⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 31-JAN-2014

⊟Summary

• organism: Streptococcus pneumoniae TIGR4
locus tag: SP_1985 [new locus tag: SP_RS09975 ]
pan locus tag^?: PNEUPAN003567000
symbol: ksgA
pan gene symbol^?: ksgA
• synonym: rsmA
• product: dimethyladenosine transferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SP_1985 [new locus tag: SP_RS09975 ]
• symbol: ksgA
• product: dimethyladenosine transferase
• replicon: chromosome
• strand: -
• coordinates: 1888618..1889457
• length: 840
• essential: unknown

⊟Accession numbers

• Location: AE005672 (1888618..1889457) NCBI
• BioCyc: see SP_RS09975
• MicrobesOnline: 117411 MicrobesOnline
• PneumoBrowse for strain D39V: SPV_1782 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  GTGCTGGAGCGTCACGGTTTTACCTTTAAAAAGTCCTTTGGGCAAAATTTTTTGACGGAT
  ACCAATATCCTTCAAAAAATTGTGGATACGGCTGAAATTGATGATCAGGTCAATGTCATC
  GAAATCGGGCCAGGTATTGGTGCCTTGACAGAATTTTTGGCTGAGCGTGCAGCCCAAGTC
  ATGGCTTTTGAGATTGACCACCGTTTGGTGCCAATTTTGGCAGATACCCTGCGTGATTTT
  GATAATGTGACCGTAGTTAACGAAGATATTCTCAAGGTTGATTTGGCGCAACATATCCAG
  AATTTTAAAAATCCTGACCTGCCAATCAAGGTAGTGGCTAATTTGCCTTACTACATCACG
  ACGCCTATTCTCATGCACTTGATTGAGAGTGGCATTCCTTTTTGTGAGTTTGTGGTCATG
  ATGCAGAAAGAAGTAGCGGACCGCATTTCAGCCCAGCCTAACACCAAGGCTTACGGTAGC
  TTGTCTATCGCCGTGCAGTATTACATGACAGCCAAGGTTGCCTTTATCGTGCCTCGTACG
  GTCTTTGTGCCAGCGCCAAATGTGGATTCAGCCATCTTGAAAATGGTGCGTCGTCCAGAG
  CCAGCCGTAGCAGTAGAAGATGAGAACTTTTTCTTTAAGGTTTCCAAGGCTAGTTTTACC
  CATCGCCGCAAGACCTTGTGGAATAACTTGACAGGTTACTTTGGTAAGACTGAAGAGGTC
  AAGGACAAGCTGACCAAGGCTTTGGACCAGGCAGGCTTGTCACCAAGTGTGCGTGGGGAA
  GCTCTCAGCTTGGCAGAATTTGCCGGTCTAGCAGACGCACTTAAAGGGCAAGGACTCTAA
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⊟Protein

⊟General

• locus tag: SP_1985 [new locus tag: SP_RS09975 ]
• symbol: KsgA
• description: dimethyladenosine transferase
• length: 279
• theoretical pI: 5.70079
• theoretical MW: 31032.6
• GRAVY: 0.0437276

⊟Function

• reaction:
  EC 2.1.1.-?  ExPASy
• ⊞⊞TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 281.1)
  and 9 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 19.9)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 18.6)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 17.3)
  methyltransferase, ATP-grasp peptide maturase system (TIGR04188; HMM-score: 14.4)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 13.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 13.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 12.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 12.5)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 11.9)
• TheSEED  :
  • SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182)
  Protein Metabolism Protein biosynthesis Ribosome biogenesis bacterial  SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182)
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 222.1)
  and 6 more
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 19.8)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 17.5)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 16.7)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 14.1)
  NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 13.4)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001743
  • TAT(Tat/SPI): 0.000125
  • LIPO(Sec/SPII): 0.000177
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: AAK76052 NCBI
• UniProt: Q97NN5 UniProt

⊟Protein sequence

• MLERHGFTFKKSFGQNFLTDTNILQKIVDTAEIDDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRISAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFAGLADALKGQGL

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: cbf1 < ccs50 < SP_1982 < rpe < rsgA < ksgA

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1782 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications