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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 13-OCT-2024

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NCTC7465
  • locus tag: AT689_RS05810 [old locus tag: ERS445053_01137 ]
  • pan locus tag?: PNEUPAN003086000
  • symbol: murF
  • pan gene symbol?: murF
  • synonym:
  • product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: AT689_RS05810 [old locus tag: ERS445053_01137 ]
  • symbol: murF
  • product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1085818..1087191
  • length: 1374
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NZ_LN831051 (1085818..1087191) NCBI
  • BioCyc: AT689_RS05810 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1483 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAATTAACAATCCATGAAATTGCCCAAGTTGTTGGAGTCAAAAATGATATCAGTATC
    TTTGAGGACACCCAGTTAGAAAAAGCTGAGTTTGATAGTCGTTTGATTGGAACTGGAGAT
    TTATTTGTGCCACTTAAAGGTGCGCGTGATGGCCATGACTTTATTGAAACAGCCTTTGAA
    AATGGTGCAGCAGTAACCTTGTCTGAGAAAGAGGTCTCAAATCATCCTTACATTCTAGTA
    GATGATGTTTTGACAGCCTTTCAATCCCTAGCATCCTACTATCTTGAAAAAACGACTGTT
    GATGTCTTTGCTGTTACAGGTTCAAATGGCAAGACAACGACTAAGGATATGTTGGCGCAT
    TTACTGTCAACAAGATACAAGACCTACAAAACACAAGGAAATTACAATAATGAGATTGGC
    CTTCCTTACACAGTTCTCCACATGCCTGAAGGAACAGAAAAGTTGGTCTTGGAGATGGGG
    CAGGACCACTTGGGAGATATTCATCTCTTGTCTGAATTGGCTCGTCCAAAAACAGCCATC
    GTGACCTTGGTTGGAGAAGCTCATTTGGCCTTTTTCAAAGATCGTTCTGAGATTGCTAAA
    GGAAAAATGCAAATTGCAGACGGAATGGCTTCAGGTTCCTTGCTTTTAGCGCCGGCTGAC
    CCTATCGTAGAGGACTATTTGCCAACTGATAAAAAGGTGGTTCGTTTTGGTCAAGGAGCA
    GAGCTGGAAATTACTGACTTGGTTGAGCGCAAAGATAGTCTAACCTTCAAGGCCAATTTC
    TTAGAGCAAGCCCTTGATTTGCCAGTAACTGGCAAGTACAATGCGACAAATGCTATGATT
    GCATCCTATGTTGCCTTGCAAGAAGGAGTTTCAGAGGAGCAAATTCGTTTGGCCTTCCAA
    GATCTTGAATTGACGCGTAACCGTACCGAATGGAAGAAAGCAGCCAATGGAGCAGATATC
    CTGTCTGATGTATACAATGCCAATCCAACTGCTATGAAGTTGATTTTAGAGACTTTCTCT
    GCCATTCCAGCCAATGAAGGTGGCAAGAAAATTGCGGTTTTAGCGGACATGAAGGAACTG
    GGTAACCAGTCAGTTCAACTCCATAATCAGATGATTTTGAGCCTTTCTCCAGATGTGCTT
    GATACCGTTATTTTCTATGGAGAAGATATTGCTGAATTAGCCCAATTGGCTAGTCAAATG
    TTCCCAATAGGCCACGTTTACTACTTCAAGAAAACAGAAGACCAAGACCAATTTGAAGAC
    CTAGTCAAGCAGGTCAAAGAAAGCCTTGGAGCCTATGACCAAATCTTGCTAAAAGGTTCT
    AACTCTATGAATCTAGCCAAATTGGTAGAACGTTTAGAAAATGAAGTCAAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1374

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: AT689_RS05810 [old locus tag: ERS445053_01137 ]
  • symbol: MurF
  • description: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
  • length: 457
  • theoretical pI: 4.60046
  • theoretical MW: 50563.2
  • GRAVY: -0.171335

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.2.10?  ExPASy
    UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (TIGR01143; EC 6.3.2.10; HMM-score: 357.2)
    and 6 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramoylalanine--D-glutamate ligase (TIGR01087; EC 6.3.2.9; HMM-score: 82.2)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramyl-tripeptide synthetase (TIGR01085; HMM-score: 78.6)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate--L-alanine ligase (TIGR01082; EC 6.3.2.8; HMM-score: 65.6)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 32.8)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (TIGR01081; HMM-score: 27.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid bifunctional protein FolC (TIGR01499; EC 6.3.2.-; HMM-score: 18.7)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    P-loop_NTPase (CL0023) Mur_ligase_M; Mur ligase middle domain (PF08245; HMM-score: 133.8)
    and 3 more
    no clan defined Mur_ligase_C; Mur ligase family, glutamate ligase domain (PF02875; HMM-score: 39.6)
    NADP_Rossmann (CL0063) Mur_ligase; Mur ligase family, catalytic domain (PF01225; HMM-score: 22.7)
    GT-B (CL0113) Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9991
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.0003
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003442
    • TAT(Tat/SPI): 0.000167
    • LIPO(Sec/SPII): 0.000231
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000778712 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKLTIHEIAQVVGVKNDISIFEDTQLEKAEFDSRLIGTGDLFVPLKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDVFAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMGQDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAPADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNATNAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKLILETFSAIPANEGGKKIAVLADMKELGNQSVQLHNQMILSLSPDVLDTVIFYGEDIAELAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVERLENEVK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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