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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae ATCC 49619
  • locus tag: EL263_RS05265 [old locus tag: SPAT_0987 ]
  • pan locus tag?: PNEUPAN000112000
  • symbol: metG
  • pan gene symbol?: metG
  • synonym:
  • product: methionine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EL263_RS05265 [old locus tag: SPAT_0987 ]
  • symbol: metG
  • product: methionine--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 978098..979735
  • length: 1638
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_AP018938 (978098..979735) NCBI
  • BioCyc: EL263_RS05265 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCAATTTTTATTGGAGGAGCATGGCCATATGCAAACGGTTCGTTACATATTGGTCAC
    GCGGCAGCGCTTTTACCGGGGGATATTCTTGCAAGATACTATCGTCAGAAGGGAGAGGAA
    GTTTTATATGTTTCTGGAAGTGATTGTAATGGAACCCCTATTTCTATCAGAGCTAAAAAA
    GAAAATAAGTCTGTGAAAGAAATTGCTGATTTTTATCATAAGGAATTTAAAGAAACTTTT
    GAAAAATTAGGTTTTACATATGACTTGTACTCGCGAACAGATTCTCCACTACATCATGAG
    ATAGTACAAGAATTATTTTTGCAATTATACGAGAAGAAATTTTTGTATACAAAAAAAATT
    AAACAACTCTATTGTACATTTGATAATCAATTCTTACCTGATAGGTTCGTTGAAGGAAAA
    TGCCCGAATTGTGGAACCCATTCAAGAGGAGATCAATGTGATAATTGCTCAGCAATTTTA
    GATCCTATAGATTTAGTTGATAAAAGATGTTCTATTTGTTCCAATGAACCAGAAGTTCGT
    GAGACAGAACACTTTTATTATGTTTTTAGTGAGTTTCAAAATTTGTTAGAGACTTACTTA
    AATGATGCTGAAGAAACAGTGAGATGGCGAAAAAATGCAATAAATCTAACTAAAAGGTAT
    TTACGGGAAGGTTTACCTGATCGAGCTGTTACTCGTGATCTACCAAATGGGATTCCAGTT
    CCCATTGATGGTTTTAGAGATAAAAAAATATATGTCTGGTTTGAAGCGGTAGCAGGTTAC
    TACACAGCAAGTGTTGATTGGGCGCAAAAACTTCAAAATAATATTACTGATTTTTGGAAC
    AATCGGACAAAGTCATATTATGTTCATGGAAAAGATAACATCCCATTTCATACTATTATT
    TGGCCGGCAATACTCTCAGGTTTAGAAATAGAGCCTCTTCCAGAGTATATTATTTCTAGT
    GAATATTTAACGTTAGAAAACAAAAAAATATCAACAAGTAATAATTGGGCTATATGGCTT
    AACGATATCATAAAAAAATATGATGCTGATTCAATCCGATACTTCCTAACAATAAATGCT
    CCTGAGATGAAAGATGCAAATTTTTCTTGGAGAGAATTTATTTATAGTCATAACTCAGAA
    TTATTAGGATCGTACGGTAATTTTATCAATAGGACTCTAAAGTTTATTGAAAAGTATTTT
    GAATCTGAAATACCCACTAAATATCTCGAAGGAGAAATTTTATATAATTTGAAAGAATTA
    TATACAACAGTAGGTAATTTAGTTGAGAGCGGGCATATGAAACAGGCATTAGAAGAAATT
    TTTGAATATATTAGATCGGCTAATAAATTCTATGATGATATGAAACCGTGGGCATTGCGA
    GAGTCCGATATTGAAAAATGTAAAGAAGTCTTAGCTACTTGTGTAATCATTATACTTAAT
    TTAGGACAAATGTTAAATCCATTCATTCCTTTTTCTGGTAAAAAGATTGAGGATATGTTT
    AAAACAAAGCTAAATACTTGGAATTATATTTCAAACCTTCCTAACAAGCTATCAGATGTA
    TCAATGTTGTTTGATAGGATTGATTTGAAAAAAATTGACGAGGAAGTTTTAGAATTGCAA
    CAAACATCCAGTAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1638

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EL263_RS05265 [old locus tag: SPAT_0987 ]
  • symbol: MetG
  • description: methionine--tRNA ligase
  • length: 545
  • theoretical pI: 5.5724
  • theoretical MW: 63688
  • GRAVY: -0.428807

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.10?  ExPASy
    Methionine--tRNA ligase ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 514.3)
    and 7 more
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 89.6)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 89.4)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 82.8)
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 73.1)
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 49.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 20.5)
    Genetic information processing Protein synthesis tRNA aminoacylation arginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 8.9)
  • TheSEED:
  • PFAM:
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 454.4)
    and 11 more
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 72.5)
    tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 52)
    DALR (CL0258) Anticodon_3; Anticodon binding domain of methionyl tRNA ligase (PF19303; HMM-score: 24.9)
    Anticodon_1; Anticodon-binding domain of tRNA ligase (PF08264; HMM-score: 23.4)
    EGF (CL0001) Laminin_EGF; Laminin EGF domain (PF00053; HMM-score: 15.1)
    HUP (CL0039) tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 14.5)
    no clan defined YfmQ; Uncharacterised protein from bacillus cereus group (PF10787; HMM-score: 13.6)
    Zn_Beta_Ribbon (CL0167) DZR; Double zinc ribbon (PF12773; HMM-score: 12.5)
    no clan defined Synapsin_N; Synapsin N-terminal (PF10581; HMM-score: 11.7)
    PolC_DP2; DNA polymerase II large subunit DP2 (PF03833; HMM-score: 11.1)
    RecR; RecR protein (PF02132; HMM-score: 7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9997
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0003
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.055245
    • TAT(Tat/SPI): 0.007317
    • LIPO(Sec/SPII): 0.011972
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000021543 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSIFIGGAWPYANGSLHIGHAAALLPGDILARYYRQKGEEVLYVSGSDCNGTPISIRAKKENKSVKEIADFYHKEFKETFEKLGFTYDLYSRTDSPLHHEIVQELFLQLYEKKFLYTKKIKQLYCTFDNQFLPDRFVEGKCPNCGTHSRGDQCDNCSAILDPIDLVDKRCSICSNEPEVRETEHFYYVFSEFQNLLETYLNDAEETVRWRKNAINLTKRYLREGLPDRAVTRDLPNGIPVPIDGFRDKKIYVWFEAVAGYYTASVDWAQKLQNNITDFWNNRTKSYYVHGKDNIPFHTIIWPAILSGLEIEPLPEYIISSEYLTLENKKISTSNNWAIWLNDIIKKYDADSIRYFLTINAPEMKDANFSWREFIYSHNSELLGSYGNFINRTLKFIEKYFESEIPTKYLEGEILYNLKELYTTVGNLVESGHMKQALEEIFEYIRSANKFYDDMKPWALRESDIEKCKEVLATCVIIILNLGQMLNPFIPFSGKKIEDMFKTKLNTWNYISNLPNKLSDVSMLFDRIDLKKIDEEVLELQQTSSR

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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