PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 12-FEB-2025

⊟Summary

• organism: Streptococcus pneumoniae ATCC 49619
• locus tag: EL263_RS03540 [old locus tag: SPAT_0660 ]
• pan locus tag^?: PNEUPAN001647000
• symbol: thiE
• pan gene symbol^?: thiE-2
• synonym:
• product: thiamine phosphate synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: EL263_RS03540 [old locus tag: SPAT_0660 ]
• symbol: thiE
• product: thiamine phosphate synthase
• replicon: chromosome
• strand: +
• coordinates: 667153..667785
• length: 633
• essential: unknown

⊟Accession numbers

• Location: NZ_AP018938 (667153..667785) NCBI
• BioCyc: EL263_RS03540 BioCyc
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0631 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  301
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  481
  541
  601
  
  ATGAATAGAGAAGCACTTAGACTATATCTGGTAACCAATCGCTATCAAGATTCCGTGGAA
  AGCTTTCTTGCAAAAGTTGAGACGGCCTGCCGTTCAGGGGTTACCATAGTCCAATTGCGA
  GAAAAAAATCTCACAACCAATCAATATTATCAACTGGCAAAACAAGTCAAGGAAATAACA
  GATGCTTATCAGGTACCCTTGATAATCGATGATCGGTTGGATGTTTGTCTTGCGGTTGAT
  GCTGCTGGTCTGCATATTGGAGATGACGAACTCCCAGTTTCGGTTGCCCGACAAGTCTTG
  GGTCCTGAAAAAATCCTCGGTGTCACCGCTAAAACGGTTAAAAGAGCTCTGGAAGCGGAG
  AAATCAGGTGCAGATTACTTGGGGACAGGAGCCATTTTTCCGACAACCACCAAGGAAAAT
  GCACCCATCACCCTGATTTCAACCTTGAAAACAATTTGCCAAACGGTTGCCATTCCAATA
  GTTGCTATTGGCGGCTTGACATCAGAGAATATTGACCAACTTATCGGCACGGGTATAGCT
  GGTGTAGCTGTTGTACGTGATTTGATGCAAGCAGAAGATATTGAGGCAAAAACGCAAGCC
  TTTTTAACAAAGTTGCATGACATTCTTTCCTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  633
  

⊟Protein

⊟General

• locus tag: EL263_RS03540 [old locus tag: SPAT_0660 ]
• symbol: ThiE
• description: thiamine phosphate synthase
• length: 210
• theoretical pI: 4.8933
• theoretical MW: 22740.1
• GRAVY: 0.114762

⊟Function

• reaction:
  EC 2.5.1.3?  ExPASy
  Thiamine phosphate synthase 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO₂ 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO₂ 4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 223.8)
  and 5 more
  3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 16.9)
  Metabolism Amino acid biosynthesis Histidine family imidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 16.3)
  predicted phospho-2-dehydro-3-deoxyheptonate aldolase (TIGR01949; EC 4.2.1.-; HMM-score: 16.2)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis fructose-1,6-bisphosphate aldolase, class II (TIGR01859; EC 4.1.2.13; HMM-score: 13.7)
  glycosyl amidation-associated protein WbuZ (TIGR03572; HMM-score: 12.9)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• PFAM:
  TIM_barrel (CL0036) TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 211.8)
  and 6 more
  NMO; Nitronate monooxygenase (PF03060; HMM-score: 25.3)
  PK_TIM (CL0151) PEP_mutase; Phosphoenolpyruvate phosphomutase (PF13714; HMM-score: 16.5)
  PELOTA (CL0101) RNase_P_pop3; RNase P subunit Pop3 (PF08228; HMM-score: 15.4)
  TIM_barrel (CL0036) DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 14)
  no clan defined BORCS6; BLOC-1-related complex sub-unit 6 C-terminal helix (PF10157; HMM-score: 12.2)
  EMP70; Endomembrane protein 70 (PF02990; HMM-score: 11)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9981
  • Cytoplasmic Membrane Score: 0.0002
  • Cell wall & surface Score: 0.0004
  • Extracellular Score: 0.0013
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007219
  • TAT(Tat/SPI): 0.001219
  • LIPO(Sec/SPII): 0.008874
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_001078229 NCBI
• UniProt:

⊟Protein sequence

• MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGPEKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPIVAIGGLTSENIDQLIGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0631 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications