From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 17-JAN-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_1609 [new locus tag: SPAP_RS07940 ]
  • pan locus tag?: PNEUPAN002971000
  • symbol: SPAP_1609
  • pan gene symbol?: merA
  • synonym:
  • product: Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component,-like enzyme

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_1609 [new locus tag: SPAP_RS07940 ]
  • symbol: SPAP_1609
  • product: Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component,-like enzyme
  • replicon: chromosome
  • strand: +
  • coordinates: 1515333..1516649
  • length: 1317
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTTAACTTATGATTTAATCGTTATCGGATTTGGTAAAGCTGGTAAAACACTAGCAGGT
    AAATTGGCTTCAGCTGGCAAAAAAGTTGCCCTCGTTGAACGTAGCAAAGCTATGTACGGT
    GGAACTTGTATCAACATCGGTTGTATCCCAACTAAAACTTTGCTGGTTGCTGCTGAGAAA
    GACTTGTCTTTTGAAGAAGTCATTGCTACCAAAAACACGATCACTGGTCGCCTCAACGGT
    AAAAACTATACGACTGTTGCTGGTACTGGCGTCAATATCTTTGATGCTGAAGCTCACTTC
    CTTTCAAATAAAGTCATCGAAATCCAAGCTGGTGATGAAAAACAAGAATTGACTGCTGAA
    ACTATCGTCATCAACACTGGTGCTGTTTCAAACGTCTTGCCAATCCCTGGACTTGCTACA
    AGCAAAAACGTCTTTGACTCAACAGGTATCCAAAGCTTGGATAAATTGCCTGAAAAACTT
    GGAGTCCTTGGTGGCGGAAATATCGGTCTTGAATTTGCTGGCCTTTACAATAAACTAGGA
    AGCAAGGTTACAGTCCTAGATGCCTTGGATACATTCCTACCTCGTGCAGAACCTTCCATC
    GCAGCTCTTGCTAAACAATACATGGAAGAAGACGGTATTGAATTGCTTCAAAATATCCAT
    ACTACTGAAATTAAAAACGACGGTGACCAAGTGCTTGTCGTAACTGAAGACGAAACTTAC
    CGTTTCGACGCCCTTCTCTACGCAACTGGACGCAAACCAAATGTAGAACCACTTCAACTT
    GAAAATACAGATATTGAACTAACTGAACGTGGCGCTATTAAAGTAGATAAACACTGTCAA
    ACAAACGTTCCTGGTGTCTTTGCAGTTGGAGATGTCAACGGTGGTCTTCAATTTACTTAC
    ATTTCACTTGATGACTTCCGTGTTGTTTACAGCTACCTTGCTGGAGATGGCAGCTACACA
    CTTGAGGACCGTCTCAATGTGCCAAATACTATGTTCATCACACCTGCACTTTCACAAGTT
    GGTTTGACTGAAAGCCAAGCAGCTGATTTGAAACTTCCATACGCAGTGAAAGAAATCCCT
    GTTGCAGCCATGCCTCGTGGTCACGTAAATGGAGACCTTCGCGGAGCTTTCAAAGCTGTT
    GTTAATACTGAAACAAAAGAAATTCTTGGTGCAAGCATCTTCTCAGAAGGTTCTCAAGAA
    ATCATCAACATCATTACTGTTGCTATGGACAACAAGATTCCTTACACTTACTTCACAAAA
    CAAATCTTCACTCACCCAACCTTGGCTGAGAACTTGAATGACTTGTTTGCGATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1317

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_1609 [new locus tag: SPAP_RS07940 ]
  • symbol: SPAP_1609
  • description: Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component,-like enzyme
  • length: 438
  • theoretical pI: 4.61112
  • theoretical MW: 47161.5
  • GRAVY: 0.0232877

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 319.8)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 291.9)
    and 42 more
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 225.6)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 211.3)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 191.9)
    thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 141.9)
    trypanothione-disulfide reductase (TIGR01423; EC 1.8.1.12; HMM-score: 134)
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 97.3)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 70.6)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 62.1)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 54.5)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 54.5)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 48.4)
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 47.5)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 42)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 36.6)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 27)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 26.9)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 25.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 25)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 23)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 21.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 19.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 19)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides L-aspartate oxidase (TIGR00551; EC 1.4.3.16; HMM-score: 18.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 18.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other phytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 16.8)
    mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 16.6)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-galactopyranose mutase (TIGR00031; EC 5.4.99.9; HMM-score: 15.9)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 15.8)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 15.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 15.1)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 15)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, monomeric form (TIGR01377; HMM-score: 14.1)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 14.1)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 12.9)
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 11.5)
    flavin-dependent oxidoreductase, MSMEG_0569 family (TIGR04046; HMM-score: 11.3)
    lycopene cyclase family protein (TIGR01790; HMM-score: 11.1)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 10.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 10.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other C-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 10.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 8.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 7.6)
  • ⊞TheSEED  :
    • Mercuric ion reductase (EC 1.16.1.1)
    • PF00070 family, FAD-dependent NAD(P)-disulphide oxidoreductase
    • Putative Dihydrolipoamide dehydrogenase (EC 1.8.1.4)
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercuric reductase  Mercuric ion reductase (EC 1.16.1.1)
    and 2 more
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercuric reductase  PF00070 family, FAD-dependent NAD(P)-disulphide oxidoreductase
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercury resistance operon  Mercuric ion reductase (EC 1.16.1.1)
  • ⊞PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 200.8)
    and 24 more
    Reductase_C (CL0608) Pyr_redox_dim; Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain (PF02852; HMM-score: 86.9)
    NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 77.3)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 56.8)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 24.7)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 24.6)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 23.6)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 22)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 21.1)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 20.7)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 18.7)
    XdhC_C; XdhC Rossmann domain (PF13478; HMM-score: 17.8)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 17.1)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 16.9)
    Thi4; Thi4 family (PF01946; HMM-score: 16.5)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 16.2)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 15.8)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 15.5)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 14.9)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 14.2)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 13.7)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 13.2)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 13)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 12.6)
    GDI; GDP dissociation inhibitor (PF00996; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.049236
    • TAT(Tat/SPI): 0.013996
    • LIPO(Sec/SPII): 0.020792
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ADM85193 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCINIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVNIFDAEAHFLSNKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKLGVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQNIHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVDKHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFITPALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGASIFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPAP_1609&oldid=43800"