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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 22-NOV-2024

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae P1031
  • locus tag: SPP_RS00575 [old locus tag: SPP_0119 ]
  • pan locus tag?: PNEUPAN000679000
  • symbol: purK
  • pan gene symbol?: purK
  • synonym:
  • product: 5-(carboxyamino)imidazole ribonucleotide synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPP_RS00575 [old locus tag: SPP_0119 ]
  • symbol: purK
  • product: 5-(carboxyamino)imidazole ribonucleotide synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 92218..93309
  • length: 1092
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_012467 (92218..93309) NCBI
  • BioCyc: SPP_RS00575 BioCyc
  • MicrobesOnline: see SPP_0119
  • PneumoBrowse for strain D39V: SPV_0060 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGCTCATCTAAAACAATCGGAATTATCGGTGGCGGTCAACTGGGTCAGATGATGGCC
    ATTTCTGCTATCTACATGGGGCACAAGGTTATCGCGCTGGATCCTGCGGCGGATTGCCCG
    GCCTCTCGCGTGGCGGAAATCATTGTGGCACCTTATAACGATGTGGACGCCCTCCGTCAG
    TTGGCAGACCGTTGCGATGTCCTCACTTACGAGTTTGAAAATGTCGACGCTGACGGTTTG
    GATGTCGTTATCAAGGATGGACAACTCCCTCAAGGAACAGATCTGCTCCGCATTTCGCAA
    AATCGTATTTTTGAAAAGGACTTTTTGTCAAACAAGGCTCAAGTCACTGTGGCACCCTAC
    AAGGTCGTGACTTCTAGCCTAGACTTGGCAGATATCGACTTGTCGAAAAACTATGTCCTT
    AAGATCGCAACAGGTGGTTATGATGGACACGGGCAAAATGTCATTCGTTCAGCAGACGAT
    TTGGAAGAAGCCTATGCATTAGCCGACTCAGCAGCTTGTGTCTTGGAAGAATTTGTCAAT
    TTTGACCTTGAGATTTCTGTCATCGTGTCAGGAAATGGGAAGGAGGTGACGTTTTTCCCA
    GTTCAGGAAAATATCCACCGCAACAATATCCTGTCTAAGACCATCGTACCAGCCCGTATT
    TCTGAAAGTCTAGCAGACAAGGCTAAAGCTATGGCAGTGCGAATCGCAGAACAACTCAAC
    TTGTCTGGAACTCTCTGTGTGGAAATGTTTGCGACAGCTGATGACATCATTGTCAATGAG
    ATTGCTCCACGACCACACAACTCAGGCCACTACTCTATTGAAGCCTGTGACTTTTCACAA
    TTTGACACGCATATATTAGGTGTTCTGGGAGCACCATTGCCAGCCATCAAACTACATGCG
    CCTGCCGTCATGCTTAACGTTCTCGGCCAGCATGTTGAGGCCGCTGAAAAATATGTCACA
    GAAAATCCAAGCACCCACCTCCACATGTATGGTAAAATAGAAGCAAAGCATAATCGTAAG
    ATGGGACATGTGACTTTGTTTAGTGATGTACCGGATAGTGTGGAAGAGTTTGGGGAAGGG
    ATTGATTTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1092

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPP_RS00575 [old locus tag: SPP_0119 ]
  • symbol: PurK
  • description: 5-(carboxyamino)imidazole ribonucleotide synthase
  • length: 363
  • theoretical pI: 4.55548
  • theoretical MW: 39368.5
  • GRAVY: 0.0245179

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 6.3.4.18?  ExPASy
    5-(carboxyamino)imidazole ribonucleotide synthase ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
  • ⊞TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 359.3)
    and 8 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 139.2)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylC (TIGR03909; HMM-score: 33.7)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 25.8)
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 23.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 18.8)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 18.4)
    ParB/RepB/Spo0J family partition protein (TIGR00180; HMM-score: 15.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 13.1)
  • TheSEED: see SPP_0119
  • ⊞PFAM:
    ATP-grasp (CL0179) ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 157.9)
    and 9 more
    Hybrid (CL0105) PurK_C; Phosphoribosylaminoimidazole carboxylase C-terminal domain (PF17769; HMM-score: 61.9)
    ATP-grasp (CL0179) ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 25)
    Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 22.5)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 21.8)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 15.3)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 14.8)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 14.7)
    ATP-grasp (CL0179) CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 13.1)
    NADP_Rossmann (CL0063) Rossmann-like; Rossmann-like domain (PF10727; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9837
    • Cytoplasmic Membrane Score: 0.0015
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0148
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.304946
    • TAT(Tat/SPI): 0.024691
    • LIPO(Sec/SPII): 0.009547
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000099033 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRVAEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDVVIKDGQLPQGTDLLRISQNRIFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKIATGGYDGHGQNVIRSADDLEEAYALADSAACVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPARISESLADKAKAMAVRIAEQLNLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPAIKLHAPAVMLNVLGQHVEAAEKYVTENPSTHLHMYGKIEAKHNRKMGHVTLFSDVPDSVEEFGEGIDF

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SPP_RS13280 > purE > purK > SPP_RS00580 > purB

⊟Regulation[edit | edit source]

  • regulator: PurR see SPP_0119

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0060 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPP_RS00575&oldid=122248"
  • This page was last edited on 20 January 2022, at 17:46.
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