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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 14-DEC-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NCTC7466
  • locus tag: DQM66_RS02405 [old locus tag: NCTC7466_00459 ]
  • pan locus tag?: PNEUPAN001387000
  • symbol: glnA
  • pan gene symbol?: glnA
  • synonym:
  • product: type I glutamate--ammonia ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: DQM66_RS02405 [old locus tag: NCTC7466_00459 ]
  • symbol: glnA
  • product: type I glutamate--ammonia ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 456498..457844
  • length: 1347
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_LS483374 (456498..457844) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0448 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCAATCACAGCTGCAGATATTCGTCGTGAAGTCAAGGAAAAAAATGTTACCTTTATT
    CGTCTTATGTTCTCAGATATTTTGGGAACCATGAAAAACGTCGAAATTCCTGCTACAGAT
    GAACAGTTAGATAAGGTCTTGTCGAACAAGGTTATGTTTGATGGATCTTCTATTGAAGGT
    TTTGTACGTATCAATGAGTCGGATATGTACTTGTACCCGGACTTGGATACATGGACAGTC
    TTCCCTTGGGGAGATGAAAATGGAAGTGTTGCAGGTCTGATCTGTGATGTCTATACAACA
    GAAGGTGAACCATTTGCGGGTGACCCTCGTGGTAATTTGAAACGAGCTCTTCGTCACATG
    GAAGAAGTTGGATTCAAATCCTTCAACCTTGGTCCAGAGCCAGAATTCTTCCTATTTAAG
    TTGGATGAAAATGGGGACCCAACACTTGAAGTGAATGACAAGGGTGGCTACTTTGATTTG
    GCACCTACTGACCTTGCGGACAACACACGTCGTGAGATTGTGAATGTCTTGACCAAAATG
    GGATTTGAAGTAGAAGCGAGTCACCACGAGGTTGCGGTTGGACAGCATGAGATTGACTTT
    AAGTACGATGAAGTTCTCCGTGCTTGTGATAAGATTCAAATCTTTAAGCTTGTTGTTAAA
    ACCATTGCTCGCAAACACGGACTTTACGCAACATTTATGGCGAAGCCAAAATTTGGTATT
    GCTGGATCAGGTATGCACTGTAATATGTCCTTGTTTGATGCAGAAGGAAATAACGCCTTC
    TTTGATCCAAATGATCCAAAAGGAATGCAGTTGTCAGAAACAGCTTACCATTTCCTAGGC
    GGTTTGATCAAGCATGCTTACAACTATACTGCCATCATGAACCCAACAGTTAACTCATAC
    AAACGTTTGGTTCCAGGTTATGAAGCGCCTGTTTACATTGCTTGGGCTGGTCGTAACCGT
    TCGCCACTTGTGCGCGTACCTGCTTCACGTGGTATGGGAACTCGTCTTGAGTTGCGTTCA
    GTGGATCCAATGGCGAACCCTTACGTTGCTATGGCTGTTCTTTTGGAAGTTGGTTTGTAT
    GGTATTGAAAATAAAATCGAAGCACCAGCTCCTATCGAAGAAAATATCTACATCATGACA
    GCAGAAGAGCGCAAGGAAGCTGGTATTACAGACCTTCCATCAACTCTTCACAACGCTTTG
    AAAGCTTTGACAGAAGATGAAGTGGTTAAAGCTGCTCTCGGAGATCACATCTACACTAGC
    TTCCTTGAAGCCAAACGAATCGAATGGGCAAGTTATGCAACCTTCGTTTCACAATGGGAA
    ATTGATAATTATTTAGACCTTTACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1347

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: DQM66_RS02405 [old locus tag: NCTC7466_00459 ]
  • symbol: GlnA
  • description: type I glutamate--ammonia ligase
  • length: 448
  • theoretical pI: 4.67862
  • theoretical MW: 50321.9
  • GRAVY: -0.232143

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.1.2?  ExPASy
    Glutamine synthetase ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family glutamine synthetase, type I (TIGR00653; EC 6.3.1.2; HMM-score: 558.2)
    and 1 more
    glutamine synthetase, type III (TIGR03105; EC 6.3.1.2; HMM-score: 361.5)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    GCS (CL0286) Gln-synt_C; Glutamine synthetase, catalytic domain (PF00120; HMM-score: 454.3)
    and 1 more
    no clan defined Gln-synt_N; Glutamine synthetase, beta-Grasp domain (PF03951; HMM-score: 98.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003788
    • TAT(Tat/SPI): 0.001575
    • LIPO(Sec/SPII): 0.000782
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001122904 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLDKVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEGEPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDLAPTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLVVKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAYHFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGTRLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDLPSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Tomas G Kloosterman, Wouter T Hendriksen, Jetta J E Bijlsma, Hester J Bootsma, Sacha A F T van Hijum, Jan Kok, Peter W M Hermans, Oscar P Kuipers
Regulation of glutamine and glutamate metabolism by GlnR and GlnA in Streptococcus pneumoniae.
J Biol Chem: 2006, 281(35);25097-109
[PubMed:16787930] [WorldCat.org] [DOI] (P p)

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