From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-NOV-2024

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae EF3030
  • locus tag: EF3030_RS02705 [old locus tag: EF3030_02705 ]
  • pan locus tag?: PNEUPAN001455000
  • symbol: EF3030_RS02705
  • pan gene symbol?: —
  • synonym:
  • product: ATP-dependent nuclease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EF3030_RS02705 [old locus tag: EF3030_02705 ]
  • symbol: EF3030_RS02705
  • product: ATP-dependent nuclease
  • replicon: chromosome
  • strand: +
  • coordinates: 496561..498039
  • length: 1479
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_CP035897 (496561..498039) NCBI
  • BioCyc: EF3030_RS02705 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    GTGTCTGAATATTGGTTTAGTACAAATGTTGATCAAATAGATGAAGTGGATGGGAAACAA
    TGTTTAATCTATTCATATTATAACGTTAAAGCTTCTAGAAATGTAGAAGTTCTTAAAGGC
    CGTTCTGGAACAAAAAAAGGATTAGATTATTGGGAACCATATGCTCCTCAAAAACAATAT
    GAGATGGAAAGATTACCCAAAAATAAATATATTGGAAGTTCTTCGACAGACAGATGGGAT
    GGCATTGAAAAAAATGTTGTCTTTTGTGATTGTAAAGAATATGTTTCTGCTTTTGATTTA
    TTTTTCTATCACTATAATTTTAAAAAAATTTCTACTCAACGTAGTAAACAAGATTTTATA
    AGATTACGATCTAAACCAGTTGCTGATATTTTAAAGAATAATACAAGTAGTTATACGCGT
    TATAAAAAGGAAATGGTTATAGATAATGTTAAAGTTGATGATAAAGTTTGTGAAATTATT
    AGTGAAATAATGGATGAATCTTATACAGACATTCAAATTTTAACACATAAGTTGTATTCA
    AAAGGTGATGATATAAAAGCTTCCAAAACTATTTGGATGAAAAAATCTGGAAAAGAATAT
    TCCGAGGCTTTTGCAGGAACAGGTGAGGCTAGAATTATTTTGTTAGTAAATGATATAGTA
    AATGCGCAATCAAATTCATTAATATTAATAGATGAACCTGAAATCTCACTTCATCCGAGT
    GCAATCTATAAATTTAAAGAGTTTTTACTTCAAGAGTGTTTAAATAAAAAACATCAAATT
    ATTATCACTACACATTCTACACAACTTATAAAAGATTTTCTTAGAGAAGCCGTGAAACTT
    TTAGTGAAAAACGGAGAAAAGGTAGATGTTATTGAAAATATTGATTATCAGGATGCATTT
    TTTGAATTAGGTGATGTGTATCATTCTAGGAAGATGATTTATGTTGAAGATAGACTAGCT
    AAATATATTCTAGAGTTTGTTATCACTCATTCAGGTAGTGAGAATCTTAAACAGAATTTA
    GTAGTGAGATATATTCCTGGTGGAGCAAATCAAATAATTTGTAATAATATTTTAAACTCA
    TCGTATTTAGATTCCGATAACCATTATTTTTGGCTTGATGGAGATCAAAACACTAATGTT
    AGTGAATCAAATAATTTAATGAACTATCTTGAAAATGGTGTTGTTATATCAGATAAAATT
    CCTGAATCAGATAATAAAAATCTTGATGATATTATAAAATTGATAACGGGATGTCCAATT
    AAATTTAATGTTTCAGGTAATAAAGGGCAAAAAAATAATATTGAATTAATTGCGAAACAA
    AGAAGCTTTATAGATTATTGGGCTAAATACGTTTCTTATCTTCCTTTCCCAACCCCAGAG
    TTTTTTTTAGCTAATTTGTGTAACAGCGTAGATAGGGAAGGTTATGATTTTTCTAAAGAT
    GGCAACGGAAAAGAATATTTTAGAAAAAAACTCAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1479

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EF3030_RS02705 [old locus tag: EF3030_02705 ]
  • symbol: EF3030_RS02705
  • description: ATP-dependent nuclease
  • length: 492
  • theoretical pI: 6.5239
  • theoretical MW: 57076.4
  • GRAVY: -0.506911

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    restriction system-associated AAA family ATPase (TIGR04435; HMM-score: 26)
    Metabolism Energy metabolism Methanogenesis methyl coenzyme M reductase system, component A2 (TIGR03269; HMM-score: 21.3)
    and 10 more
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 19.8)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 17)
    Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 15.5)
    Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 15.5)
    Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 12.7)
    Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 12.7)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking lipoprotein releasing system, ATP-binding protein (TIGR02211; EC 3.6.3.-; HMM-score: 12.3)
    Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 12.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 11.2)
    Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 11.2)
  • TheSEED: data available for Hungary19A-6, TIGR4
  • PFAM:
    P-loop_NTPase (CL0023) AAA_15; AAA ATPase domain (PF13175; HMM-score: 61.3)
    AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 49.9)
    and 1 more
    DUF2813; Protein of unknown function (DUF2813) (PF11398; HMM-score: 17.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0.1646
    • Cytoplasmic Membrane Score: 0.0919
    • Cell wall & surface Score: 0.0005
    • Extracellular Score: 0.743
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.028963
    • TAT(Tat/SPI): 0.000782
    • LIPO(Sec/SPII): 0.002174
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001828244 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSEYWFSTNVDQIDEVDGKQCLIYSYYNVKASRNVEVLKGRSGTKKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFFYHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEIISEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVNDIVNAQSNSLILIDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFLREAVKLLVKNGEKVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSENLKQNLVVRYIPGGANQIICNNILNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLENGVVISDKIPESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKYVSYLPFPTPEFFLANLCNSVDREGYDFSKDGNGKEYFRKKLK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=EF3030_RS02705&oldid=13864"