⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 05-OCT-2017

⊟Summary

• organism: Streptococcus pneumoniae MDRSPN001
locus tag: MDRSPN_00976 [new locus tag: MDRSPN_RS05095 ]
pan locus tag^?: PNEUPAN002277000
symbol: eno
pan gene symbol^?: eno
• synonym:
• product: enolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: MDRSPN_00976 [new locus tag: MDRSPN_RS05095 ]
• symbol: eno
• product: enolase
• replicon: chromosome
• strand: +
• coordinates: 983383..984687
• length: 1305
• essential: unknown other strains

⊟Accession numbers

• Location: AP018391 (983383..984687) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1012 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGTCAATTATTACTGATGTTTACGCTCGCGAAGTCCTAGACTCACGCGGTAACCCAACA
  CTTGAGGTAGAAGTTTATACTGAATCAGGTGCTTTCGGACGTGGTATGGTTCCATCAGGA
  GCTTCTACTGGTGAACACGAAGCAGTTGAACTTCGCGACGGTGACAAATCTCGTTACGGT
  GGTCTTGGTACACAAAAAGCTGTTGACAACGTAAACAACATCATCGCTGAAGCTATCATC
  GGCTACGATGTACGTGACCAACAAGCTATCGACCGTGCTATGATCGCACTTGACGGTACT
  CCTAACAAAGGTAAATTGGGTGCAAACGCAATTCTTGGTGTGTCTATCGCTGTAGCTCGT
  GCTGCTGCTGACTACCTTGAAATCCCACTTTACAGCTACCTTGGTGGATTCAACACTAAA
  GTTCTTCCAACTCCAATGATGAACATCATCAACGGTGGTTCTCACTCTGACGCTCCAATC
  GCTTTCCAAGAATTCATGATCGTACCTGCTGGTGCACCAACATTCAAAGAAGCTCTTCGT
  TGGGGTGCTGAAATCTTCCACGCTCTTAAGAAAATTCTTAAATCACGTGGTTTGGAAACA
  GCCGTAGGTGACGAAGGTGGATTCGCTCCTCGTTTCGAAGGAACTGAAGACGGTGTTGAA
  ACTATCCTTGCTGCAATCGAAGCTGCTGGATATGTTCCTGGTAAAGACGTATTTATCGGA
  TTTGACTGTGCTTCATCAGAATTCTATGATGAAGAACGTAAAGTTTACGACTACACTAAA
  TTTGAAGGTGAAGGCGCTGCTGTACGTACTGCTGCAGAACAAATCGACTACCTTGAAGAA
  TTGGTAAACAAATACCCAATCATCACTATCGAAGATGGTATGGATGAAAACGACTGGGAC
  GGTTGGAAAGCTCTTACTGAACGTCTTGGTGGTAAAGTACAATTGGTTGGTGATGACTTC
  TTCGTAACAAACACTTCTTACCTTGAAAAAGGTATTGCAGAAGGTGCTGCTAACTCAATC
  CTTATCAAAGTTAACCAAATCGGTACTCTTACTGAAACATTCGACGCTATCGAAATGGCG
  AAAGAAGCTGGTTACACTGCAGTTGTATCACACCGTTCAGGTGAAACTGAAGATTCAACA
  ATCGCTGACATCGCAGTTGCAACTAACGCAGGACAAATCAAGACTGGTTCACTTTCACGT
  ACAGACCGCATCGCTAAATACAACCAATTGCTTCGTATCGAAGACCAACTTGGTGAAGTA
  GCTGAATATCGTGGATTGAAATCATTCTACAACCTTAAAAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1305
  

⊟Protein

⊟General

• locus tag: MDRSPN_00976 [new locus tag: MDRSPN_RS05095 ]
• symbol: Eno
• description: enolase
• length: 434
• theoretical pI: 4.34278
• theoretical MW: 46928.3
• GRAVY: -0.194931

⊟Function

• ⊞⊞TIGRFAM:
  Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphopyruvate hydratase (TIGR01060; EC 4.2.1.11; HMM-score: 687.8)
  and 4 more
  Metabolism Energy metabolism Fermentation methylaspartate ammonia-lyase (TIGR01502; EC 4.3.1.2; HMM-score: 22.9)
  Metabolism Energy metabolism Amino acids and amines methylaspartate ammonia-lyase (TIGR01502; EC 4.3.1.2; HMM-score: 22.9)
  muconate and chloromuconate cycloisomerases (TIGR02534; EC 5.5.1.-; HMM-score: 19.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone o-succinylbenzoate synthase (TIGR01928; EC 4.2.1.113; HMM-score: 18.6)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• ⊞⊞PFAM:
  Enolase_TIM (CL0256) Enolase_C; Enolase, C-terminal TIM barrel domain (PF00113; HMM-score: 432.5)
  and 3 more
  Enolase_N (CL0227) Enolase_N; Enolase, N-terminal domain (PF03952; HMM-score: 204.6)
  Enolase_TIM (CL0256) MAAL_C; Methylaspartate ammonia-lyase C-terminus (PF07476; HMM-score: 31.5)
  MR_MLE_C; Enolase C-terminal domain-like (PF13378; HMM-score: 28.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006799
  • TAT(Tat/SPI): 0.001562
  • LIPO(Sec/SPII): 0.001052
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: BBA59179 NCBI
• UniProt:

⊟Protein sequence

• MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTGEHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPNKGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPIAFQEFMIVPAGAPTFKEALRWGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDGVETILAAIEAAGYVPGKDVFIGFDCASSEFYDEERKVYDYTKFEGEGAAVRTAAEQIDYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGKVQLVGDDFFVTNTSYLEKGIAEGAANSILIKVNQIGTLTETFDAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_1012 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

S Bergmann, M Rohde, G S Chhatwal, S Hammerschmidt
alpha-Enolase of Streptococcus pneumoniae is a plasmin(ogen)-binding protein displayed on the bacterial cell surface.
Mol Microbiol: 2001, 40(6);1273-87
[PubMed:11442827] [WorldCat.org] [DOI] (P p)Simone Bergmann, Daniela Wild, Oliver Diekmann, Ronald Frank, Dagmar Bracht, Gursharan S Chhatwal, Sven Hammerschmidt
Identification of a novel plasmin(ogen)-binding motif in surface displayed alpha-enolase of Streptococcus pneumoniae.
Mol Microbiol: 2003, 49(2);411-23
[PubMed:12828639] [WorldCat.org] [DOI] (P p)Stefanie Ehinger, Wolf-Dieter Schubert, Simone Bergmann, Sven Hammerschmidt, Dirk W Heinz
Plasmin(ogen)-binding alpha-enolase from Streptococcus pneumoniae: crystal structure and evaluation of plasmin(ogen)-binding sites.
J Mol Biol: 2004, 343(4);997-1005
[PubMed:15476816] [WorldCat.org] [DOI] (P p)Simone Bergmann, Manfred Rohde, Klaus T Preissner, Sven Hammerschmidt
The nine residue plasminogen-binding motif of the pneumococcal enolase is the major cofactor of plasmin-mediated degradation of extracellular matrix, dissolution of fibrin and transmigration.
Thromb Haemost: 2005, 94(2);304-11
[PubMed:16113819] [WorldCat.org] [DOI] (P p)Jan Kolberg, Audun Aase, Simone Bergmann, Tove K Herstad, Gunnhild Rødal, Ronald Frank, Manfred Rohde, Sven Hammerschmidt
Streptococcus pneumoniae enolase is important for plasminogen binding despite low abundance of enolase protein on the bacterial cell surface.
Microbiology (Reading): 2006, 152(Pt 5);1307-1317
[PubMed:16622048] [WorldCat.org] [DOI] (P p)Yuka Mori, Masaya Yamaguchi, Yutaka Terao, Shigeyuki Hamada, Takashi Ooshima, Shigetada Kawabata
α-Enolase of Streptococcus pneumoniae induces formation of neutrophil extracellular traps.
J Biol Chem: 2012, 287(13);10472-10481
[PubMed:22262863] [WorldCat.org] [DOI] (I p)Vaibhav Agarwal, Sven Hammerschmidt, Sven Malm, Simone Bergmann, Kristian Riesbeck, Anna M Blom
Enolase of Streptococcus pneumoniae binds human complement inhibitor C4b-binding protein and contributes to complement evasion.
J Immunol: 2012, 189(7);3575-84
[PubMed:22925928] [WorldCat.org] [DOI] (I p)Simone Bergmann, Hanne Schoenen, Sven Hammerschmidt
The interaction between bacterial enolase and plasminogen promotes adherence of Streptococcus pneumoniae to epithelial and endothelial cells.
Int J Med Microbiol: 2013, 303(8);452-62
[PubMed:23906818] [WorldCat.org] [DOI] (I p)