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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae Hungary19A-6
  • locus tag: SPH_2238 [new locus tag: SPH_RS10965 ]
  • pan locus tag?: PNEUPAN000088000
  • symbol: SPH_2238
  • pan gene symbol?: —
  • synonym:
  • product: transposase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPH_2238 [new locus tag: SPH_RS10965 ]
  • symbol: SPH_2238
  • product: transposase
  • replicon: chromosome
  • strand: -
  • coordinates: 2053542..2054798
  • length: 1257
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP000936 (2053542..2054798) NCBI
  • BioCyc: see SPH_RS10965
  • MicrobesOnline: 5697058 MicrobesOnline
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGAACAATTACATTTTATCACAAAACTACTCGATATCAAAGACACAAATATCCAAATT
    ATAGATGTCGTCAATAGGGATTCACACAAGGAAATCATCGCCAAACTGGACTACGACGCC
    CCATCTTGCCCTGAGTGCGGAAACCAATTGAAGAAATATGACTTTCAAAAACCTTCTAAA
    ATTCCTTATCTTGAAACGACTGGTATGCCTACTAGAATTCTCCTTAGAAAGCGTCGATTC
    AAGTGCTATCACTGTTCAAAAATGATGGTTGCTGAAACTTCTATCGTCAAGAAGAATCAC
    CAAATCCCTCGTATCATCAACCAAAAGATTGCTCAAAAGTTAATTGAAAAGATTTCTATG
    ACTGATATTGCCCATCAGCTTTCCATCTCAACTTCAACTGTTATTCGTAAGCTCAATGAT
    TTTCACTTTAAACATGATTTTTCTTGTCTTCCTGAGATTATGTCTTGGGATGAGTATGCT
    TTTACAAAGGGAAAGATGAGCTTCATTGCGCAAGATTTTAACAATCTCAATATCATCACT
    GTTCTTGAGGGAAGAACACAAGCTATCATTCGAAATCACTTTCTTAAATATGATAGAGCC
    GTCCGATGTCGCGTCAAAATTATTACTATGGATATGTTTAGTCCTTACTATGACTTAGCT
    AGACAACTTTTCCCATGTGCTAAAATCGTGTTGGATCGCTTTCACATTGTACAACATCTT
    AGCCGTGCTATGAGTCGTGTGCGTGTTCAAATCATGAATCAATTGGATCGAAAGTCTCAT
    GAATACAAGGCTATCAAGCGCTACTGGAAGCTCATACAACAGGATAGTCGTAAACTCAGC
    GATAAACATTTTTATCGCCCTACTTTTCGTATGCATTTAACCAATAAAGAGATTTTAAAC
    AAGCTTTTGAGCTATTCACAAGACTTGAAACACCACTATCAGCTCTATCAACTCTTGCTG
    TTTCACTTTCAGAATAAGGAACCGGAGAAATTTTTCGGACTCATTGAGGACAATCTAAAG
    CAGGTTCATCCTCTTTTTCAGACTGTCTTTAAAACCTTTCTAAAGGACAAAGAGAAAATC
    GTCAACGCCCTTCAACTACACTATTCTAACGCCAAATTGGAAGCGACCAATAATCTCATC
    AAACTTATCAAGCGCAATGCCTTTGGTTTTCGGAACTTTGAAAACTTCAAAAAACGGATT
    TTTATCGCCCTGAATATCAAAAAAGAAAGGACAAAATTTGTCCTTTCTCAAGCTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1257

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPH_2238 [new locus tag: SPH_RS10965 ]
  • symbol: SPH_2238
  • description: transposase
  • length: 418
  • theoretical pI: 10.4494
  • theoretical MW: 49924.3
  • GRAVY: -0.422249

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 12.9)
  • TheSEED  :
    • Mobile element protein
  • ⊞PFAM:
    RNase_H (CL0219) DDE_Tnp_ISL3; Transposase (PF01610; HMM-score: 172.9)
    and 17 more
    Zn_Beta_Ribbon (CL0167) zf-ISL3; zinc-finger of transposase IS204/IS1001/IS1096/IS1165 (PF14690; HMM-score: 45.9)
    HTH (CL0123) HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 36.2)
    Zn_Beta_Ribbon (CL0167) Zn_Tnp_IS1595; Transposase zinc-ribbon domain (PF12760; HMM-score: 19.9)
    HTH (CL0123) HTH_AsnC-type; AsnC-type helix-turn-helix domain (PF13404; HMM-score: 15.3)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 15.1)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 14.1)
    Zn_Beta_Ribbon (CL0167) DZR; Double zinc ribbon (PF12773; HMM-score: 13.7)
    no clan defined TerY_C; TerY-C metal binding domain (PF15616; HMM-score: 13.6)
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 13.4)
    no clan defined DUF6431; Domain of unknown function (DUF6431) (PF20020; HMM-score: 13.4)
    BLYB; Borrelia hemolysin accessory protein (PF05289; HMM-score: 13.3)
    HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 13)
    HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 12.7)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12.2)
    Zn_Beta_Ribbon (CL0167) zf-ribbon_3; zinc-ribbon domain (PF13248; HMM-score: 9.6)
    HTH (CL0123) HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 8.4)
    TRAF (CL0389) zf-ACC; Acetyl-coA carboxylase zinc finger domain (PF17848; HMM-score: 7.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7806
    • Cytoplasmic Membrane Score: 0.0023
    • Cell wall & surface Score: 0.0202
    • Extracellular Score: 0.1969
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025513
    • TAT(Tat/SPI): 0.000328
    • LIPO(Sec/SPII): 0.00107
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACA37024 NCBI
  • UniProt: B1I9D5 UniProt

⊟Protein sequence[edit | edit source]

  • MEQLHFITKLLDIKDTNIQIIDVVNRDSHKEIIAKLDYDAPSCPECGNQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLEGRTQAIIRNHFLKYDRAVRCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFHIVQHLSRAMSRVRVQIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDKHFYRPTFRMHLTNKEILNKLLSYSQDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSQA

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: no polycistronic organisation predicted

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPH_2238&oldid=13156"
  • This page was last edited on 20 January 2022, at 13:33.
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