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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 26-OCT-2021

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae R6
  • locus tag: SPR_RS08300 [old locus tag: spr1668 ]
  • pan locus tag?: PNEUPAN003347000
  • symbol: SPR_RS08300
  • pan gene symbol?: galK
  • synonym:
  • product: galactokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPR_RS08300 [old locus tag: spr1668 ]
  • symbol: SPR_RS08300
  • product: galactokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1640591..1641769
  • length: 1179
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_003098 (1640591..1641769) NCBI
  • BioCyc: see spr1668
  • MicrobesOnline: see spr1668
  • PneumoBrowse for strain D39V: SPV_1634 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGACACAACATCTTACTGCTGAAACTCTTCGCAAAGACTTTCTTGCTGTTTTTGGTCAA
    GAAGTAGACCAAACATTCTTTTCACCAGGTCGTATCAATTTGATTGGTGAACACACGGAC
    TATAATGGTGGACATGTATTTCCAGTTGCTATTTCCTTGGGAACATACGGCGCAGCTCGC
    AAGCGTGACGACCAAGTCTTGCGTTTCTACTCAGCCAACTTTGAGGACAAGGGCATTATC
    GAAGTGCCTCTTGCTGACCTCAAGTTTGAAAAAGAGCACAACTGGACCAACTATCCAAAA
    GGGGTTCTTCATTTCTTGCAAGAAGCTGGGCACGTGATTGACAAAGGTTTTGATTTTTAT
    GTTTATGGGAATATCCCAAATGGTTCTGGCTTGTCTTCTTCAGCATCCTTGGAGATCTTG
    ACAGGAGTCGTGGCAGAGCATCTCTTTGATTTAAAACTAGAGCGTCTCGATTTGGTTAAA
    ATCGGAAAACAAACAGAAAATAACTTTATCGGAGTCAACTCTGGTATCATGGACCAGTTT
    GCTATTGGTATGGGGGCTGATCAACGTGCTATTTACCTTGATACCAACACCTTAGAGTAT
    GATTTGGTGCCACTTGATTTGAAGGACAATGTCGTTGTTATCATGAACACCAACAAACGC
    CGTGAATTGGCGGACTCTAAATACAATGAACGTCGTGCTGAGTGTGAAAAAGCAGTGGAA
    GAATTGCAAGTTGCCTTGGATATTCAGACCCTGGGTGAATTGGACGAGTGGGCCGTTGAC
    CAATATAGCTATCTGATTAAAGATGAAAATCGTTTGAAACGTGCTCGCCATGCTGTGCTT
    GAAAACCAACGTACCCTTAAAGCTCAAGCAGCCCTTCAAGCAGGTGATTTGGAAACATTT
    GGTCGTTTGATGAATGCGTCACACGTTTCTCTGGAGCATGATTATGAAGTAACTGGTTTG
    GAATTAGATACCCTTGTTCACACAGCTTGGGCACAAGAAGGAGTTCTCGGTGCTCGTATG
    ACAGGGGCTGGTTTTAGTGGCTGTGCCATTGCCTTGGTTCAAAAAGATACTGTTGAGGCC
    TTTAAGGAAGCTGTAGGCAAACACTACGAGGAAGTAGTTGGATACGCTCCAAGCTTCTAT
    ATCGCTGAAGTTGCAGGTGGCACTCGCGTCCTTGACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1179

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPR_RS08300 [old locus tag: spr1668 ]
  • symbol: SPR_RS08300
  • description: galactokinase
  • length: 392
  • theoretical pI: 4.71021
  • theoretical MW: 43671.8
  • GRAVY: -0.2375

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.7.1.6?  ExPASy
    Galactokinase ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate
  • ⊞TIGRFAM:
    Metabolism Energy metabolism Sugars galactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 393.1)
    and 3 more
    Metabolism Central intermediary metabolism Other mevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 84.5)
    Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 20.2)
    Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 14.2)
  • TheSEED: see spr1668
  • ⊞PFAM:
    S5 (CL0329) GalKase_gal_bdg; Galactokinase galactose-binding signature (PF10509; HMM-score: 74.1)
    and 2 more
    GHMP_C (CL0677) GHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 51)
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 47.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004784
    • TAT(Tat/SPI): 0.000599
    • LIPO(Sec/SPII): 0.000731
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000191983 NCBI
  • UniProt: see spr1668

⊟Protein sequence[edit | edit source]

  • MTQHLTAETLRKDFLAVFGQEVDQTFFSPGRINLIGEHTDYNGGHVFPVAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGVLHFLQEAGHVIDKGFDFYVYGNIPNGSGLSSSASLEILTGVVAEHLFDLKLERLDLVKIGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTNKRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRARHAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEGVLGARMTGAGFSGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SPR_RS08290 < SPR_RS08295 < SPR_RS08300

⊟Regulation[edit | edit source]

  • regulator: CcpA, GalR see spr1668

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_1634 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPR_RS08300&oldid=128216"
  • This page was last edited on 20 January 2022, at 18:00.
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