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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 19-NOV-2015

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae A66
  • locus tag: A66_01708 [new locus tag: A66_RS08575 ]
  • pan locus tag?: PNEUPAN003434000
  • symbol: A66_01708
  • pan gene symbol?: rumA-2
  • synonym: rlmD
  • product: RNA methyltransferase, TrmA family

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: A66_01708 [new locus tag: A66_RS08575 ]
  • symbol: A66_01708
  • product: RNA methyltransferase, TrmA family
  • replicon: chromosome
  • strand: -
  • coordinates: 1634167..1635270
  • length: 1104
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: LN847353 (1634167..1635270) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1704 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCACCTGCATTATGATAAGCAGCTGGAGTTCAAGACGGACTTACTTCATCAAGCGCTG
    AAAAAATTTGCTCCTGCAGGATATGAAAATTATGAAATTCGTCCAACTATTGGAATGCAG
    GAACCAAAATATTACAGAGCTAAGTTACAATTTCAGACTCGAAAATTTAAAAATCAGGTC
    AAGGCGGGCTTATATGCACAAAACTCTCACTATTTAGTAGAGTTGAAAGACTGCCTGGTA
    CAAGATAAGGAAACCCAAGTGATTGCTAATCGCTTAGCAGAATTACTTACTTATCACCAG
    ATTCCAATCACGGATGAGAGAAAAGTTCTAGGTGTCCGTACTATTATGGTCCGACGCGCG
    AGAAAGACCGGACAGGTTCAGATTATTATTGTTACAAACCGCCAGCTTAATTTAACTCAA
    TTGGTAAAAGAGTTGGTTAAAGATTTCCCAGAAGTTGTGACAGTAGCTGTTAATACAAAT
    ACAGCTAAAACCAGTGAGATATATGGTGAAAAGACAGAGATTATCTGGGGGCAAGAGAGT
    ATTCAAGAAGGTGTACTCAATTATGAATTTTCACTATCCCCTCGAGCTTTTTATCAACTA
    AATCCTGAGCAAACAGAAGTCCTCTATAGCGAAGCAGTAAAAGCGCTGGATGTTGATAAA
    GAAGACCATTTGATTGACGCTTATTGTGGAGTTGGAACGATTGGATTTGCCTTTGCAAAG
    AAAGTAAAAACACTCAGAGGTATGGATATTATTCCAGAAGCTATTGAAGATGCCAAGCGA
    AATGCTAAAAGAATGGGATTTGACAATACTCATTATGAAGCTGGAACGGCAGAAGAGATT
    ATTCCTCGTTGGTACAAGGAAGGCTACCGAGCAGATGCTTTGATTGTTGACCCACCACGT
    ACAGGTCTGGATGATAAGTTATTAGATACTATTCTTACTTATGTACCAGAAAAAATGGTT
    TATATTTCTTGTAATGTTTCGACCTTGGCTCGTGATTTGGTACGCTTAGTAGAAGTCTAT
    GATCTTCATTATATCCAGTCGGTCGATATGTTCCCACATACAGCTCGAACTGAAGCTGTT
    GTAAAATTAATAAAAAAAGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1104

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: A66_01708 [new locus tag: A66_RS08575 ]
  • symbol: A66_01708
  • description: RNA methyltransferase, TrmA family
  • length: 367
  • theoretical pI: 7.66722
  • theoretical MW: 42251.4
  • GRAVY: -0.335422

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 345.8)
    and 12 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumB (TIGR02085; EC 2.1.1.189; HMM-score: 133.2)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (uracil(54)-C(5))-methyltransferase (TIGR02143; EC 2.1.1.35; HMM-score: 75.1)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 33.9)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 25.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 24.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 23.2)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 21.5)
    Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 20.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 20.6)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 20.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 18.5)
    Cellular processes Cellular processes Sporulation and germination stage III sporulation protein AA (TIGR02858; HMM-score: 13)
  • TheSEED: data available for D39, TIGR4
  • PFAM:
    NADP_Rossmann (CL0063) tRNA_U5-meth_tr; tRNA (Uracil-5-)-methyltransferase (PF05958; HMM-score: 74.5)
    and 10 more
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 40.6)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 28.6)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 24)
    Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 18.6)
    Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 17.8)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 17.7)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 15.7)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 15.5)
    Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 14.1)
    no clan defined PriA_C; Primosomal protein N C-terminal domain (PF18074; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009905
    • TAT(Tat/SPI): 0.001854
    • LIPO(Sec/SPII): 0.003863
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CRI62419 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELKDCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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