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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae G54
  • locus tag: SPG_0053 [new locus tag: SPG_RS00265 ]
  • pan locus tag?: PNEUPAN000672000
  • symbol: purM
  • pan gene symbol?: purM
  • synonym:
  • product: phosphoribosylformylglycinamidine cyclo-ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPG_0053 [new locus tag: SPG_RS00265 ]
  • symbol: purM
  • product: phosphoribosylformylglycinamidine cyclo-ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 49369..50391
  • length: 1023
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP001015 (49369..50391) NCBI
  • BioCyc: see SPG_RS00265
  • MicrobesOnline: 5755322 MicrobesOnline
  • PneumoBrowse for strain D39V: SPV_0054 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACAAATAAAAATGCATATGCCCAGTCGGGTGTGGATGTTGAAGCGGGTTATGAAGTT
    GTTGAACGGATTAAAAAGCACGTGGCCCGTACGGAGCGTGCAGGTGTCATGGGAGCTCTT
    GGTGGCTTTGGTGGTATGTTTGACCTTTCCAAGACTGGGGTTAAAGAACCCGTCTTGATT
    TCAGGGACTGACGGTGTCGGAACCAAGCTCATGTTGGCTATCAAGTACGACAAGCACGAT
    ACCATCGGGCAGGACTGTGTGGCCATGTGTGTCAACGACATCATTGCTGCAGGTGCGGAA
    CCCCTCTATTTTCTCGACTACGTAGCGACAGNGAAGAATGAACCAGCTAAGCTAGAACAA
    GTGGTTGCTGGTGTGGCAGAAGGTTGTGTGCAGGCTGGTGCTGCCCTCATCGGTGGGGAA
    ACGGCTGAAATGCCGGGCATGTACGGCGAAGACGACTATGACTTGGCTGGTTTTGCGGTC
    GGTGTGGCTGAAAAATCTCAAATCATTGACGGTTCAAAGGTGGTAGAGGGAGATGTTCTT
    CTCGGACTTGTTTCAAGTGGGATTCACTCAAATGGTTACTCTTTGGTTCGTCGTGTCTTT
    GCGGATTACACAGGTGAGGAAGTCCTACCAGAATTGGAAGGCAAGAAACTTAAGGAAGTT
    CTACTTGAGCCGACTCGTATCTATGTCAAGGCTGTCTTGCCGCTCATCAAAGAAGAGTTG
    GTCAACGGCATTGCCCACATCACAGGTGGTGGCTTTATCGAAAATGTCCCTCGTATGTTT
    GCAGATGACCTAGCTGCTGAAATTGATGAAAGCAAAGTTCCAGTGCTTCCAATTTTCAAA
    GCCCTTGAAAAATACGGTCAGATTAAACACGAAGAAATGTTTGAAATCTTCAATATGGGT
    GTGGGACTCATGTTGGCGGTCAGCCCTGAAAATGTAGAGCGTGTAAAAGAATTGTTGGAT
    GAAGCAGTCTATGAAATTGGTCGCATCGTCAAGAAAGAAAACGAAAGTGTCATTATCAAA
    TGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1023

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPG_0053 [new locus tag: SPG_RS00265 ]
  • symbol: PurM
  • description: phosphoribosylformylglycinamidine cyclo-ligase
  • length: 340
  • theoretical pI: 4.45605
  • theoretical MW: 36472.8
  • GRAVY: 0.0737463

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 6.3.3.1?  ExPASy
    Phosphoribosylformylglycinamidine cyclo-ligase ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole
  • ⊞TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine cyclo-ligase (TIGR00878; EC 6.3.3.1; HMM-score: 449.8)
    and 9 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate kinase (TIGR01379; EC 2.7.4.16; HMM-score: 52)
    selenide, water dikinase (TIGR00476; EC 2.7.9.3; HMM-score: 37.8)
    hydrogenase expression/formation protein HypE (TIGR02124; HMM-score: 30.8)
    Metabolism Energy metabolism Methanogenesis putative methanogenesis marker protein 2 (TIGR03267; HMM-score: 29.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase II (TIGR01736; EC 6.3.5.3; HMM-score: 17.6)
    Unknown function Enzymes of unknown specificity AIR synthase-related protein, MSMEG_0567 C-terminal family (TIGR04050; HMM-score: 16.5)
    Unknown function Enzymes of unknown specificity AIR synthase-related protein, sll0787 family (TIGR04049; HMM-score: 15.4)
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 12.8)
    phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 12.3)
  • TheSEED  :
    • Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1)
    Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1)
  • ⊞PFAM:
    no clan defined AIRS_C; AIR synthase related protein, C-terminal domain (PF02769; HMM-score: 127.2)
    and 2 more
    AIRS; AIR synthase related protein, N-terminal domain (PF00586; HMM-score: 74.1)
    HTH (CL0123) DUF4423; Domain of unknown function (DUF4423) (PF14394; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9858
    • Cytoplasmic Membrane Score: 0.001
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0131
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.030712
    • TAT(Tat/SPI): 0.025388
    • LIPO(Sec/SPII): 0.001839
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACF56494 NCBI
  • UniProt: B5E5J6 UniProt

⊟Protein sequence[edit | edit source]

  • MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFGGMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPLYFLDYVATXKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAVGVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLKEVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVLPIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKENESVIIK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: purC > purL > purF > purM > purN

⊟Regulation[edit | edit source]

  • ⊞regulator: PurR (repression) regulon
    PurR(TF)important in Purine metabolism; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0054 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPG_0053&oldid=73040"
  • This page was last edited on 20 January 2022, at 14:51.
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