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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 07-FEB-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_RS02025 [old locus tag: SPAP_0412 ]
  • pan locus tag?: PNEUPAN001250000
  • symbol: SPAP_RS02025
  • pan gene symbol?: vraT
  • synonym: liaF
  • product: cell wall-active antibiotics response protein LiaF

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_RS02025 [old locus tag: SPAP_0412 ]
  • symbol: SPAP_RS02025
  • product: cell wall-active antibiotics response protein LiaF
  • replicon: chromosome
  • strand: +
  • coordinates: 383417..384115
  • length: 699
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_014494 (383417..384115) NCBI
  • BioCyc: SPAP_RS02025 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0350 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGAAAATTTAAAATCTTTTTATTTATCGAAGCCTGTCTTCTGACAGGAGCTCTGATT
    TTGATGGTATCAGAGCATTTTTCGCGTTTTCTGCTGATACTATTCCTCTTTTTGCTTTTG
    ATTCGCTACTACACTGGTAAAGAGGGAAATAATCTTCTTTTAGTAGCGGCAACCATTCTC
    TTCTTTTTCATCGTTATGCTCAATCCTTTTGTGATTCTAGCTATTTTTGTTGCGGTTATC
    TATAGCCTCTTTCTTCTTTACCCGATGATGAACCAGGAAAAAGAGCAGACCAATTTGGTT
    TTTGAAGAGGTCGTGACGGTTAAGAAGGAGAAAAATCGTTGGTTTGGAAATCTTCATCAT
    TTTTCAAGCTACCAGACTTGCCAATTCGATGATATCAATCTCTTTCGCTTCATGGGCAAG
    GACACTATTCATCTGGAGAGGGTCATCTTAACCAATCATGACAATGTCATTATCCTCAGA
    AAGATGGTAGGAACGACCAAAATCATCGTACCTGTAGATGTGGAAGTCAGTCTCAGCGTT
    AACTGTCTCTATGGGGATTTGATTTTTTTCAACCAGCCCAAGCGAGCCCTCCGCAATGAA
    CACTATCATCAAGAAACAAAAGACTATCTCAAGAGTAACAAGAGTGTCAAGATTTTCTTG
    ACCACTATGATTGGTGATGTGGAGGTGGTTAGAGGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    699

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_RS02025 [old locus tag: SPAP_0412 ]
  • symbol: SPAP_RS02025
  • description: cell wall-active antibiotics response protein LiaF
  • length: 232
  • theoretical pI: 9.05572
  • theoretical MW: 27192.1
  • GRAVY: 0.421121

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 11.2)
    Metabolism Energy metabolism Electron transport cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 11.2)
  • TheSEED: see SPAP_0412
  • ⊞PFAM:
    Pec_lyase-like (CL0268) DUF2154; Cell wall-active antibiotics response 4TMS YvqF (PF09922; HMM-score: 115.1)
    and 2 more
    O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 14.8)
    Peptidase_MA (CL0126) Peptidase_M50B; Peptidase M50B-like (PF13398; HMM-score: 11.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 2
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005349
    • TAT(Tat/SPI): 0.000217
    • LIPO(Sec/SPII): 0.149883
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001224636 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYYTGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFEEVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILRKMVGTTKIIVPVDVEVSLSVNCLYGDLIFFNQPKRALRNEHYHQETKDYLKSNKSVKIFLTTMIGDVEVVRG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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