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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 12-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NU83127
  • locus tag: EL277_RS02930 [old locus tag: SPNU_0552 ]
  • pan locus tag?: PNEUPAN001471000
  • symbol: pheS
  • pan gene symbol?: pheS
  • synonym:
  • product: phenylalanine--tRNA ligase subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EL277_RS02930 [old locus tag: SPNU_0552 ]
  • symbol: pheS
  • product: phenylalanine--tRNA ligase subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 552488..553534
  • length: 1047
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_AP018936 (552488..553534) NCBI
  • BioCyc: EL277_RS02930 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0504 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCAACTATTGAAGAACAATTAAAAGCGCTTCGCGAAGAAACGCTGGCTAGCTTGAAG
    CAGATTACTGCTGGAAATGAAAAAGAGATGCAAGATTTGCGTGTCTCTGTCCTTGGTAAA
    AAGGGTTCGCTCACTGAAATCCTCAAAGGGATGAAAGATGTTTCTGCTGAGATGCGTCCA
    ATCATCGGGAAACACGTCAATGAAGCTCGTGATGTCTTGACTGCTGCTTTTGAAGAAACA
    GCTAAGCTCTTGGAAGAAAAGAAAGTCGCGGCTCAACTGGCTAGCGAGAGTATAGATGTG
    ACGCTTCCAGGTCGTCCAGTTGCGACTGGTCACCGTCACGTTTTGACACAAACCAGTGAA
    GAAATCGAAGATATCTTCATCGGTATGGGTTATCAAGTCGTGGATGGTTTTGAAGTGGAG
    CAAGACTACTATAACTTTGAACGTATGAACCTTCCAAAAGACCACCCAGCTCGTGATATG
    CAGGATACTTTCTATATCACTGAAGAAATCTTGCTCCGTACCCACACGTCTCCAGTTCAG
    GCGCGTGCTATGGATGCCCATGATTTTTCTAAAGGTCCTTTGAAGATGATCTCGCCAGGG
    CGTGTCTTCCGTCGCGATACGGACGATGCGACCCACAGTCACCAATTCCACCAAATCGAA
    GGCTTGGTAGTTGGGAAAAATATCTCTATGGCTGATCTTCAAGGAACGCTTCAGTTGATT
    GTCCAAAAAATGTTTGGTGAAGAGCGTCAAATTCGTTTGCGTCCATCTTACTTCCCATTC
    ACAGAGCCATCTGTTGAGGTGGATGTTTCTTGCTTCAAGTGTGGTGGAGAAGGCTGTAAT
    GTATGTAAGAAAACAGGTTGGATCGAAATTATGGGGGCCGGTATGGTTCACCCACGTGTC
    CTTGAAATGAGTGGTATCGATGCGACTGTATACTCTGGCTTTGCCTTTGGTCTTGGACAA
    GAGCGTGTAGCTATGCTCCGTTATGGAATCAACGATATCCGTGGATTCTACCAAGGAGAT
    GTCCGCTTCTCAGAACAGTTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1047

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EL277_RS02930 [old locus tag: SPNU_0552 ]
  • symbol: PheS
  • description: phenylalanine--tRNA ligase subunit alpha
  • length: 348
  • theoretical pI: 5.46801
  • theoretical MW: 39101.4
  • GRAVY: -0.336494

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.20?  ExPASy
    Phenylalanine--tRNA ligase ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 357.7)
    and 7 more
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase (TIGR00469; EC 6.1.1.20; HMM-score: 114.6)
    Genetic information processing Protein synthesis tRNA aminoacylation O-phosphoserine--tRNA ligase (TIGR00470; EC 6.1.1.27; HMM-score: 51.7)
    Metabolism Amino acid biosynthesis Serine family O-phosphoserine--tRNA ligase (TIGR00470; EC 6.1.1.27; HMM-score: 51.7)
    Metabolism Amino acid biosynthesis Histidine family ATP phosphoribosyltransferase, regulatory subunit (TIGR00443; HMM-score: 23.2)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 18.2)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, beta subunit (TIGR00471; EC 6.1.1.20; HMM-score: 17.6)
    Genetic information processing Protein synthesis tRNA aminoacylation histidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 16.6)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 361)
    and 3 more
    tRNA_bind_arm (CL0298) Phe_tRNA-synt_N; Aminoacyl tRNA synthetase class II, N-terminal domain (PF02912; HMM-score: 70.8)
    tRNA_synt_II (CL0040) tRNA_synthFbeta; Phenylalanyl tRNA synthetase beta chain CLM domain (PF17759; HMM-score: 16.9)
    tRNA-synt_His; Histidyl-tRNA synthetase (PF13393; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9986
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0014
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004245
    • TAT(Tat/SPI): 0.002692
    • LIPO(Sec/SPII): 0.000804
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001818763 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSLTEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTLPGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDMQDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGGEGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIRGFYQGDVRFSEQFK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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