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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 26-AUG-2017

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae OXC141
  • locus tag: SPNOXC01130 [new locus tag: SPNOXC_RS00655 ]
  • pan locus tag?: PNEUPAN000739000
  • symbol: SPNOXC01130
  • pan gene symbol?: pavB
  • synonym:
  • product: cell wall surface anchor family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPNOXC01130 [new locus tag: SPNOXC_RS00655 ]
  • symbol: SPNOXC01130
  • product: cell wall surface anchor family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 112642..115215
  • length: 2574
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: FQ312027 (112642..115215) NCBI
  • BioCyc: see SPNOXC_RS00655
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAATTCAATCCAAATCAAAGATATACTCGTTGGTCTATTCGCCGTCTCAGTGTCGGT
    GTTGCCTCAGTTGTTGTGGCTAGTGGCTTCTTTGTCCTAGTTGGTCAGCCAAGTTCTGTA
    CGTGCCGATGTGGTCAATCCGACCCCAGGTCAAGTCTTACCTGAAGAGACATCGGGAACG
    AAAGAGGGTGACTTATCAGAAAAACCAGGAGACACCGTTCTCACTCAAGCGAAACCTGAG
    GGCGTTACTGGAAATACGAATTCACTTCCGACACCTACAGAAAGAACTGAAGTGAGCGAG
    GAAACAAACTCTTCTAGTCTGGATACACTTTTTGAAAAAGATGAAGAAGCTCAAAAAAAT
    CCAGAGCTAACAGATGTCTTAAAAGAAACTGTAGATACAGCTGATGTGGATGGGACACAA
    GCAAGTCCAGCAGAAACTACTCCTGAACAAGTAAAAGGTGGAGTGAAAGAAAATACAAAA
    GACAGCATCGATGTTCCTGCTGCTTATCTTGAAAAAGCTGAAGGGAAAGGTCCTTTCACT
    GCCGGTGTAAACCAAGTAATTCCTTATGAACTATTCGCTGGTGATGGTATGTTAACTCGT
    CTATTACTAAAAGCTTCGGATAATGCTCCTTGGTCTGACAATGGTACTGCTAAAAATCCT
    GCTTTACCTCCTCTTGAAGGATTAACAAAAGGGAAATACTTCTATGAAGTAGACTTAAAT
    GGCAATACTGTTGGTAAACAAGGTCAAGCTTTAATTGATCAACTTCGAGCTAATGGTACT
    CAAACTTATAAAGCTACTGTTAAAGTTTACGGAAATAAAGACGGTAAAGCTGACTTGACT
    AATCTAGTTGCTACTAAAAATGTAGACATCAACATCAATGGATTAGTTGCTAAAGAAACT
    GTCGAAAAAGCCGTTGCAGACAACGTTAAAGACAGTATCGATGTTCCAGCAGCCTACCTA
    GAAAAAGCCAAGGGTGAAGGTCCATTCACAGCAGGTGTCAACCATGTGATTCCATACGAA
    CTCTTCGCAGGTGATGGCATGTTGACTCGTCTCTTGCTCAAGGCATCTGACAAGGCACCA
    TGGTCAGATAACGGCGACGCTAAAAACCCAGCTCTATCTCCACTAGGTGAAAACGTGAAG
    ACCAAAGGTCAATACTTCTATCAAGTAGCCTTGGACGGAAATGTAGCTGGCAAAGAAAAA
    CAAGCGCTCATTGACCAGTTCCGAGCAAATGGTACTCAAACTTACAGCGCTACAGTCAAT
    GTCTATGGTAACAAAGACGGTAAACCAGACTTGGACAACATCGTAGCAACTAAAAAAGTC
    ACTATTAACATAAACGGTTTAATTTCTAAAGAAACAGTTCAAAAAGCCGTTGCAGACAAC
    GTTAAAGACAGTATCGATGTTCCAGCAGCCTACCTAGAAAAAGCCAAGGGTGAAGGTCCA
    TTCACAGCAGGTGTCAACCATGTGATTCCATACGAACTCTTCGCAGGTGATGGCATGTTG
    ACTCGTCTCTTGCTCAAGGCATCTGACAAGGCACCATGGTCAGATAACGGCGACGCTAAA
    AACCCAGCTCTATCTCCACTAGGTGAAAACGTGAAGACCAAAGGTCAATACTTCTATCAA
    GTAGCCTTGGACGGAAATGTAGCTGGCAAAGAAAAACAAGCGCTCATTGACCAGTTCCGA
    GCAAATGGTACTCAAACTTACAGCGCTACAGTCAATGTCTATGGTAACAAAGACGGTAAA
    CCAGACTTGGACAACATCGTAGCAACTAAAAAAGTCACTATTAACATAAACGGTTTAATT
    TCTAAAGAAACAGTTCAAAAAGCCGTTGCAGACAACGTTAAAGACAGTATCGATGTTCCA
    GCAGCCTACCTAGAAAAAGCCAAGGGTGAAGGTCCATTCACAGCAGGTGTCAACCATGTG
    ATTCCATACGAACTCTTCGCAGGTGATGGCATGTTGACTCGTCTCTTGCTCAAGGCATCT
    GACAAGGCACCATGGTCAGATAACGGTGACGCTAAAAACCCAGCCCTATCTCCACTAGGT
    GAAAACGTGAAGACCAAAGGTCAATACTTCTATCAAGTAGCCTTGGACGGAAATGTAGCT
    GGCAAAGAAAAACAAGCGCTCATTGACCAGTTCCGAGCAAACGGTACTCAAACTTACAGC
    GCTACAGTCAATGTCTATGGTAACAAAGATGGTAAACCAGACTTGGACAACATCGTAGCA
    ACTAAAAAAGTCACTATTAAGATAAATGTTAAAGAAACATCAGACACAGCAAATGGTTCA
    TTATCACCTTCTAACTCTGGTTCTGGCGTGACTCCGATGAATCACAATCATGCTACAGGT
    ACTACAGATAGCATGCCTGCTGACACCATGACAAGTTCTACCAACACGATGGCAGGTGAA
    AACATGGCTGCTTCTGCTAACAAGATGTCTGATACGATGATATCAGAGGATAAAGCTATG
    CTACCAAATACTGGTGAGACTCAAACATCAATGGCAAGTATTGGTTTCCTTGGGCTTGCG
    CTTGCAGGTTTACTCGGTGGTCTAGGTTTGAAAAACAAAAAAGAAGAAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2574

