoBrowse for strain D39V: SPV_0579 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATAAACGTCTATTTTTAAAAATGAGTCTGGTGACGTTGCCAATTTTAGCCTTGTTT
    TCACAACCAGTTTTGGCGGAAGAAAACATCCATTTTTCGAGCTGTAAGGAAGCTTGGGCG
    AATGGCTATTCGGATATTCACGAGGGGGAACCTGGTTATTCTGCCAAGTTAGACCGTGAT
    CATGATGGTGTGGCTTGCGAATTGAAAAATGCTCCTAAGGGTGCTTTTAAAGCAAAACAG
    TCAACGGCTATTCAAATCAACACAAGTTCAGCAACAACAAGTGGTTGGGTTAAGCAGGAC
    GGCGCTTGGTACTACTTTGATGGAAATGGAAATCTAGTGAAAAATGCATGGCAGGGAAGC
    TATTACCTGAAAGCTGATGGTAAAATGGCACAGAGTGAATGGATTTATGACTCTTCTTAT
    CAAGCTTGGTATTATTTGAAATCAGATGGTTCTTATGCAAAAAATGCATGGCAAGGAGCT
    TATTACCTTAAATCAAACGGTAAAATGGCACAAGGTGAGTGGGTTTATGATTCTTCTTAC
    CAAGCATGGTATTACTTGAAATCAGATGGTTCATATGCTCGCAATGCATGGCAAGGAAAC
    TACTATTTGAAATCAGATGGTAAAATGGCTAAAGGTGAATGGGTTTATGATGCCACCTAT
    CAAGCTTGGTATTATTTGACATCAGATGGTTCTTATGCTTACAGTACATGGCAAGGAAAT
    TACTATCTAAAATCGGATGGTAAAATGGCTGTCAATGAATGGGTTGATGGTGGACGTTAT
    TATGTTGGCGCTGACGGAGTTTGGAAGGAAGGTCAAGCAAGTACAGCTTCTTCTAGTAAT
    GATAGCAATAGTGAATATTCTGCTGCTTTAGGAAAGGCAAAAAGTTATAATTCGTTATTC
    CACATGTCAAAAAAACGTATGTATAGACAATTAACTTCTGATTTTGATAAATTTTCAAAT
    GATGCAGCTCAATATGCCATTGATCATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    990

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPR_RS02960 [old locus tag: spr0583 ]
  • symbol: SPR_RS02960
  • description: N-acetylmuramoyl-L-alanine amidase family protein
  • length: 329
  • theoretical pI: 7.56353
  • theoretical MW: 37270.8
  • GRAVY: -0.729483

⊟Function[edit | edit source]

  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 36.6)
  • TheSEED: see spr0583
  • ⊞PFAM:
    Choline_binding (CL0694) Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 125.3)
    and 6 more
    Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 80.5)
    no clan defined Lipoprotein_Ltp; Host cell surface-exposed lipoprotein (PF07553; HMM-score: 63.1)
    Choline_binding (CL0694) Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 60.9)
    no clan defined Excalibur; Excalibur calcium-binding domain (PF05901; HMM-score: 47.8)
    PmbA_TldD_M; PmbA/TldA metallopeptidase central domain (PF19290; HMM-score: 14.1)
    Transthyretin (CL0287) DUF3823; Protein of unknown function (DUF3823) N-terminal domain (PF12866; HMM-score: 9.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • ⊞DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0.0038
    • Extracellular Score: 0.996
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 26 aa
    • SP(Sec/SPI): 0.997363
    • TAT(Tat/SPI): 0.001338
    • LIPO(Sec/SPII): 0.000744
    • Cleavage Site: CS pos: 26-27. VLA-EE. Pr: 0.9904
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001042503 NCBI
  • UniProt: see spr0583

⊟Protein sequence[edit | edit source]

  • MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYSDIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGAWYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGAYYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDATYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTASSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDH

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: no polycistronic organisation predicted

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0579 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Javier Gutiérrez-Fernández, Malek Saleh, Martín Alcorlo, Alejandro Gómez-Mejía, David Pantoja-Uceda, Miguel A Treviño, Franziska Voß, Mohammed R Abdullah, Sergio Galán-Bartual, Jolien Seinen, Pedro A Sánchez-Murcia, Federico Gago, Marta Bruix, Sven Hammerschmidt, Juan A Hermoso
Modular Architecture and Unique Teichoic Acid Recognition Features of Choline-Binding Protein L (CbpL) Contributing to Pneumococcal Pathogenesis.
Sci Rep: 2016, 6;38094
[PubMed:27917891] [WorldCat.org] [DOI] (I e)

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPR_RS02960&oldid=127160"
  • This page was last edited on 20 January 2022, at 17:57.
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