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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 14-DEC-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NU83127
  • locus tag: EL277_RS04675 [old locus tag: SPNU_0886 ]
  • pan locus tag?: PNEUPAN001933000
  • symbol: EL277_RS04675
  • pan gene symbol?: cmbR
  • synonym:
  • product: LysR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EL277_RS04675 [old locus tag: SPNU_0886 ]
  • symbol: EL277_RS04675
  • product: LysR family transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 888133..889041
  • length: 909
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_AP018936 (888133..889041) NCBI
  • BioCyc: EL277_RS04675 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0818 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAACATTCAACAATTACGCTATGTTGTGGCTATTGCCAATAGTGGTACTTTTCGTGAA
    GCTGCTGAAAAGATGTATGTTAGTCAGCCGAGTCTGTCTATTTCTGTTCGTGATTTGGAA
    AAAGAGTTGGGCTTTAAGATTTTCCGTCGGACCAGCTCAGGGACTTTCTTGACCCGTCGT
    GGGATGGAATTTTATGAAAAAGCGCAAGAATTGGTTAAAGGATTTGATATTTTTCAAAAT
    CAGTATGCCAATCCTGAAGAAGAAAAAGATGAATTTTCCGTTGCTAGCCAGCACTATGAC
    TTCTTACCACCAACTATTACGGCCTTTTCAGAGCGCTATCCTGACTATAAGAACTTCCGT
    ATTTTTGAATCAACTACTGTTCAAATATTAGATGAAGTGGCGCAAGGGCATAGTGAGATT
    GGGATTATCTACCTCAACAATCAAAATAAAAAGGGGATTATGCAACGGGTTGAAAAGTTA
    GGTCTGGAGGTCATCGAATTGATTCCTTTCCATACCCATATTTATCTCTGTGAGGGTCAT
    CCTTTAGCCCAGAAAGAGGAATTAGTCATGGAGGATTTAGCGGATTTACCAACGGTTCGT
    TTCACTCAAGAGAAAGACGAGTACCTTTATTATTCAGAGAACTTTGTTGATACCAGCGCT
    AGCTCACAGATGTTTAATGTGACAGACCGTGCCACCTTGAATGGTATTTTGGAGCGGACG
    GACGCCTATGCGACAGGTTCTGGATTTTTAGATAGTGACAGTGTTAATGGCATTACAGTT
    ATTCGTCTCAAGGATAACCTAGATAACCGCATGGTCTATGTTAAACGTGAAGAAGTGGAG
    CTTAGTCAAGCTGGGACTCTCTTCGTAGAAGTCATGCAAGAATATTTTGATCAAAAGAGG
    AAATCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    909

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EL277_RS04675 [old locus tag: SPNU_0886 ]
  • symbol: EL277_RS04675
  • description: LysR family transcriptional regulator
  • length: 302
  • theoretical pI: 4.61633
  • theoretical MW: 34907
  • GRAVY: -0.447682

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 56.9)
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 52.5)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 52.5)
    putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 48.4)
    and 4 more
    Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.6)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.6)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 29.6)
    Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 25.3)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 62.6)
    and 3 more
    PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 48.4)
    HTH (CL0123) MarR_2; MarR family (PF12802; HMM-score: 15.8)
    no clan defined Spectrin_like; Spectrin like domain (PF18373; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by CmbR*, TF important in Cysteine metabolism: in TIGR4

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008189
    • TAT(Tat/SPI): 0.001011
    • LIPO(Sec/SPII): 0.001068
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001025704 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELGFKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFLPPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKLGLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDTSASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKREEVELSQAGTLFVEVMQEYFDQKRKS

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Muhammad Afzal, Irfan Manzoor, Oscar P Kuipers, Sulman Shafeeq
Cysteine-Mediated Gene Expression and Characterization of the CmbR Regulon in Streptococcus pneumoniae.
Front Microbiol: 2016, 7;1929
[PubMed:27990139] [WorldCat.org] [DOI] (P e)

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