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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 15-DEC-2024

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae 670-6B
  • locus tag: SP670_RS10345 [old locus tag: SP670_2054 ]
  • pan locus tag?: PNEUPAN003593000
  • symbol: pflA
  • pan gene symbol?: pflA
  • synonym:
  • product: pyruvate formate-lyase-activating protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP670_RS10345 [old locus tag: SP670_2054 ]
  • symbol: pflA
  • product: pyruvate formate-lyase-activating protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1918097..1918891
  • length: 795
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_014498 (1918097..1918891) NCBI
  • BioCyc: SP670_RS10345 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1774 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTCTGAAGAAACAATTGATTATGGACAAGTGACAGGAATGGTGCATTCGACAGAAAGC
    TTTGGGTCAGTAGATGGGCCTGGTATTCGCTTTATTGTCTTTTTGCAGGGCTGTCACATG
    CGTTGCCAGTATTGCCACAACCCAGACACTTGGGCTATGGAGTCCAATAAGTCACGTGAA
    CGGACGGTAGATGATGTCTTGACAGAGGCCTTGCGCTACCGTGGTTTCTGGGGAAATAAG
    GGTGGGATTACAGTCAGTGGAGGAGAAGCTCTCTTGCAGATTGATTTCCTGATTGCTCTC
    TTCACCAAGGCTAAGGAACAAGGAATCCACTGTACCTTGGATACCTGTGCTCTTCCTTTC
    CGTAATAAACCACGTTACCTTGAGAAGTTTGACAAACTCATGGCTGTCACTGACTTGGTT
    CTTTTGGATATCAAGGAAATCAACGAAGAACAGCACAAGATTGTCACTAGCCAAACTAAT
    AAAAATATCTTGGCTTGTGCCCAGTATCTATCAGATATTGGAAAACCTGTCTGGATTCGC
    CACGTGCTAGTTCCAGGATTGACAGACAGAGATGATGACTTGATTGAACTTGGTAAGTTC
    GTCAAGACCCTCAAAAATGTTGATAAGTTTGAAATTCTACCTTATCACACCATGGGTGAG
    TTCAAGTGGCGTGAACTTGGAATTCCATATTCCCTCGAAGGAGTCAAACCACCAACAGCA
    GATCGCGTCAAGAACGCTAAAAAACTCATGGATACCGAAAGTTATCAAGATTATATGAAA
    CGTGTACATGGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    795

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP670_RS10345 [old locus tag: SP670_2054 ]
  • symbol: PflA
  • description: pyruvate formate-lyase-activating protein
  • length: 264
  • theoretical pI: 6.71244
  • theoretical MW: 30124.4
  • GRAVY: -0.362879

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 1.97.1.4?  ExPASy
    [Formate-C-acetyltransferase]-activating enzyme S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical
  • ⊞TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair pyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 310.4)
    Metabolism Energy metabolism Anaerobic pyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 310.4)
    and 25 more
    glycyl-radical enzyme activating protein (TIGR02494; EC 1.97.1.-; HMM-score: 185.2)
    Genetic information processing Protein fate Protein modification and repair glycine radical enzyme activase, YjjW family (TIGR04041; EC 1.97.1.-; HMM-score: 148.7)
    Metabolism Energy metabolism Amino acids and amines choline TMA-lyase-activating enzyme (TIGR04395; EC 1.97.-.-; HMM-score: 134.2)
    Genetic information processing Protein fate Protein modification and repair [benzylsuccinate synthase]-activating enzyme (TIGR04003; EC 1.97.-.-; HMM-score: 117.2)
    Genetic information processing Protein fate Protein modification and repair anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 69.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 69.2)
    Genetic information processing Protein fate Protein modification and repair anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02491; EC 1.97.1.-; HMM-score: 56.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02491; EC 1.97.1.-; HMM-score: 56.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02826; EC 1.97.1.-; HMM-score: 29.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR03963; HMM-score: 28.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other coenzyme PQQ biosynthesis enzyme PqqE (TIGR02109; HMM-score: 27)
    putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR04349; HMM-score: 27)
    AmmeMemoRadiSam system radical SAM enzyme (TIGR04337; HMM-score: 24.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR03365; HMM-score: 24.1)
    His-Xaa-Ser system radical SAM maturase HxsC (TIGR03977; HMM-score: 21.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin probable molybdenum cofactor biosynthesis protein A (TIGR02668; HMM-score: 19.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor biosynthesis protein A (TIGR02666; HMM-score: 19.1)
    putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR04322; HMM-score: 16.9)
    radical SAM protein, TatD family-associated (TIGR04038; HMM-score: 15.8)
    Signal transduction Regulatory functions Protein interactions peptide modification radical SAM enzyme, YydG family (TIGR04078; HMM-score: 14.5)
    His-Xaa-Ser system radical SAM maturase HxsB (TIGR03978; HMM-score: 13.7)
    putative peptide-modifying radical SAM enzyme, Mhun_1560 family (TIGR04083; HMM-score: 13.3)
    putative geopeptide radical SAM maturase (TIGR04280; HMM-score: 12.7)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein S12 methylthiotransferase RimO (TIGR01125; EC 2.1.1.-,2.8.1.-; HMM-score: 12.4)
    Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_1487 family (TIGR04013; HMM-score: 10.6)
  • TheSEED: see SP670_2054
  • ⊞PFAM:
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 107.9)
    and 2 more
    4Fe-4S (CL0344) Fer4_12; 4Fe-4S single cluster domain (PF13353; HMM-score: 63.3)
    Fer4_14; 4Fe-4S single cluster domain (PF13394; HMM-score: 27.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.982
    • Cytoplasmic Membrane Score: 0.0089
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0091
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.049982
    • TAT(Tat/SPI): 0.001379
    • LIPO(Sec/SPII): 0.020704
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001288288 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQYCHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFTKAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTNKNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTMGEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for TIGR4

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_1774 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SP670_RS10345&oldid=19668"
  • This page was last edited on 20 January 2022, at 13:48.
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