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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 07-FEB-2021

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_RS11080 [old locus tag: SPAP_2241 ]
  • pan locus tag?: PNEUPAN003920000
  • symbol: SPAP_RS11080
  • pan gene symbol?: thiY
  • synonym:
  • product: ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_RS11080 [old locus tag: SPAP_2241 ]
  • symbol: SPAP_RS11080
  • product: ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2087584..2088591
  • length: 1008
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_014494 (2087584..2088591) NCBI
  • BioCyc: SPAP_RS11080 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_2025 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAGAAAACATGGAAAGTGTTTTTAACGCTTGTAACAGCTCTTGTAGCTGTTGTGCTT
    GTGGCCTGTGGTCAAGGAACTGCTTCTAAAGACAACAAAGAGGCAGAACTTAAGAAGGTT
    GACTTTATCCTAGACTGGACACCAAATACCAACCACACAGGGCTTTATGTTGCCAAGGAA
    AAAGGTTATTTCAAAGAAGCTGGAGTGGATGTTGATTTGAAATTGCCACCAGAAGAAAGT
    TCTTCTGACTTGGTTATCAACGGAAAGGCACCATTTGCAGTGTATTTCCAAGACTACATG
    GCTAAGAAATTGGAAAAAGGAGCAGGAATCACTGCCGTTGCAGCTATTGTTGAACACAAT
    ACATCAGGAATCATCTCTCGTAAATCTGATAATGTAAGCAGTCCAAAAGACTTGGTTGGT
    AAGAAATATGGGACATGGAATGACCCAACTGAACTTGCTATGTTGAAAACCTTGGTAGAA
    TCTCAAGGTGGAGACTTTGAGAAGGTTGAAAAAGTACCAAATAACGACTCAAACTCAATC
    ACACCGATTGCCAATGGGGTCTTTGATACTGCTTGGATTTACTACGGTTGGGATGGTATC
    CTTGCTAAATCTCAAGGTGTAGATGCTAACTTCATGTACTTGAAAGACTATGTCAAGGAG
    TTTGACTACTATTCACCAGTTATCATCGCAAACAACGACTATCTGAAAGATAACAAAGAA
    GAAGCTCGCAAAGTCATCCAAGCCATCAAAAAAGGCTACCAATATGCCATGGAACATCCA
    GAAGAAGCTGCAGATATTCTCATCAAGAATGCACCTGAACTCAAGGAAAAACGTGACTTT
    GTCATCGAATCTCAAAAATACTTGTCAAAAGAATACGCAAGCGACAAGGAAAAATGGGGT
    CAATTTGACGCAGCTCGCTGGAATGCTTTCTACAAATGGGATAAAGAAAATGGTATCCTT
    AAAGAAGACTTGACAGACAAAGGCTTCACCAACGAATTTGTGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1008

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_RS11080 [old locus tag: SPAP_2241 ]
  • symbol: SPAP_RS11080
  • description: ABC transporter substrate-binding protein
  • length: 335
  • theoretical pI: 5.20478
  • theoretical MW: 37776.5
  • GRAVY: -0.493134

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Other ABC transporter, substrate-binding protein, aliphatic sulfonates family (TIGR01728; HMM-score: 47.6)
  • TheSEED: see SPAP_2241
  • ⊞PFAM:
    PBP (CL0177) NMT1; NMT1/THI5 like (PF09084; HMM-score: 254.3)
    and 3 more
    NMT1_2; NMT1-like family (PF13379; HMM-score: 16.2)
    no clan defined DUF6511; Family of unknown function (DUF6511) (PF20121; HMM-score: 12.8)
    PBP (CL0177) VitK2_biosynth; Menaquinone biosynthesis (PF02621; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • ⊞SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
    • SP(Sec/SPI): 0.002789
    • TAT(Tat/SPI): 0.000081
    • LIPO(Sec/SPII): 0.996954
    • Cleavage Site: CS pos: 22-23. LVA-CG. Pr: 0.9972
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000754546 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFILDWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAKKLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVESQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYVKEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKEKRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFVK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for TIGR4

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_2025 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPAP_RS11080&oldid=42149"
  • This page was last edited on 20 January 2022, at 14:40.
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