⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 17-JAN-2018

⊟Summary

• organism: Streptococcus pneumoniae AP200
locus tag: SPAP_1609 [new locus tag: SPAP_RS07940 ]
pan locus tag^?: PNEUPAN002971000
symbol: SPAP_1609
pan gene symbol^?: merA
• synonym:
• product: Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component,-like enzyme

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPAP_1609 [new locus tag: SPAP_RS07940 ]
• symbol: SPAP_1609
• product: Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component,-like enzyme
• replicon: chromosome
• strand: +
• coordinates: 1515333..1516649
• length: 1317
• essential: unknown

⊟Accession numbers

• Location: CP002121 (1515333..1516649) NCBI
• BioCyc: see SPAP_RS07940
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1415 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGTTAACTTATGATTTAATCGTTATCGGATTTGGTAAAGCTGGTAAAACACTAGCAGGT
  AAATTGGCTTCAGCTGGCAAAAAAGTTGCCCTCGTTGAACGTAGCAAAGCTATGTACGGT
  GGAACTTGTATCAACATCGGTTGTATCCCAACTAAAACTTTGCTGGTTGCTGCTGAGAAA
  GACTTGTCTTTTGAAGAAGTCATTGCTACCAAAAACACGATCACTGGTCGCCTCAACGGT
  AAAAACTATACGACTGTTGCTGGTACTGGCGTCAATATCTTTGATGCTGAAGCTCACTTC
  CTTTCAAATAAAGTCATCGAAATCCAAGCTGGTGATGAAAAACAAGAATTGACTGCTGAA
  ACTATCGTCATCAACACTGGTGCTGTTTCAAACGTCTTGCCAATCCCTGGACTTGCTACA
  AGCAAAAACGTCTTTGACTCAACAGGTATCCAAAGCTTGGATAAATTGCCTGAAAAACTT
  GGAGTCCTTGGTGGCGGAAATATCGGTCTTGAATTTGCTGGCCTTTACAATAAACTAGGA
  AGCAAGGTTACAGTCCTAGATGCCTTGGATACATTCCTACCTCGTGCAGAACCTTCCATC
  GCAGCTCTTGCTAAACAATACATGGAAGAAGACGGTATTGAATTGCTTCAAAATATCCAT
  ACTACTGAAATTAAAAACGACGGTGACCAAGTGCTTGTCGTAACTGAAGACGAAACTTAC
  CGTTTCGACGCCCTTCTCTACGCAACTGGACGCAAACCAAATGTAGAACCACTTCAACTT
  GAAAATACAGATATTGAACTAACTGAACGTGGCGCTATTAAAGTAGATAAACACTGTCAA
  ACAAACGTTCCTGGTGTCTTTGCAGTTGGAGATGTCAACGGTGGTCTTCAATTTACTTAC
  ATTTCACTTGATGACTTCCGTGTTGTTTACAGCTACCTTGCTGGAGATGGCAGCTACACA
  CTTGAGGACCGTCTCAATGTGCCAAATACTATGTTCATCACACCTGCACTTTCACAAGTT
  GGTTTGACTGAAAGCCAAGCAGCTGATTTGAAACTTCCATACGCAGTGAAAGAAATCCCT
  GTTGCAGCCATGCCTCGTGGTCACGTAAATGGAGACCTTCGCGGAGCTTTCAAAGCTGTT
  GTTAATACTGAAACAAAAGAAATTCTTGGTGCAAGCATCTTCTCAGAAGGTTCTCAAGAA
  ATCATCAACATCATTACTGTTGCTATGGACAACAAGATTCCTTACACTTACTTCACAAAA
  CAAATCTTCACTCACCCAACCTTGGCTGAGAACTTGAATGACTTGTTTGCGATTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1317
  

⊟Protein

⊟General

• locus tag: SPAP_1609 [new locus tag: SPAP_RS07940 ]
• symbol: SPAP_1609
• description: Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component,-like enzyme
• length: 438
• theoretical pI: 4.61112
• theoretical MW: 47161.5
• GRAVY: 0.0232877

⊟Function

• ⊞⊞TIGRFAM:
  dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 319.8)
  Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 291.9)
  and 42 more
  Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 225.6)
  mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 211.3)
  Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 191.9)
  thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 141.9)
  trypanothione-disulfide reductase (TIGR01423; EC 1.8.1.12; HMM-score: 134)
  Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 97.3)
  Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 70.6)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 62.1)
  Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 54.5)
  Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 54.5)
  Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 48.4)
  pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 47.5)
  Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 42)
  glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 36.6)
  putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 27)
  putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 26.9)
  Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 25.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 25)
  glutamate synthase, small subunit (TIGR01318; HMM-score: 23)
  FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 21.7)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 19.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 19)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides L-aspartate oxidase (TIGR00551; EC 1.4.3.16; HMM-score: 18.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 18.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other phytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 16.8)
  mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 16.6)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-galactopyranose mutase (TIGR00031; EC 5.4.99.9; HMM-score: 15.9)
  mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 15.8)
  Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 15.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 15.1)
  Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 15)
  Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, monomeric form (TIGR01377; HMM-score: 14.1)
  nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 14.1)
  squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 12.9)
  Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 11.5)
  flavin-dependent oxidoreductase, MSMEG_0569 family (TIGR04046; HMM-score: 11.3)
  lycopene cyclase family protein (TIGR01790; HMM-score: 11.1)
  Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 10.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 10.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other C-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 10.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 8.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 7.6)
• ⊞⊞TheSEED  :
  • Mercuric ion reductase (EC 1.16.1.1)
  • PF00070 family, FAD-dependent NAD(P)-disulphide oxidoreductase
  • Putative Dihydrolipoamide dehydrogenase (EC 1.8.1.4)
  Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercuric reductase  Mercuric ion reductase (EC 1.16.1.1)
  and 2 more
  Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercuric reductase  PF00070 family, FAD-dependent NAD(P)-disulphide oxidoreductase
  Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercury resistance operon  Mercuric ion reductase (EC 1.16.1.1)
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 200.8)
  and 24 more
  Reductase_C (CL0608) Pyr_redox_dim; Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain (PF02852; HMM-score: 86.9)
  NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 77.3)
  Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 56.8)
  FAD_binding_2; FAD binding domain (PF00890; HMM-score: 24.7)
  3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 24.6)
  2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 23.6)
  TrkA_N; TrkA-N domain (PF02254; HMM-score: 22)
  GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 21.1)
  HI0933_like; HI0933-like protein (PF03486; HMM-score: 20.7)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 18.7)
  XdhC_C; XdhC Rossmann domain (PF13478; HMM-score: 17.8)
  FAD_binding_3; FAD binding domain (PF01494; HMM-score: 17.1)
  NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 16.9)
  Thi4; Thi4 family (PF01946; HMM-score: 16.5)
  DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 16.2)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 15.8)
  ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 15.5)
  NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 14.9)
  Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 14.2)
  UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 13.7)
  AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 13.2)
  Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 13)
  FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 12.6)
  GDI; GDP dissociation inhibitor (PF00996; HMM-score: 11.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.049236
  • TAT(Tat/SPI): 0.013996
  • LIPO(Sec/SPII): 0.020792
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: ADM85193 NCBI
• UniProt:

⊟Protein sequence

• MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCINIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVNIFDAEAHFLSNKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKLGVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQNIHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVDKHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFITPALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGASIFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_1415 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications