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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 19-NOV-2015

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae A66
  • locus tag: A66_01095 [new locus tag: A66_RS05490 ]
  • pan locus tag?: PNEUPAN002454000
  • symbol: A66_01095
  • pan gene symbol?: cysD
  • synonym:
  • product: O-acetylhomoserine sulfhydrylase / O-succinylhomoserine sulfhydrylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: A66_01095 [new locus tag: A66_RS05490 ]
  • symbol: A66_01095
  • product: O-acetylhomoserine sulfhydrylase / O-succinylhomoserine sulfhydrylase
  • replicon: chromosome
  • strand: -
  • coordinates: 1060401..1061525
  • length: 1125
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: LN847353 (1060401..1061525) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_2310 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTTTGCCTTGAGGAAACCAGGGAACATTTATACTCGTATCACCAATCCTACAACAGCT
    GCCCTTGAAGGTGGTGTTGAAGCGCTAGCAACAGCATCAGGTATGACTGCAGTGACTTAT
    ACTATTTTGGCGATTGCCCATGCTGGTGACCATGTAGTGGCTGCTTCGACTATTTACGGT
    GGAACCTTCAATCTTTTGAAAAAACCCCTTCCTCGTTATGGTATCACAACAACCTTTGTC
    GATATTGATAATTTGGAGGAAGTAGAAGCAGCTATCAAAGACAATACCAAGCTTGTCTTG
    ATTGAAACCTTGGGTAACCCCTTGATTAATATTCCAGACCTGGAAAAACTGGCAGAGATT
    GCTCATAAACATCAAATCCCACTTGTGTCAGACAATACTTTTGCAACACCTTATTTGATT
    AACGTCTTCTCTCATGGCGTTGACATTGCCATTCACTCTGCGACTAAGTTTATCGGTGGG
    CATGGTACAACTATTGGAGGAATAATTGTCGATAGTGGTCGTTTTGACTGGACGGCTTCA
    GGGAAATTCCCTCAATTTGTTGACGAGGGTCCAAGCTGCCACAATTTGAGTTATACTCGT
    GATGTGGGTGCAGCAGCCTTTATTATAGCTGTTCGAGTTCAATTGCTTCGTGATACAGGT
    GCAGCCTTGTCACCATTCAATGCTTTCCTCTTGCTACAAAGACTTGAAACCCTTTCACTT
    CGTGTGGAACGCCATGTACAAAATGCTGAGACAATTGTTGATTTTCTTGTCAACCATCCT
    AAGGTAGAAAAGGTAAATTATCCAAAACTTGCAGATAGTCCTTATTATGCCTTGGCTGAG
    AAATACTTGCCAAAAGGTGTCGGTTCAATCTTTACCTTCCACGTCAAAGGTGACGAGGAA
    GAAGCACGCAAGGTCATTGATAATTTAGAAATCTTTTCTGACCTTGCAAACGCGGCAGAT
    GCTAAATCGCTTGTTGTCCATCCAGCAACAATCACTCACGGTCAATTGTCAGAAAAAGAC
    CTAGAAGCAGCAGGTGTCACACCAAATCAAATCCACTTGTCTATCGGTCTTGAAAATGTA
    GAAGATTTGATTGAAGACTTACGCTTGGCCTTGGAAAAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1125

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: A66_01095 [new locus tag: A66_RS05490 ]
  • symbol: A66_01095
  • description: O-acetylhomoserine sulfhydrylase / O-succinylhomoserine sulfhydrylase
  • length: 374
  • theoretical pI: 5.52915
  • theoretical MW: 40565
  • GRAVY: 0.0874332

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.48?  ExPASy
    Cystathionine gamma-synthase O4-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate
    EC 2.5.1.49?  ExPASy
    O-acetylhomoserine aminocarboxypropyltransferase O-acetyl-L-homoserine + methanethiol = L-methionine + acetate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 523.3)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 523.3)
    and 21 more
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 328.2)
    Metabolism Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 320.1)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 247.9)
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 204.6)
    Metabolism Amino acid biosynthesis Aspartate family cystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 151.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 25.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 25.1)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 23.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 23.1)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01976; HMM-score: 23)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 21.8)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 19.7)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 19.6)
    Unknown function Enzymes of unknown specificity uncharacterized pyridoxal phosphate-dependent enzyme (TIGR01437; HMM-score: 19.4)
    O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 17.7)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 17.1)
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 17)
    Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 15.4)
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase (TIGR03588; EC 2.6.1.92; HMM-score: 12.2)
    Cellular processes Cellular processes Biosynthesis of natural products fluorothreonine transaldolase (TIGR04506; EC 2.2.1.8; HMM-score: 12.1)
    aminotransferase, LLPSF_NHT_00031 family (TIGR04181; HMM-score: 11)
  • TheSEED: data available for D39
  • PFAM:
    PLP_aminotran (CL0061) Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 390.5)
    and 5 more
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 37.4)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 33.4)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 31.7)
    SepSecS; O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS (PF05889; HMM-score: 15.5)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9098
    • Cytoplasmic Membrane Score: 0.0141
    • Cell wall & surface Score: 0.0021
    • Extracellular Score: 0.074
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.125187
    • TAT(Tat/SPI): 0.04442
    • LIPO(Sec/SPII): 0.00718
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CRI61806 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MFALRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAHAGDHVVAASTIYGGTFNLLKKPLPRYGITTTFVDIDNLEEVEAAIKDNTKLVLIETLGNPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSATKFIGGHGTTIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGAALSPFNAFLLLQRLETLSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYYALAEKYLPKGVGSIFTFHVKGDEEEARKVIDNLEIFSDLANAADAKSLVVHPATITHGQLSEKDLEAAGVTPNQIHLSIGLENVEDLIEDLRLALEKI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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