PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 21-MAR-2025

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⊟Summary

• organism: Streptococcus pneumoniae D39
• locus tag: SPD_RS03135 [old locus tag: SPD_0579 ]
• pan locus tag^?: PNEUPAN001623000
• symbol: SPD_RS03135
• pan gene symbol^?: cbpL
• synonym:
• product: N-acetylmuramoyl-L-alanine amidase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPD_RS03135 [old locus tag: SPD_0579 ]
• symbol: SPD_RS03135
• product: N-acetylmuramoyl-L-alanine amidase family protein
• replicon: chromosome
• strand: +
• coordinates: 606558..607547
• length: 990
• essential: unknown

⊟Accession numbers

• Location: NC_008533 (606558..607547) NCBI
• BioCyc: G1G6V-650 BioCyc
• MicrobesOnline: see SPD_0579
• PneumoBrowse for strain D39V: SPV_0579 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGAATAAACGTCTATTTTTAAAAATGAGTCTGGTGACGTTGCCAATTTTAGCCTTGTTT
  TCACAACCAGTTTTGGCGGAAGAAAACATCCATTTTTCGAGCTGTAAGGAAGCTTGGGCG
  AATGGCTATTCGGATATTCACGAGGGGGAACCTGGTTATTCTGCCAAGTTAGACCGTGAT
  CATGATGGTGTGGCTTGCGAATTGAAAAATGCTCCTAAGGGTGCTTTTAAAGCAAAACAG
  TCAACGGCTATTCAAATCAACACAAGTTCAGCAACAACAAGTGGTTGGGTTAAGCAGGAC
  GGCGCTTGGTACTACTTTGATGGAAATGGAAATCTAGTGAAAAATGCATGGCAGGGAAGC
  TATTACCTGAAAGCTGATGGTAAAATGGCACAGAGTGAATGGATTTATGACTCTTCTTAT
  CAAGCTTGGTATTATTTGAAATCAGATGGTTCTTATGCAAAAAATGCATGGCAAGGAGCT
  TATTACCTTAAATCAAACGGTAAAATGGCACAAGGTGAGTGGGTTTATGATTCTTCTTAC
  CAAGCATGGTATTACTTGAAATCAGATGGTTCATATGCTCGCAATGCATGGCAAGGAAAC
  TACTATTTGAAATCAGATGGTAAAATGGCTAAAGGTGAATGGGTTTATGATGCCACCTAT
  CAAGCTTGGTATTATTTGACATCAGATGGTTCTTATGCTTACAGTACATGGCAAGGAAAT
  TACTATCTAAAATCGGATGGTAAAATGGCTGTCAATGAATGGGTTGATGGTGGACGTTAT
  TATGTTGGCGCTGACGGAGTTTGGAAGGAAGGTCAAGCAAGTACAGCTTCTTCTAGTAAT
  GATAGCAATAGTGAATATTCTGCTGCTTTAGGAAAGGCAAAAAGTTATAATTCGTTATTC
  CACATGTCAAAAAAACGTATGTATAGACAATTAACTTCTGATTTTGATAAATTTTCAAAT
  GATGCAGCTCAATATGCCATTGATCATTAG
  60
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  990
  

⊟Protein

⊟General

• locus tag: SPD_RS03135 [old locus tag: SPD_0579 ]
• symbol: SPD_RS03135
• description: N-acetylmuramoyl-L-alanine amidase family protein
• length: 329
• theoretical pI: 7.56353
• theoretical MW: 37270.8
• GRAVY: -0.729483

⊟Function

• TIGRFAM:
  glucan-binding repeat (TIGR04035; HMM-score: 36.6)
• TheSEED: see SPD_0579
• ⊞PFAM:
  Choline_binding (CL0694) Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 125.3)
  and 6 more
  Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 80.5)
  no clan defined Lipoprotein_Ltp; Host cell surface-exposed lipoprotein (PF07553; HMM-score: 63.1)
  Choline_binding (CL0694) Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 60.9)
  no clan defined Excalibur; Excalibur calcium-binding domain (PF05901; HMM-score: 47.8)
  PmbA_TldD_M; PmbA/TldA metallopeptidase central domain (PF19290; HMM-score: 14.1)
  Transthyretin (CL0287) DUF3823; Protein of unknown function (DUF3823) N-terminal domain (PF12866; HMM-score: 9.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• ⊞SignalP: Signal peptide SP(Sec/SPI) length 26 aa
  • SP(Sec/SPI): 0.997363
  • TAT(Tat/SPI): 0.001338
  • LIPO(Sec/SPII): 0.000744
  • Cleavage Site: CS pos: 26-27. VLA-EE. Pr: 0.9904
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_001042503 NCBI
• UniProt: see SPD_0579

⊟Protein sequence

• MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYSDIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGAWYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGAYYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDATYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTASSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDH

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0579 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Javier Gutiérrez-Fernández, Malek Saleh, Martín Alcorlo, Alejandro Gómez-Mejía, David Pantoja-Uceda, Miguel A Treviño, Franziska Voß, Mohammed R Abdullah, Sergio Galán-Bartual, Jolien Seinen, Pedro A Sánchez-Murcia, Federico Gago, Marta Bruix, Sven Hammerschmidt, Juan A Hermoso
Modular Architecture and Unique Teichoic Acid Recognition Features of Choline-Binding Protein L (CbpL) Contributing to Pneumococcal Pathogenesis.
Sci Rep: 2016, 6;38094
[PubMed:27917891] [WorldCat.org] [DOI] (I e)