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPNOXC01130 [new locus tag: SPNOXC_RS00655 ]
  • symbol: SPNOXC01130
  • description: cell wall surface anchor family protein
  • length: 857
  • theoretical pI: 4.93386
  • theoretical MW: 90937.1
  • GRAVY: -0.462077

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 45.8)
    and 7 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 22.5)
    Unknown function Enzymes of unknown specificity AIR synthase-related protein, sll0787 family (TIGR04049; HMM-score: 21.2)
    Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines ADP/ATP carrier protein family (TIGR00769; HMM-score: 14.5)
    isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 10.9)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein T (TIGR02851; HMM-score: 10.5)
    Signal transduction Regulatory functions DNA interactions stage V sporulation protein T (TIGR02851; HMM-score: 10.5)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS3 (TIGR01009; HMM-score: 6.8)
  • TheSEED  :
    • Cell wall surface anchor family protein
    Cell Wall and Capsule Gram-Positive cell wall components Sortase  Cell wall surface anchor family protein
  • ⊞PFAM:
    no clan defined SSURE; SSURE domain (PF11966; HMM-score: 1062)
    and 13 more
    YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 29.2)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 22)
    DUF6527; Family of unknown function (DUF6527) (PF20137; HMM-score: 20.1)
    Peptidase_CA (CL0125) Gln_amidase; Papain fold toxin 1, glutamine deamidase (PF15644; HMM-score: 19.9)
    no clan defined USP47_C; Ubiquitin carboxyl-terminal hydrolase 47 C-terminal (PF19718; HMM-score: 19.3)
    DUF2812; Protein of unknown function (DUF2812) (PF11193; HMM-score: 15.3)
    Flocculin; Flocculin repeat (PF00624; HMM-score: 14.9)
    NTF2 (CL0051) MlaC; MlaC protein (PF05494; HMM-score: 14.7)
    S11_L18p (CL0267) baeRF_family3; Bacterial archaeo-eukaryotic release factor family 3 (PF18845; HMM-score: 13.2)
    E-set (CL0159) LEA_2; Late embryogenesis abundant protein (PF03168; HMM-score: 12.5)
    DUF1735 (CL0594) DUF4973; Domain of unknown function (DUF4973) (PF16343; HMM-score: 12.1)
    ACT (CL0070) NIL; NIL domain (PF09383; HMM-score: 11.9)
    no clan defined Pro_CA; Carbonic anhydrase (PF00484; HMM-score: 10.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 2
  • ⊞SignalP: Signal peptide SP(Sec/SPI) length 42 aa
    • SP(Sec/SPI): 0.930917
    • TAT(Tat/SPI): 0.064848
    • LIPO(Sec/SPII): 0.001806
    • Cleavage Site: CS pos: 42-43. VRA-DV. Pr: 0.9799
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CBW31774 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADVVNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEETNSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTKDSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAKNPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGKADLTNLVATKNVDININGLVAKETVEKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTININGLISKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTININGLISKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMISEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPNOXC01130&oldid=120266"
  • This page was last edited on 20 January 2022, at 17:41.
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