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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 14-DEC-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NCTC7466
  • locus tag: DQM66_RS00315 [old locus tag: NCTC7466_00062 ]
  • pan locus tag?: PNEUPAN000703000
  • symbol: purB
  • pan gene symbol?: purB
  • synonym:
  • product: adenylosuccinate lyase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: DQM66_RS00315 [old locus tag: NCTC7466_00062 ]
  • symbol: purB
  • product: adenylosuccinate lyase
  • replicon: chromosome
  • strand: +
  • coordinates: 59175..60473
  • length: 1299
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_LS483374 (59175..60473) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0062 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATCAACCGTTACTCTCGCCCTGAGATGGCGAATATTTGGAGTGAAGAAAATAAATAC
    CGTGCTTGGCTTGAGGTGGAAATCCTCTCTGACGAGGCATGGGCTGAGTTGGGGGAAATC
    CCTAAGGAAGATGTGGCTTTGATTCGCAAGAAGGCGGACTTTGACATCGACCGTATTTTG
    GAAATTGAGCAGGAGACGCGCCACGATGTGGTGGCTTTCACGCGTGCGGTTTCTGAGACT
    CTTGGTGAAGAGCGCAAGTGGGTTCACTATGGGTTAACTTCTACCGACGTGGTGGATACT
    GCTTATGGTTACCTCTACAAGCAGGCCAACGACATCATCCGTCGTGACCTTGAAAACTTC
    ACTAATATCATCGCTGACAAGGCCAAGGAGCACAAGTTCACCATCATGATGGGGCGTACT
    CATGGTGTGCACGCTGAGCCGACAACTTTTGGTCTTAAATTGGCAACTTGGTATAGCGAA
    ATGAAACGCAATATCGAGCGTTTCGAGCATGCGGCTGCTGGTGTAGAAGCTGGTAAGATT
    TCTGGTGCGGTTGGGAACTTTGCCAATATCCCACCATTTGTAGAGGAGTATGTCTGCAAC
    AAGCTTGGTATCCGTGCTCAAGAAATCTCTACACAAGTCCTTCCTCGTGACCTTCACGCT
    GAGTACTTTGCAGTTCTTGCCAGCATTGCGACTTCAATCGAACGTATGGCGACTGAGATT
    CGTGGTCTACAAAAATCTGAGCAACGCGAAGTAGAAGAGTTCTTTGCTAAAGGGCAAAAA
    GGGTCTTCAGCAATGCCTCACAAACGCAACCCAATCGGTTCTGAAAATATGACTGGTCTG
    GCGCGTGTCATTCGTGGTCACATGATTACGGCTTATGAAAACGTCGCTCTCTGGCACGAA
    CGCGATATTTCCCACTCATCAGCTGAGCGTATCATCACACCAGATACGACCATTTTGATT
    GACTACATGCTCAACCGTTTTGGAAATATCGTCAAGAACTTGACAGTCTTCCCAGAAAAT
    ATGATCCGAAACATGAACTCGACTTTTGGTCTTATCTTTAGCCAACGGGCTATGTTGACA
    TTGATTGAAAAAGGCATGACCCGTGAGCAAGCCTATGACTTGGTGCAACCAAAAACAGCC
    TACTCTTGGGACAACCAAGTAGACTTTAAACCACTTCTTGAGGCAGATTCAGAAGTAACA
    TCACGTCTCACTCAAGAAGAAATCGATGAAATCTTCAACCCAGTTTATTACACCAAACGA
    GTGGATGATATCTTTGAACGTCTTGGACTAGGTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1299

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: DQM66_RS00315 [old locus tag: NCTC7466_00062 ]
  • symbol: PurB
  • description: adenylosuccinate lyase
  • length: 432
  • theoretical pI: 5.10515
  • theoretical MW: 49589.9
  • GRAVY: -0.410185

⊟Function[edit | edit source]

  • reaction:
    EC 4.3.2.2?  ExPASy
    Adenylosuccinate lyase N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis adenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 561.8)
    and 4 more
    Metabolism Energy metabolism Other 3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 164.1)
    Metabolism Amino acid biosynthesis Glutamate family argininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 74.4)
    Metabolism Energy metabolism TCA cycle fumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 50.5)
    Metabolism Energy metabolism Amino acids and amines aspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 33.6)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    no clan defined Lyase_1; Lyase (PF00206; HMM-score: 237.8)
    and 2 more
    ADSL_C; Adenylosuccinate lyase C-terminus (PF10397; HMM-score: 79)
    B2; RNA binding protein B2 (PF11473; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002165
    • TAT(Tat/SPI): 0.000121
    • LIPO(Sec/SPII): 0.000244
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000610749 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKEDVALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAYGYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSEMKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCNKLGIRAQEISTQVLPRDLHAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNLTVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPLLEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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