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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 13-OCT-2024

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NCTC7465
  • locus tag: AT689_RS05805 [old locus tag: ERS445053_01136 ]
  • pan locus tag?: PNEUPAN003087000
  • symbol: AT689_RS05805
  • pan gene symbol?: ddl
  • synonym:
  • product: D-alanine--D-alanine ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: AT689_RS05805 [old locus tag: ERS445053_01136 ]
  • symbol: AT689_RS05805
  • product: D-alanine--D-alanine ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1084691..1085734
  • length: 1044
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NZ_LN831051 (1084691..1085734) NCBI
  • BioCyc: AT689_RS05805 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1484 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAACAAACGATTATTCTTTTATATGGTGGACGGAGTGCGGAACGCGAAGTCTCTGTC
    CTTTCAGCTGAGAGTGTCATGCGTGCGGTCGATTACGACCGTTTCACAGTCAAGACTTTC
    TTTATCAGTCAGTCAGGTGACTTTATCAAAACACAGGAATTTAGTCATGATCCGGGGCAA
    GAAGACCGTCTCATGACCAATGAAACCATTGATTGGGATAAGAAAGTTGCACCAAGTGCT
    ATCTACGAAGAAGGTGCAGTGGTCTTTCCAGTCCTTCACGGGCCAATGGGAGAAGATGGC
    TCTGTTCAAGGATTCTTGGAAGTTTTGAAAATGCCTTACGTTGGTTGCAACATTTTGTCA
    TCAAGTCTTGCCATGGATAAAATCACGACTAAGCGTGTTCTGGAATCTGCTGGTATTGCC
    CAAGTTCCTTATGTGGCTATCGTTGAAGGCGATGATGTGACTGCTAAAATCGCTGAAGTG
    GAAGAAAAATTGGCTTATCCAGTCTTCACTAAGCCGTCAAACATGGGGTCTAGTGTCGGT
    ATTTCTAAGTCTGAAAACCAAGAAGAACTCCGTCAAGCCTTAGAACTTGCCTTCCGATAT
    GATAGCCGTGTCTTGGTTGAGCAAGGAGTGAATGCCCGTGAAATTGAGGTTGGCCTCTTG
    GGGAACTACGATGTCAAGAGCACGCTACCAGGAGAAGTTGTCAAGGATGTTGCCTTTTAT
    GACTACGATGCCAAGTATATTGATAACAAGATTACTATGGATATTCCTGCCAAAATCAGT
    GATGATGTGGTGGCTGTCATGCGTCAAAATGCAGAAACAGCCTTCCGTGCCATTGGTGGC
    CTTGGTCTATCTCGTTGCGATTTCTTCTATACAGATAAGGGAGAGATTTTTCTCAACGAG
    CTCAATACCATGCCAGGTTTCACCCAGTGGTCTATGTACCCACTACTTTGGGACAATATG
    GGGATCAGCTACCCAGAACTAATCGAGCGTTTGGTTGACCTTGCCAAGGAAAGTTTTGAC
    AAGCGCGAAGCGCATTTGATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1044

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: AT689_RS05805 [old locus tag: ERS445053_01136 ]
  • symbol: AT689_RS05805
  • description: D-alanine--D-alanine ligase
  • length: 347
  • theoretical pI: 4.36743
  • theoretical MW: 38762.8
  • GRAVY: -0.143228

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.2.4?  ExPASy
    D-alanine--D-alanine ligase ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 330.9)
    and 11 more
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 35.1)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 29.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 26.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 25.9)
    Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 25.4)
    GNAT-family acetyltransferase TIGR03103 (TIGR03103; HMM-score: 19.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 18.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs glutamate--cysteine ligase/glutathione synthase (TIGR01435; EC 6.3.2.2,6.3.2.3; HMM-score: 17.8)
    alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 17.2)
    lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 15.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other coenzyme gamma-F420-2:alpha-L-glutamate ligase (TIGR04443; EC 6.3.2.32; HMM-score: 14.2)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    ATP-grasp (CL0179) Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 242.1)
    and 5 more
    PreATP-grasp (CL0483) Dala_Dala_lig_N; D-ala D-ala ligase N-terminus (PF01820; HMM-score: 96)
    ATP-grasp (CL0179) ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 33.8)
    CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 26.9)
    ATP-grasp_4; ATP-grasp domain (PF13535; HMM-score: 16)
    ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9857
    • Cytoplasmic Membrane Score: 0.0021
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.012
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012441
    • TAT(Tat/SPI): 0.000567
    • LIPO(Sec/SPII): 0.001803
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000814633 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQSGDFIKTQEFSHDPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEVEEKLAYPVFTKPSNMGSSVGISKSENQEELRQALELAFRYDSRVLVEQGVNAREIEVGLLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDLAKESFDKREAHLI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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