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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_0317 [new locus tag: SP_RS01540 ]
  • pan locus tag?: PNEUPAN001123000
  • symbol: SP_0317
  • pan gene symbol?: eda
  • synonym:
  • product: 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxyphosphogluconate aldolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_0317 [new locus tag: SP_RS01540 ]
  • symbol: SP_0317
  • product: 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxyphosphogluconate aldolase
  • replicon: chromosome
  • strand: -
  • coordinates: 291831..292460
  • length: 630
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: AE005672 (291831..292460) NCBI
  • BioCyc: see SP_RS01540
  • MicrobesOnline: 115852 MicrobesOnline
  • PneumoBrowse for strain D39V: SPV_0289 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACCAAATCAGATACGATTATTGAACTAAAAAAACAAAAAATTGTCGCTGTTATTCGA
    GGAAATACAAAGGAAGAAGGACTACAAGCCTCGATTGCTTGTATCAAGGGCGGTATCAAA
    GCTATTGAAATCGCCTATACCAATCAGTATGCAGGACAAATCATCAAGGAACTTGTAGAC
    TTGTATCAGGACGATCAGAGTGTTTGTATCGGTGCAGGTACTGTGCTTGATGCCGTAACT
    GCTAGAGATGCCATTCTAGCTGGAGCAAATTACGTTGTTTCTCCATCTTTCCATGCTGAA
    ACTGCGAAAATGTGCAATCTCTACAGCACACCGTACATTCCAGGCTGTATTACCCTCACA
    GAGATCACGACTGCACTTGAAGCCGGTAGTGAAATCATCAAACTCTTCCCAGGTAGTACT
    CTCAGTCCAGCATATATCTCTGCAGTCAAGGCACCGATCCCACAAGTTTCCGTAATGGTA
    ACCGGAGGAGTCGGCCTAAACAACATCCCTCAATGGTTCGCTGCTGGTGCAGATGCCGTT
    GGAATTGGTGGCGAACTCAATAAACTCGCTTCCCAAGGCAACTTTGACCGCATCAGCGAG
    ATTGCCCAACAGTATATTACACTCAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    630

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_0317 [new locus tag: SP_RS01540 ]
  • symbol: SP_0317
  • description: 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxyphosphogluconate aldolase
  • length: 209
  • theoretical pI: 4.95702
  • theoretical MW: 22068.3
  • GRAVY: 0.222967

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 4.1.3.16?  ExPASy
    4-hydroxy-2-oxoglutarate aldolase 4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate
  • ⊞TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase (TIGR01182; EC 4.1.2.14,4.1.3.16; HMM-score: 161)
    Metabolism Energy metabolism Entner-Doudoroff 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase (TIGR01182; EC 4.1.2.14,4.1.3.16; HMM-score: 161)
    and 4 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotate dehydrogenase (fumarate) (TIGR01036; EC 1.3.98.1; HMM-score: 15.7)
    Metabolism Energy metabolism Pentose phosphate pathway ribulose-phosphate 3-epimerase (TIGR01163; EC 5.1.3.1; HMM-score: 13.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 13.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides nicotinate-nucleotide diphosphorylase (carboxylating) (TIGR00078; EC 2.4.2.19; HMM-score: 13)
  • ⊞TheSEED  :
    • 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14)
    • 4-Hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16)
    Carbohydrates Central carbohydrate metabolism Entner-Doudoroff Pathway  2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14)
    and 2 more
    Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  4-Hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16)
    Carbohydrates One-carbon Metabolism Formaldehyde assimilation: Ribulose monophosphate pathway  2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14)
  • ⊞PFAM:
    TIM_barrel (CL0036) Aldolase; KDPG and KHG aldolase (PF01081; HMM-score: 129.5)
    and 3 more
    QRPTase_C; Quinolinate phosphoribosyl transferase, C-terminal domain (PF01729; HMM-score: 15.8)
    Ribul_P_3_epim; Ribulose-phosphate 3 epimerase family (PF00834; HMM-score: 15.1)
    DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.020047
    • TAT(Tat/SPI): 0.000385
    • LIPO(Sec/SPII): 0.004331
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: AAK74493 NCBI
  • UniProt: A0A0H2UNC8 UniProt

⊟Protein sequence[edit | edit source]

  • MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEIAYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETAKMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMVTGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SP_0316 < SP_0317 < SP_0318 < SP_0319 < SP_0320

⊟Regulation[edit | edit source]

  • ⊞regulator: RegR (repression) regulon
    RegR(TF)important in Hyaluronate utilization; RegPrecise 

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0289 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SP_0317&oldid=2537"
  • This page was last edited on 20 January 2022, at 13:10.
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