PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 12-FEB-2025

⊟Summary

• organism: Streptococcus pneumoniae NCTC7466
• locus tag: DQM66_RS02575 [old locus tag: NCTC7466_00492 ]
• pan locus tag^?: PNEUPAN001429000
• symbol: trmB
• pan gene symbol^?: trmB
• synonym:
• product: tRNA (guanosine(46)-N7)-methyltransferase TrmB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: DQM66_RS02575 [old locus tag: NCTC7466_00492 ]
• symbol: trmB
• product: tRNA (guanosine(46)-N7)-methyltransferase TrmB
• replicon: chromosome
• strand: +
• coordinates: 486856..487491
• length: 636
• essential: unknown

⊟Accession numbers

• Location: NZ_LS483374 (486856..487491) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0477 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGAGAGTTAGAAATCGTAAAGGGGCAACAGAATTACTAGAGGCAAATCCCCAGTATGTG
  GTCCTCAATCCCTTGGAAGCCAAGGCAAAATGGCGGGACTTGTTTGGCAATGATAATCCC
  ATTCATGTGGAAGTTGGAAGTGGAAAGGGTGCCTTTGTTTCAGGTATGGCCAAGCAAAAC
  CCTGACATCAACTATATCGGGATTGATATTCAAAAATCTGTTTTGAGCTACGCTTTGGAC
  AAGGTGCTTGAAGTTGGAGTGCCTAACATCAAGCTCTTGTGGGTAGATGGTTCTGACTTG
  ACTGACTACTTTGAAGACGGTGAGATTGATCGCTTGTATCTGAACTTTTCAGATCCATGG
  CCGAAAAAACGCCATGAAAAGCGTCGTTTGACCTACAAGACCTTTTTGGACACCTTCAAA
  CGTATCTTGCCTGAAAATGGAGAAATTCATTTCAAGACGGATAACCGTGGCTTGTTTGAG
  TACAGTTTAGTGAGCTTTTCTCAATATGGCATGAAACTCAATGGTGTCTGGCTAGATTTG
  CATGCCAGTGATTTTGAAGGCAATGTCATGACAGAATACGAGCAAAAATTCTCAAACAAG
  GGGCAAGTTATCTACCGAGTTGAGGCAGAATTTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  636
  

⊟Protein

⊟General

• locus tag: DQM66_RS02575 [old locus tag: NCTC7466_00492 ]
• symbol: TrmB
• description: tRNA (guanosine(46)-N7)-methyltransferase TrmB
• length: 211
• theoretical pI: 6.14512
• theoretical MW: 24378.5
• GRAVY: -0.508057

⊟Function

• reaction:
  EC 2.1.1.33?  ExPASy
  tRNA (guanine⁴⁶-N⁷)-methyltransferase S-adenosyl-L-methionine + guanine⁴⁶ in tRNA = S-adenosyl-L-homocysteine + N⁷-methylguanine⁴⁶ in tRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 192.2)
  and 5 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 15.4)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 15)
  2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 12.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 11.5)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 10.7)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• PFAM:
  NADP_Rossmann (CL0063) Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 159.8)
  and 8 more
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 28.9)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 27.2)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 25.6)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 21.4)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 17.6)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 12.9)
  UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 12.1)
  Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9926
  • Cytoplasmic Membrane Score: 0.0006
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0067
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.013705
  • TAT(Tat/SPI): 0.001059
  • LIPO(Sec/SPII): 0.0007
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_001266083 NCBI
• UniProt:

⊟Protein sequence

• MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVEVGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTDYFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFEYSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0477 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications