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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 13-DEC-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae A66
  • locus tag: A66_RS03575 [old locus tag: A66_00720 ]
  • pan locus tag?: PNEUPAN001758000
  • symbol: metG
  • pan gene symbol?: metG
  • synonym:
  • product: methionine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: A66_RS03575 [old locus tag: A66_00720 ]
  • symbol: metG
  • product: methionine--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 687768..689765
  • length: 1998
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_LN847353 (687768..689765) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0689 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCTGAAAAAAATTTTTATATTACAACGCCGATTTACTATCCATCTGGGAAACTTCAT
    ATCGGTTCTGCCTACACAACTATCGCATGTGATGTCCTAGCACGTTACAAACGTCTGATG
    GGCTACGATGTATTTTATCTGACAGGTCTTGATGAACATGGTCAGAAAATCCAGCAGAAA
    GCGGAAGAAGCTGGTATTACACCTCAAGCCTATGTTGATGGAATGGCGGTTGGAGTTAAA
    GAACTCTGGCAATTACTAGATATCTCATACGATAAATTTATCCGTACAACTGATGACTAC
    CATGAAAAAGTTGTCGCACAGGTCTTTGAACGCTTACTTGCCCAAGATGATATCTACTTG
    GGTGAATACTCTGGTTGGTATTCAGTATCAGACGAAGAATTCTTTACAGAAAGCCAGCTG
    GCAGAAGTTTTCCGTGATGAAGCTGGAAATGTGACTGGCGGTATTGCTCCATCAGGTCAT
    GAGGTTGAATGGGTTTCTGAAGAATCATACTTCCTTCGCCTTAGCAAATACCAAGACCGT
    TTGGTCGAATTTTTCAAAGCTCATCCTGAATTTATCACGCCAGATGGTCGCCTTAATGAA
    ATGCTACGCAACTTCATCGAGCCAGGTTTGGAAGATTTGGCGGTATCTCGTACAACCTTT
    ACATGGGGAGTGCCTGTCCCATCAAATCCAAAACACGTTGTCTACGTTTGGATTGATGCC
    CTTCTTAACTATGCGACAGCTCTTGGCTACGCTCAAGACGAACATGGTAACTTTGACAAG
    TTCTGGAATGGAACAGTCTTCCATATGGTAGGAAAAGATATCCTTCGCTTCCACTCTATC
    TACTGGCCAATCCTTCTTATGATGTTGGATGTTAAATTACCTGATCGTTTGATTGCCCAT
    GGTTGGTTTGTCATGAAAGACGGAAAAATGTCTAAGTCAAAAGGGAATGTCGTTTACCCT
    GAAATGTTGGTAGAGCGTTATGGACTAGATCCACTTCGTTACTACCTCATGCGTAACCTT
    CCAGTTGGTTCAGACGGAACCTTTACTCCTGAAGACTATGTCGGCCGTATCAACTATGAA
    TTGGCTAATGACCTTGGGAACCTCCTTAACCGTACGGTTTTCATGATTAATAAGTACTTT
    GATGGACAAATCCCTGCCTATGTAGAAGGTGTGACTGAATTTGACCATGTTCTTGCTGAG
    GTTGCAGAACAATCAATCGCAGACTTCCATGCACACATGGAAGCAGTTGACTATCCACGT
    GCGCTTGAAGCAGTCTGGACTCTGATCTCTCGTACCAATAAATACATCGATGAGACCGCA
    CCATGGGTCTTGGCCAAGGATGAAGCTCTTCGTGACCAATTGGCAAGTGTCATGAGCCAC
    TTGGCAGCCAGCATTCGTGTAGTTGCTCACTTGATTGAACCATTTATGATGGAAACTAGT
    CGTATAGTTTTGACTCAACTTGGTTTGGAAGAAGTTTCTAGTCTTGAAAACTTGAGTTTG
    GCTGACTTCCCAGCAGATGTGACTGTAGTTGCCAAAGGAACACCTATCTTTCCACGTCTA
    AATATGGAAGAAGAAATCGCCTATATCAAGGAACAAATGGAAGGCAATAAACCAGCAGTC
    GAAAAAGAATGGAATCCGGACGAAGTTGAGCTCAAACTAAACAAGGATGAAATCAAGTTT
    GAAGACTTTGACAAGGTTGAAATCCGTGTTGCAGAAGTCAAAGAAGTGTCTAAAGTAGAA
    GGTTCAGATAAGTTACTTCAATTCCGCTTGGATGCTGGTGATGGAGAAGATCGTCAGATT
    CTTTCAGGAATTGCAAAATACTATCCAAATGAACAAGAATTGGTCGGCAAGAAAGTTCAA
    ATCGTTGCTAACCTCAAACCACGTAAAATGATGAAAAAATATGTCAGCCAGGGTATGATT
    CTCTCAGCTGAACATGATGGCAAATTAACCCTTCTCACAGTTGATCCAGCTGTACCAAAC
    GGAAGTGTGATTGGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    1998

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: A66_RS03575 [old locus tag: A66_00720 ]
  • symbol: MetG
  • description: methionine--tRNA ligase
  • length: 665
  • theoretical pI: 4.61395
  • theoretical MW: 75722.8
  • GRAVY: -0.226165

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.10?  ExPASy
    Methionine--tRNA ligase ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 555.5)
    and 12 more
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase, beta subunit (TIGR00399; EC 6.1.1.10; HMM-score: 124.4)
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 115.6)
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 88.7)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 86.9)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 57.1)
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 48.5)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking export-related chaperone protein CsaA (TIGR02222; HMM-score: 28.6)
    Genetic information processing Protein fate Protein folding and stabilization export-related chaperone protein CsaA (TIGR02222; HMM-score: 28.6)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, beta subunit (TIGR00472; EC 6.1.1.20; HMM-score: 15.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 14.3)
    Genetic information processing Protein synthesis tRNA aminoacylation arginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 11.6)
    Genetic information processing Protein synthesis tRNA aminoacylation glutamate--tRNA ligase (TIGR00464; EC 6.1.1.17; HMM-score: 10.5)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 366.2)
    and 6 more
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 86.6)
    OB (CL0021) tRNA_bind; Putative tRNA binding domain (PF01588; HMM-score: 79.4)
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 43.3)
    DALR (CL0258) Anticodon_3; Anticodon binding domain of methionyl tRNA ligase (PF19303; HMM-score: 36.6)
    HUP (CL0039) tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 26)
    DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA ligase (PF08264; HMM-score: 19.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.040335
    • TAT(Tat/SPI): 0.000847
    • LIPO(Sec/SPII): 0.029373
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_053039532 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDVFYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHEKVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGHEVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRTTFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRYYLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVFMINKYFDGQIPAYVEGVTEFDHVLAEVAEQSIADFHAHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEALRDQLASVMSHLAASIRVVAHLIEPFMMETSRIVLTQLGLEEVSSLENLSLADFPADVTVVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKVEIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVANLKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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