⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 22-FEB-2023

⊟Summary

• organism: Streptococcus pneumoniae AP200
locus tag: SPAP_RS04780 [old locus tag: SPAP_0963 ]
pan locus tag^?: PNEUPAN001912000
symbol: proB
pan gene symbol^?: proB
• synonym:
• product: glutamate 5-kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPAP_RS04780 [old locus tag: SPAP_0963 ]
• symbol: proB
• product: glutamate 5-kinase
• replicon: chromosome
• strand: +
• coordinates: 918923..920032
• length: 1110
• essential: unknown

⊟Accession numbers

• Location: NC_014494 (918923..920032) NCBI
• BioCyc: SPAP_RS04780 BioCyc
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0822 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  
  ATGAAATACAAACGGATTGTCTTTAAGGTGGGTACTTCTTCTCTGACAAATGAGGATGGA
  AGTTTATCACGTAGTAAGGTAAAGGATATTACCCAGCAGTTGGCTATGCTGCACGAGGCT
  GGTCATGAGTTGATTTTGGTGTCTTCAGGTGCCATTGCGGCTGGTTTTGGAGCCTTAGGA
  TTTAAAAAGCGTCCGACTAAAATTGCTGATAAACAGGCTTCAGCAGCGGTAGGGCAGGGA
  CTTTTGTTGGAAGAATATACGACCAATCTTCTCTTGCGTCAAATCGTTTCTGCACAAATC
  TTGCTGACCCAAGATGACTTTGTGGATAAGCGTCGTTATAAAAATGCCCATCAGGCTTTG
  TCGGTTTTGCTCAACCGTGGGGCAATTCCTATCATCAATGAGAATGATAGTGTCGTTATT
  GATGAGCTCAAGGTTGGGGACAATGACACTCTAAGTGCTCAAGTAGCGGCGATGGTCCAA
  GCAGACCTTTTAGTTTTCTTGACAGATGTGGACGGTCTCTATACTGGAAATCCTAATTCA
  GATCCAAGAGCCAAACGCTTGGAGAGAATCGAGACCATCAATCGTGAGATTATTGATATG
  GCTGGTGGAGCTGGTTCGTCAAACGGAACTGGGGGTATGTTAACCAAAATCAAGGCTGCA
  ACTATCGCGACGGAATCAGGAGTTCCTGTTTATATCTGCTCATCCTTGAAATCAGATTCC
  ATGATTGAGGCGGCAGAGGAGACCGAGGATGGTTCTTACTTTGTTGCTCAAGAGAAGGGG
  CTTCGTACCCAGAAACAATGGCTTGCCTTCTATGCTCAGAGTCAAGGTTCTATTTGGGTT
  GATAAAGGGGCTGCGGAAGCTCTCTCTCAACATGGAAAGAGTCTTCTCTTATCTGGTATC
  GTTGAAGCAGAAGGAGTCTTTTCTTACGGTGATATCGTGACAGTATTTGATAAGGAAAGT
  GGAAAATCACTTGGAAAAGGACGCGTGCAATTTGGAGCATCTGCTTTGGAGGATATGTTG
  CGTTCTCAAAAAGCCAAGGGTGTCTTGATTTACCGTGACGACTGGATTTCCATTACTCCT
  GAAATCCAACTACTTTTTACAGAATTTTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1110
  

⊟Protein

⊟General

• locus tag: SPAP_RS04780 [old locus tag: SPAP_0963 ]
• symbol: ProB
• description: glutamate 5-kinase
• length: 369
• theoretical pI: 5.30485
• theoretical MW: 39901.1
• GRAVY: -0.098103

⊟Function

• ⊞⊞reaction:
  EC 2.7.2.11?  ExPASy
  Glutamate 5-kinase ATP + L-glutamate = ADP + L-glutamate 5-phosphate
• ⊞⊞TIGRFAM:
  Metabolism Amino acid biosynthesis Glutamate family glutamate 5-kinase (TIGR01027; EC 2.7.2.11; HMM-score: 447.1)
  and 10 more
  Metabolism Amino acid biosynthesis Glutamate family delta l-pyrroline-5-carboxylate synthetase (TIGR01092; HMM-score: 153.9)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 45.5)
  Metabolism Energy metabolism Amino acids and amines carbamate kinase (TIGR00746; EC 2.7.2.2; HMM-score: 36.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions UMP kinase (TIGR02075; EC 2.7.4.22; HMM-score: 36.4)
  Metabolism Amino acid biosynthesis Glutamate family acetylglutamate kinase (TIGR00761; EC 2.7.2.8; HMM-score: 35.8)
  Metabolism Amino acid biosynthesis Aspartate family aspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 34.5)
  arCOG04150 universal archaeal PUA-domain protein (TIGR03684; HMM-score: 32.4)
  Metabolism Amino acid biosynthesis Aspartate family aspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 29.8)
  Metabolism Amino acid biosynthesis Aspartate family putative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 22.2)
  uncharacterized domain 2 (TIGR00451; HMM-score: 17.6)
• TheSEED: see SPAP_0963
• ⊞⊞PFAM:
  no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 143.9)
  and 1 more
  PUA (CL0178) PUA; PUA domain (PF01472; HMM-score: 58.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• ⊞⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9655
  • Cytoplasmic Membrane Score: 0.0117
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.0223
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.028987
  • TAT(Tat/SPI): 0.005043
  • LIPO(Sec/SPII): 0.002062
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000875744 NCBI
• UniProt:

⊟Protein sequence

• MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKGRVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0822 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications