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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae HU-OH
  • locus tag: EL271_RS03695 [old locus tag: SPOH_0676 ]
  • pan locus tag?: PNEUPAN001750000
  • symbol: ciaH
  • pan gene symbol?: ciaH
  • synonym:
  • product: two-component system sensor histidine kinase CiaH

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EL271_RS03695 [old locus tag: SPOH_0676 ]
  • symbol: ciaH
  • product: two-component system sensor histidine kinase CiaH
  • replicon: chromosome
  • strand: +
  • coordinates: 684758..686092
  • length: 1335
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_AP018937 (684758..686092) NCBI
  • BioCyc: EL271_RS03695 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0702 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTTCAGTAAACTTAAAAAAACATGGTATGCGGATGACTTTAGTTATTTTATCCGCAAC
    TTCGGTGTCTTCACCCTGATTTTTTCTACAATGACTCTGATTATTTTACAAGTCATGCAT
    TCGAGTCTTTATACTTCGGTGGACGATAAGCTTCATGGACTGAGTGAAAATCCTCAAGCA
    GTTATTCAGCTGGCTATAAATAGGGCAACAGAAGAGATTAAAGATTTAGAAAATGCTAGG
    GCGGACGCTAGTAAAGTAGAAATAAAACCTAATGTCAGTTCCAATACGGAAGTCATTCTC
    TTTGATAAAGACTTTACTCAACTTCTTTCTGGAAATCGATTTTTGGGCTTGGATAAGATT
    AAGTTAGAAAAGAAAGAACTAGGACATATCTACCAGATTCAGGTTTTTAATAGCTATGGG
    CAGGAAGAAATCTATCGTGTGATTTTGATGGAGACCAATATTAGTTCGGTTTCAACCAAT
    ATCAAGTATGCTGCTGTCTTGATTAATACCAGTCAGTTGGAGCAGGCTAGTCAAAAGCAT
    GAGCAATTGATTGTGGTCGTGATGGCTAGTTTCTGGATTTTGTCTTTACTTGCCAGTCTC
    TATCTAGCTAGGGTCAGTGTTAGGCCCCTGCTTGAGAGTATGCAGAAGCAACAGTCTTTT
    GTGGAAAATGCCAGTCATGAGTTACGAACTCCACTCGCAGTTTTGCAAAATCGCTTAGAG
    ACCCTTTTTCGTAAGCCAGAAGCTACCATTATGGATGTGAGCGAAAGCATTGCATCGAGT
    TTGGAAGAAGTCCGAAATATGCGTTTTTTAACGACAAGCTTGCTGAACTTAGCTCGGAGA
    GATGATGGGATTAAGCCGGAGCTTGCAGAAGTTCCAACTAGCTTTTTTAATACAACTTTC
    ACAAACTATGAGATGATTGCTTCGGAAAATAATCGTGTCTTCCGTTTTGAAAATCGTATC
    CATCGAACAATTGTCACAGATCAGCTTCTTCTGAAACAACTGATGACCATTCTTTTCGAT
    AATGCCGTCAAGTATACTGAGGAGGATGGTGAAATTGATTTTCTTATCTCGGCGACCGAT
    CGCAATCTTTATTTACTTGTTTCTGATAATGGAATCGGTATTTCGACAGAAGATAAAAAG
    AAAATTTTTGACCGTTTTTATCGAGTAGACAAGGCTAGAACCCGGCAAAAAGGTGGTTTT
    GGTTTAGGATTATCCCTAGCCAAGCAAATTGTAGATGCTCTAAAAGGAACTGTTACTGTC
    AAAGATAATAAACCCAAGGGAACAATCTTTGAAGTGAAGATTGCCATTCAGACACCATCT
    AAAAAGAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1335

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EL271_RS03695 [old locus tag: SPOH_0676 ]
  • symbol: CiaH
  • description: two-component system sensor histidine kinase CiaH
  • length: 444
  • theoretical pI: 9.20524
  • theoretical MW: 50673
  • GRAVY: -0.166441

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 150.4)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 138.4)
    and 10 more
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 79.5)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 68.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 42.6)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 42.6)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 29.8)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 29.8)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase VI, B subunit (TIGR01052; EC 5.99.1.3; HMM-score: 15.1)
    Cellular processes Cellular processes Sporulation and germination anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 13.5)
    Signal transduction Regulatory functions Protein interactions anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 13.5)
    anti-sigma B factor (TIGR01924; EC 2.7.11.1; HMM-score: 12.7)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 84)
    and 5 more
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 36.4)
    HATPase_c_5; GHKL domain (PF14501; HMM-score: 20.6)
    HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 16.7)
    NADP_Rossmann (CL0063) GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 14.4)
    no clan defined DUF1415; Protein of unknown function (DUF1415) (PF07209; HMM-score: 11)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.9988
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0012
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.046733
    • TAT(Tat/SPI): 0.001854
    • LIPO(Sec/SPII): 0.002169
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000491790 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLYTSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFDKDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTNIKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQSFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLNLARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQLMTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKARTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Thorsten Mascher, Dorothea Zähner, Michelle Merai, Nadège Balmelle, Antoine B de Saizieu, Regine Hakenbeck
The Streptococcus pneumoniae cia regulon: CiaR target sites and transcription profile analysis.
J Bacteriol: 2003, 185(1);60-70
[PubMed:12486041] [WorldCat.org] [DOI] (P p)Adilia Dagkessamanskaia, Miriam Moscoso, Vincent Hénard, Sébastien Guiral, Karin Overweg, Mark Reuter, Bernard Martin, Jerry Wells, Jean-Pierre Claverys
Interconnection of competence, stress and CiaR regulons in Streptococcus pneumoniae: competence triggers stationary phase autolysis of ciaR mutant cells.
Mol Microbiol: 2004, 51(4);1071-86
[PubMed:14763981] [WorldCat.org] [DOI] (P p)Alexander Halfmann, Márta Kovács, Regine Hakenbeck, Reinhold Brückner
Identification of the genes directly controlled by the response regulator CiaR in Streptococcus pneumoniae: five out of 15 promoters drive expression of small non-coding RNAs.
Mol Microbiol: 2007, 66(1);110-26
[PubMed:17725562] [WorldCat.org] [DOI] (P p)Alexander Halfmann, Anke Schnorpfeil, Miriam Müller, Patrick Marx, Ulrike Günzler, Regine Hakenbeck, Reinhold Brückner
Activity of the two-component regulatory system CiaRH in Streptococcus pneumoniae R6.
J Mol Microbiol Biotechnol: 2011, 20(2);96-104
[PubMed:21422763] [WorldCat.org] [DOI] (I p)

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