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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 14-DEC-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NU83127
  • locus tag: EL277_RS05920 [old locus tag: SPNU_1124 ]
  • pan locus tag?: PNEUPAN002464000
  • symbol: lepA
  • pan gene symbol?: lepA
  • synonym:
  • product: translation elongation factor 4

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EL277_RS05920 [old locus tag: SPNU_1124 ]
  • symbol: lepA
  • product: translation elongation factor 4
  • replicon: chromosome
  • strand: -
  • coordinates: 1128541..1130364
  • length: 1824
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_AP018936 (1128541..1130364) NCBI
  • BioCyc: EL277_RS05920 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1060 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAACTTAGAAGAATTGAAAAAACGACAGAGGAAGATCCGTAACTTCTCTATTATCGCC
    CATATTGACCACGGGAAATCAACTCTAGCAGACCGCATTTTGGAAAAAACAGAGACGGTT
    TCAAGTCGTGAAATGCAGGCCCAGCTTTTGGATAGCATGGATCTAGAGCGGGAACGTGGA
    ATTACTATTAAGTTGAATGCCATCGAGTTGAATTACACTGCAAAAGATGGGGAAACTTAT
    ATTTTCCACTTGATTGACACACCAGGGCACGTTGACTTTACCTATGAAGTTTCACGTTCG
    CTAGCTGCCTGTGAGGGTGCTATTTTGGTGGTCGATGCGGCTCAAGGAATTGAGGCTCAA
    ACTCTTGCCAACGTTTATCTGGCTTTGGATAATGATTTGGAAATCATGCCTATCATTAAT
    AAAATTGACCTGCCGGCTGCAGATCCGGAGCGCGTGCGTACAGAGATTGAAGATGTGATT
    GGTTTGGATGCCAGTGAAGCAGTTTTGGCTTCTGCCAAGGCTGGTATTGGGATTGAAGAA
    ATCCTCGAGCAAATTGTAGAAAAAGTACCAGCACCAACGGGTGATGTGACGGCGCCACTT
    AAGGCCTTGATTTTCGACTCTGTTTACGATGCTTACCGTGGGGTTATCCTCCAAGTGCGT
    GTCATGGACGGAGTGGTCAAACCTGGTGATAAGATTCAGCTCATGAGCAATAGTAAGACC
    TTTGATGTGGCCGAAGTCGGTATTTTTACACCAAAAGCGGTTGGTCGTGATTTCCTTGCG
    ACTGGTGATGTTGGTTACATTGCGGCGTCTATCAAGACGGTTCAGGATACTCGTGTGGGT
    GATACCGTTACCTTGGCAACCAATCCTGCGGCAGAACCATTACATGGTTATAAGCAGATG
    AATCCTATGGTCTTTGCGGGTCTCTACCCAATCGAATCAAACAAATACAATGACCTGCGT
    GAAGCGCTTGAAAAATTGCAACTGAATGATGCTAGTCTTCAGTTTGAACCAGAAACATCT
    CAGGCACTTGGATTTGGTTTCCGTTGTGGATTTCTTGGACTTCTCCATATGGATGTTATC
    CAGGAACGTTTAGAGCGTGAGTTCAATATTGACCTCATCATGACAGCTCCGTCTGTTATT
    TACAAAGTTAATTTGACCGACGGTGAGTCTATGGATGTGTCTAACCCATCTGAGTTTCCA
    GACCCAACTAAGATTGCGACCATTGAAGAGCCTTATGTCAAGGCGCAAATCATGGTACCA
    CAGGAGTTCGTCGGAGCAGTAATGGAGCTAGCTCAGCGTAAGCGTGGGGACTTTGTGACT
    ATGGACTATATTGATGATAACCGTGTCAATGTTATCTATCAAATTCCTCTTGCTGAAATT
    GTCTTTGACTTCTTTGATAAACTTAAGTCTTCGACACGTGGTTATGCAAGCTTTGACTAC
    GAATTGTCAGAATATCGCCCATCTAAGCTGGTGAAAATGGATATCCTTCTCAATGGAGAT
    AAGGTGGATGCCCTCAGCTTTATCGTTCACAAGGACTTTGCCTACGAACGTGGGAAACTC
    ATCGTTGATAAACTCAAGAAAATCATCCCTCGCCAACAATTTGAAGTTCCAATCCAAGCG
    GCTATTGGACACAAGATTGTCGCTCGTACTGATATCAAGGCCCTTCGTAAGAACGTACTT
    GCTAAATGTTATGGTGGTGACGTTTCTCGTAAGCGTAAACTCCTTGAAAAACAAAAAGCT
    GGTAAGAAACGCATGAAATCCATCGGATCAGTTGAAGTTCCGCAAGAAGCCTTCCTCAGC
    GTCTTGAGTATGGATGAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1824

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EL277_RS05920 [old locus tag: SPNU_1124 ]
  • symbol: LepA
  • description: translation elongation factor 4
  • length: 607
  • theoretical pI: 4.87609
  • theoretical MW: 67707.4
  • GRAVY: -0.143493

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.5.n1?  ExPASy
    Elongation factor 4 GTP + H2O = GDP + phosphate
  • TIGRFAM:
    Unknown function General elongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 1041.1)
    and 18 more
    Cellular processes Cellular processes Adaptations to atypical conditions GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 213.8)
    Genetic information processing Protein synthesis Translation factors GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 213.8)
    Signal transduction Regulatory functions Other GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 213.8)
    Genetic information processing Protein synthesis Translation factors translation elongation factor G (TIGR00484; HMM-score: 189.1)
    Genetic information processing Protein synthesis Translation factors translation elongation factor aEF-2 (TIGR00490; HMM-score: 169.7)
    Genetic information processing Protein synthesis Translation factors peptide chain release factor 3 (TIGR00503; HMM-score: 101.3)
    Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 79.9)
    Genetic information processing Protein synthesis Translation factors translation elongation factor Tu (TIGR00485; HMM-score: 73.1)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 69.3)
    Genetic information processing Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 68.8)
    Genetic information processing Protein synthesis Translation factors translation elongation factor EF-1, subunit alpha (TIGR00483; HMM-score: 56.6)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfate adenylyltransferase, large subunit (TIGR02034; EC 2.7.7.4; HMM-score: 53.2)
    translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 36.7)
    Genetic information processing Protein synthesis Translation factors translation initiation factor aIF-2 (TIGR00491; HMM-score: 34)
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 31.8)
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 17.5)
    Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 16.8)
    Metabolism Energy metabolism Amino acids and amines ethanolamine utilization protein, EutP (TIGR02528; HMM-score: 15.7)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    P-loop_NTPase (CL0023) GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 179.3)
    no clan defined LepA_C; GTP-binding protein LepA C-terminus (PF06421; HMM-score: 169.6)
    and 8 more
    EF-G_C (CL0437) EFG_C; Elongation factor G C-terminus (PF00679; HMM-score: 88.6)
    EFTPs (CL0575) GTP_EFTU_D2; Elongation factor Tu domain 2 (PF03144; HMM-score: 34)
    EF-G_C (CL0437) EFG_III; Elongation Factor G, domain III (PF14492; HMM-score: 26.7)
    RF3_C; Class II release factor RF3, C-terminal domain (PF16658; HMM-score: 23.6)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 22)
    Ras; Ras family (PF00071; HMM-score: 15.2)
    SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 13.6)
    no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003518
    • TAT(Tat/SPI): 0.00032
    • LIPO(Sec/SPII): 0.000369
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001047222 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNLEELKKRQRKIRNFSIIAHIDHGKSTLADRILEKTETVSSREMQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVIGLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQVRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQDTRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQFEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMDVSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVNVIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFIVHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGGDVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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