PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 17-DEC-2024

⊟Summary

• organism: Streptococcus pneumoniae A66
• locus tag: A66_RS05665 [old locus tag: A66_01135 ]
• pan locus tag^?: PNEUPAN002126000
• symbol: A66_RS05665
• pan gene symbol^?: mutR2
• synonym:
• product: helix-turn-helix domain-containing protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: A66_RS05665 [old locus tag: A66_01135 ]
• symbol: A66_RS05665
• product: helix-turn-helix domain-containing protein
• replicon: chromosome
• strand: +
• coordinates: 1103301..1104152
• length: 852
• essential: unknown

⊟Accession numbers

• Location: NZ_LN847353 (1103301..1104152) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0939 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  
  ATGAAATCAAAACTTGGCATCACACTTAGAAAAGTTCGTAAAGGAAAACAAATTAGTCTA
  TACTCTGTAGCAGATGAACATCTTTCAAAATCTCAAATATCTAGATTCGAACGTGGGGAA
  TCAGAGATATCTTGTATTCGACTTATCAATATTTTAGACAAATTACACATTACTTTAGAT
  GAGTTTCTTGTTTTACATGATGAAGATTATACTAATACTGAATTATTCGCTAATTTGGTT
  CAATATATTCGTAAGCAATATTCATCACAAAACATCAAAAATATAGCCGCTCTACTTTCT
  GACTCATCACATTGCACTTTAAATTCTTTCGAGAAAACAATGGTAAAATCCATTCTACAT
  ACAATGGATTCAACTATTTTTCCATCAGATGCAGAATTGCTTCAATTGACAGATTATCTC
  TTCAAAGTTGAAAAATGGGGCTACTATGAAATCACTTTATTAGGTAACTGTGTCCGAACA
  ATAAACTACAACTCCTACTTCCTATTAACAAAGGAAATGTTGAACAATTACATCTACTCT
  TCATTGAATAAAACAAATAAGCGAATTGTCACTCAATTGGCCATCAATTGTCTAATTCTG
  AGCGTAGATAAGGAAGAATTTTCAAACTGTACTTTTCTAATCACCGAAATTAAAGCATTG
  TTAGATAATGAATTAAACTTTTATGAACAGACAGTTTTTCTCTATACAACTGGTTACTTT
  GAATTTAAGTGTCATTCAGATAGCGGAATTGAAAAAATGAAACAGGCCATACAAGTGCTT
  GATATTTTAGGAGAAGACAAGTTAAAATTACACTATACTAGCCATTTCGATAAACTAGTA
  AACAAAGAATAG
  60
  120
  180
  240
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  360
  420
  480
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  780
  840
  852
  

⊟Protein

⊟General

• locus tag: A66_RS05665 [old locus tag: A66_01135 ]
• symbol: A66_RS05665
• description: helix-turn-helix domain-containing protein
• length: 283
• theoretical pI: 6.7121
• theoretical MW: 32950.7
• GRAVY: -0.203534

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions transcriptional activator, Rgg/GadR/MutR family, C-terminal domain (TIGR01716; HMM-score: 192.4)
  and 2 more
  TQXA domain (TIGR03934; HMM-score: 13.4)
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 12.3)
• TheSEED: data available for D39
• PFAM:
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 24.6)
  and 4 more
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 15.8)
  no clan defined EIF_2_alpha; Eukaryotic translation initiation factor 2 alpha subunit (PF07541; HMM-score: 14.7)
  NADH_4Fe-4S; NADH-ubiquinone oxidoreductase-F iron-sulfur binding region (PF10589; HMM-score: 12.5)
  HTH (CL0123) DUF1836; Domain of unknown function (DUF1836) (PF08876; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9192
  • Cytoplasmic Membrane Score: 0.0216
  • Cell wall & surface Score: 0.0018
  • Extracellular Score: 0.0574
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003326
  • TAT(Tat/SPI): 0.000119
  • LIPO(Sec/SPII): 0.000519
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_053039638 NCBI
• UniProt:

⊟Protein sequence

• MKSKLGITLRKVRKGKQISLYSVADEHLSKSQISRFERGESEISCIRLINILDKLHITLDEFLVLHDEDYTNTELFANLVQYIRKQYSSQNIKNIAALLSDSSHCTLNSFEKTMVKSILHTMDSTIFPSDAELLQLTDYLFKVEKWGYYEITLLGNCVRTINYNSYFLLTKEMLNNYIYSSLNKTNKRIVTQLAINCLILSVDKEEFSNCTFLITEIKALLDNELNFYEQTVFLYTTGYFEFKCHSDSGIEKMKQAIQVLDILGEDKLKLHYTSHFDKLVNKE

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0939 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Roger Junges, Gabriela Salvadori, Sudhanshu Shekhar, Heidi A Åmdal, Jimstan N Periselneris, Tsute Chen, Jeremy S Brown, Fernanda C Petersen
A Quorum-Sensing System That Regulates Streptococcus pneumoniae Biofilm Formation and Surface Polysaccharide Production.
mSphere: 2017, 2(5);
[PubMed:28932816] [WorldCat.org] [DOI] (P e)