⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 13-FEB-2020

⊟Summary

• organism: Streptococcus pneumoniae D39V
locus tag: SPV_0060 [new locus tag: SPV_RS00305 ]
pan locus tag^?: PNEUPAN000700000
symbol: purK
pan gene symbol^?: purK
• synonym:
• product: N5-carboxyaminoimidazole ribonucleotide synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPV_0060 [new locus tag: SPV_RS00305 ]
• symbol: purK
• product: N5-carboxyaminoimidazole ribonucleotide synthase
• replicon: chromosome
• strand: +
• coordinates: 57784..58875
• length: 1092
• essential: unknown

⊟Accession numbers

• Location: CP027540 (57784..58875) NCBI
• BioCyc: SPV_0060 BioCyc
• MicrobesOnline:
• PneumoBrowse: SPV_0060 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  
  ATGAGCTCATCTAAAACAATCGGAATTATCGGTGGCGGTCAACTGGGTCAGATGATGGCC
  ATTTCTGCTATCTACATGGGCCACAAGGTTATCGCGCTGGATCCTGCGGCGGATTGCCCG
  GCCTCTCGCGTGGCGGAAATCATTGTGGCACCTTATAACGATGTGGACGCCCTCCGTCAG
  TTGGCAGACCGTTGCGATGTCCTCACTTACGAGTTTGAAAATGTCGACGCTGACGGTTTG
  GATGCCGTTATCAAGGATGGACAACTCCCTCAAGGAACAGATCTGCTCCGCATTTCGCAA
  AATCGTATTTTTGAAAAGGACTTTTTGTCAAACAAGTCTCAAGTCACTGTGGCACCCTAC
  AAGGTCGTGACTTCTAGCCTAGACTTGGCAGATATCGACTTGTCGAAAAACTATGTCCTC
  AAGACTGCGACTGGTGGCTACGATGGTCATGGACAAAAGGTTATTCGTTCAGAAGCAGAC
  TTGGAAGCAGCCTATGCGCTAGCAGACTCAGCAGACTGCGTCTTGGAAGAATTTGTCAAC
  TTTGACCTTGAGATTTCTGTCATCGTGTCAGGAAATGGCAAGGAGGTGACGTTTTTCCCA
  GTTCAGGAAAATATCCACCGCAACAATATCCTGTCTAAGACCATCGTACCAGCCCGCATT
  TCTGAAAGTCTAGTAGATAAGGCTAAAGCTATGGCAGTGCGAATCGCAGAACAACTCAAC
  TTGTCTGGAACTCTCTGTGTGGAAATGTTTGCGACTGATGATGACGTTATTGTCAATGAA
  ATCGCCCCACGACCACATAACTCTGGGCACTATTCTATTGAAGCCTGTGATTTCTCTCAG
  TTTGACACCCATATTCTCGGTGTTCTCGGAGCACCATTACCAGTCATCAAACTCCATGCG
  CCAGCCGTTATGCTTAATGTCCTCGGTCAGCATGTCGAGGCTGCTGAGCAATATGTGACA
  GAAAATTCAAGCGCCCACCTCCACATGTATGGTAAAATAGAAGCGAAGCACAACCGCAAG
  ATGGGACATGTGACCTTGTTTAGTGATGTACCGGATAGTGTGGATGAGCTTGGGGAAGGG
  ATTGATTTTTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1092
  

⊟Protein

⊟General

• locus tag: SPV_0060 [new locus tag: SPV_RS00305 ]
• symbol: PurK
• description: N5-carboxyaminoimidazole ribonucleotide synthase
• length: 363
• theoretical pI: 4.51283
• theoretical MW: 39356.4
• GRAVY: 0.00606061

⊟Function

• ⊞⊞reaction:
  EC 6.3.4.18?  ExPASy
  5-(carboxyamino)imidazole ribonucleotide synthase ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO₃(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
• ⊞⊞TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 359.5)
  and 8 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 139.3)
  Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylC (TIGR03909; HMM-score: 32.7)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 27.4)
  Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 23.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 20.6)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 16.9)
  ParB/RepB/Spo0J family partition protein (TIGR00180; HMM-score: 14.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 13.2)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• ⊞⊞PFAM:
  ATP-grasp (CL0179) ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 161.2)
  and 8 more
  Hybrid (CL0105) PurK_C; Phosphoribosylaminoimidazole carboxylase C-terminal domain (PF17769; HMM-score: 59.3)
  ATP-grasp (CL0179) ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 26.9)
  Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 24.6)
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 21.6)
  3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 15.8)
  NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 14.7)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 12.7)
  Rossmann-like; Rossmann-like domain (PF10727; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.304946
  • TAT(Tat/SPI): 0.024691
  • LIPO(Sec/SPII): 0.009547
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: AVN85233 NCBI
• UniProt:

⊟Protein sequence

• MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRVAEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNRIFEKDFLSNKSQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEADLEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPARISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIEAKHNRKMGHVTLFSDVPDSVDELGEGIDF

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Expression data

• PneumoExpress: SPV_0060 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications