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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 14-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae ASP0581
  • locus tag: EL334_RS02000 [old locus tag: ASP0581_03710 ]
  • pan locus tag?: PNEUPAN001202000
  • symbol: EL334_RS02000
  • pan gene symbol?: eng
  • synonym:
  • product: SpGH101 family endo-alpha-N-acetylgalactosaminidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EL334_RS02000 [old locus tag: ASP0581_03710 ]
  • symbol: EL334_RS02000
  • product: SpGH101 family endo-alpha-N-acetylgalactosaminidase
  • replicon: chromosome
  • strand: +
  • coordinates: 355189..360477
  • length: 5289
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NZ_AP019192 (355189..360477) NCBI
  • BioCyc: EL334_RS02000 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0335 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAATAAAGGATTATTTGAAAAACGTTGTAAATATAGTATTCGGAAATTTTCATTAGGT
    GTTGCTTCTGTTATGATTGGAGCTGCATTCTTTGGGACAAGTCCGGTTCTTGCAGATAGC
    GTGCAGTCTGGTTCCACGGCGAACTTACCAGCTGATTTAGCTACTGCTCTTGCAACAGCA
    AAAGAGAATGATGGGCGTGATTTTGAAGCGCCTAAGGTGGGAGAAGACCAAGGTTCTCCA
    GAAGTTACAGATGGACCTAAGACAGAAGAAGAACTATTAGCACTTGAAAAAGAAAAACCG
    GCTGAAGAAAAACCAAAAGAGGATAAACCTGAAACACCTAAGACGGTAACTCCTGAATGG
    CAAACGGTAGAGAAAAAAGAACAACAGGGAACAGTCACTATCCGAGAAGAAAAAGGTGTC
    CGCTACAACCAACTATCCTCAACTGCTCAAAATGATAACGCAGGCAAACCAGCCCTGTTT
    GAAAAGAAGGGCTTGACCGTTGATGCCAATGGAAATGCAACTGTTGATTTAACCTTCAAA
    GATGATTCTGAAAAGGGCAAATCACGCTTTGGTGTCTTCCTGAAATTTAAAGATACCAAT
    AATAATGTTTTTGTCGGTTATGACAAGGATGGCTGGTTCTGGGAATACAAAACTCCAGGT
    AATAGCACATGGTATAAAGGCAACCGTGTAGTTGCACCAGAACCGGGTTCTGTAAACCGT
    CTTTCTATCACTCTCAAGTCAGATGGACAACTCAATGCCAGCAATAACGATGTCAATCTC
    TTTGACACAGTGACACTGCCTGGGGCGGTCAATGACCATCTTAAAAATGAGAAGAAGATC
    CTTCTCAAGGCAGGAACTTATGGCAATGACCGTACGGTTGTCAGCGTTAAAACAGACAAT
    CAAGAAGGCGTAAAAGCGGATGATACTCCTGCCCAGAAAGAAACAGGTCCGGTTGTTGAC
    GATAGCAAGGTGACTTATGATACGATCCAGTCTAAGGTACTTAAGGCAGTAATTGACCAA
    GCCTTCCCTCGTGTCAAGGAATACACCTTGAATGGACATACTTTGCCAGGACAGGTGCAA
    CAGTTCAACCAAGTCTTTATCAATAACCACCGAATCACCCCTGAAGTCACTTATAAGAAA
    ATCAATAAGACAACAGCAGAGTACTTGATGAAGATTCGCGATGATGCTCACTTAATCAAT
    GCGGAAATGACAGTACGCTTGCAAGTTGTGGACAATCAATTGCACTTTGATGTGACCAAG
    ATTGTCAACCACAATCAAGTCACTCCAGGTCAAAAGATTGATGACGAAAGAAAACTACTT
    TCTTCTATTAGTTTCCTCGGCAATGCTTTAGTCTCTGTTTCTAGTGATCAAACTGGTGCT
    AAGTTTGATGGGGCAACTATGTCAAACAATACGCATGTCAGTGGAGATGATCATATCGAT
    GTAACCAATCCAATGAAAGACCTAGCCAAGGGTTACATGTATGGATTTGTTTCTACAGAT
    AAGCTTGCTGCTGGTGTTTGGAGTAACTCTCAAAACAGCTATGGTGGTGGTTCGAATGAC
    TGGACTCGTTTGACAGCTTATAAAGAAACAGTCGGAAATGCCAACTATGTAGGAATCCAC
    AGCTCTGAATGGCAATGGGAAAAAGCTTATAAGGGCATTGTTTTCCCAGAATACACGAAG
    GAACTTCCGAGTGCTAAGGTTGTTATCACTGAAGATGCCAATGCAGACAAGAAAGTTGAT
    TGGCAAGATGGTGCCATTGCTTATCGTAGCATTATGAACAACCCTCAAGGTTGGGAAAAA
    GTTAAGGATATCACAGCTTACCGTATCGCGATGAACTTTGGTTCTCAAGCACAAAACCCA
    TTCCTTATGACCTTGGATGGTATCAAGAAAATCAATCTCCATACAGATGGTCTTGGGCAA
    GGTGTTCTCCTTAAAGGATATGGTAGCGAAGGCCATGACTCTGGTCACTTGAACTATGCT
    GATATTGGTAAGCGTATCGGTGGTGTCGAAGACTTCAAGACCCTAATTGAGAAGGCTAAG
    AAATATGGAGCTCATCTAGGTATCCACGTTAACGCTTCAGAAACTTATCCTGAGTCTAAA
    TACTTCAATGAAAAAATTCTCCGTAAGAATCCAGATGGAAGCTATAGCTATGGTTGGAAC
    TGGCTAGATCAAGGTATCAACATTGATGCTGCCTATGACCTAGCTCATGGTCGTTTGGCA
    CGTTGGGAAGATTTGAAGAAAAAACTTGGTGACGGTCTCGACTTTATCTATGTGGACGTT
    TGGGGTAATGGTCAATCAGGTGATAACGGTGCCTGGGCTACCCACGTTCTTGCTAAAGAA
    ATTAACAAACAAGGCTGGCGCTTTGCGATCGAGTGGGGCCATGGTGGTGAGTACGACTCT
    ACCTTCCATCACTGGGCAGCTGACTTGACCTACGGTGGCTACACCAATAAAGGTATCAAC
    AGTGCCATCACCCGCTTTATCCGTAACCACCAAAAAGATGCTTGGGTAGGGGACTACAGA
    AGTTATGGTGGTGCAGCCAACTATCCACTGCTAGGTGGCTACAGCATGAAAGACTTTGAA
    GGCTGGCAAGGACGGAGCGACTACAATGGCTACGTGACTAACCTCTTTGCCCATGATGTC
    ATGACCAAGTACTTCCAACACTTCACTGTAAGTAAATGGGAAAATGGTACACCGGTGACT
    ATGACCGATAACGGTAGCACCTATAAATGGACTCCAGAAATGCGAGTGGAATTGGTAGAT
    GCTGACAATAATAAAGTAGTTGTAACTCGTAAGTCAAATGATGTCAATAGCCCACAATAT
    CGCGAACGTACAGTAACGCTCAACGGACGTGTCATCCAAGATGGTTCAGCTTACTTGACT
    CCTTGGAACTGGGATGCAAATGGTAAGAAACTTTCTACTGATAAGGAAAAAATGTACTAC
    TTCAATACGCAGGCTGGTGCAACAACTTGGACCCTTCCAAGCGATTGGGCAAAGAGCAAG
    GTTTACCTTTACAAGCTAACTGACCAAGGTAAGACAGAAGAGCAAGAACTAACTGTAAAA
    GATGGTAAAATTACCCTAGATCTTCTAGCAAATCAACCATACGTACTTTACCGTTCAAAA
    CAAACCAATCCTGAAATGTCATGGAGTGAAGGCATGCACATCTATGACCAAGGATTTAAT
    AGTGGAACCTTGAAACACTGGACCATTTCAGGCGATGCTTCTAAGGCAGAAATTGTCAAG
    TCTCAAGGGGCAAACGATATGCTTCGTATTCAAGGAAACAAAGAAAAAGTTAGTCTCACT
    CAGAAATTAACTGGCTTGAAACCAAATACCAAGTATGCCGTTTATGTCGGTGTCGATAAC
    CGTAGTAATGCCAAGGCAAGTATCACTGTGAATACTGGTGAAAAAGAAGTGACTACTTAT
    ACCAATAAGTCTCTCGCCCTCAACTATGTAAAAGCCTACGCCCACAATACACGTCGTAAC
    AATGCTACAGTTGACGATACAAGTTACTTCCAAAACATGTACGCCTTCTTTACAACTGGA
    TCGGACGTCTCAAATGTTACTCTGACATTGAGTCGTGAAGCTGGTGATCAAGCAACTTAC
    TTTGATGAAATTCGTACCTTTGAAAACAATTCAAGCATGTACGGAGACAAGCATGATACA
    GGTAAAGGCACCTTCAAGCAAGACTTTGAAAATGTTGCTCAGGGTATCTTCCCATTTGTA
    GTGGGTGGTGTTGAAGGTGTTGAAGATAACCGCACTCACTTGTCTGAAAAACACGATCCA
    TATACACAACGTGGTTGGAATGGTAAGAAAGTCGATGATGTTATCGAAGGAAATTGGTCA
    CTCAAGACAAATGGACTAGTGAGCCGTCGTAACTTGGTTTACCAAACTATTCCGCAAAAC
    TTCCGTTTTGAAGCAGGTAAGACCTACCGTGTAACCTTTGAATACGAAGCAGGTTCAGAC
    AATACCTATGCTTTTGTAGTCGGTAAGGGAGAATTCCAGTCAGGTCGTCGTGGCACTCAA
    GCAAGCAACTTGGAAATGCATGAATTGCCAAATACTTGGACAGATTCTAAGAAAGCTAAG
    AAGGCAACCTTCCTTGTGACAGGTGCAGAAACAGGGGATACTTGGGTAGGTATCTACTCA
    ACTGGCAATGCAAGTAATACTCGTGGTGATTCTGGTGGAAATGCCAACTTCCGTGGTTAT
    AACGACTTCATGATGGATAATCTTCAAATCGAAGAAATTACCCTAACAGGTAAGATGTTG
    ACAGAAAATGCTCTGAAGAACTACTTGCCAACGGTTGCCATGACTAACTACACCAAAGAG
    TCTATGGATGCTTTGAAAGAGGCGGTCTTTAACCTCAGTCAGGCCGATGATGATATCAGT
    GTGGAAGAAGCGCGTGCAGAGATTGCCAAGATTGAAGCCTTGAAGAATGCTTTGGTTCAG
    AAGAAAATGGCTTTGGTAGCAGAAGACTTTGAAAGTTTGGATGCGCCAGCTCAACCAGGT
    GAAGGCCTAGAGAATGCTTTTGATGGCAATGTGTCTAGTCTATGGCATACATCTTGGAAT
    GGTGGAGATGTAGGCAAGCCTGCAACCATGGTCTTGAAAGAACCAACTGAAATCACTGGA
    CTTCGTTACGTTCCACGTGGATCGGGTTCAAATGGTAACTTGCGTGATGTGAAACTTGTT
    GTGACAGATGAGTCTGGCAAGGAGCATACCTTTACTGCAACTGATTGGCCAGATAACAAT
    AAGCCAAAAGACATTGATTTTGGTAAGACAATTAAGGCTAAGAAAATTGTCCTTACAGGT
    ACTAAGACTTACGGAGATGGTGGCGATAAATACCAATCTGCAGCGGAACTCATCTTTACT
    CGTCCACAGGTAGCAGAAACACCTCTTGACTTGTCAGGCTATGAAGCAGCTTTGGCTAAG
    GCTCAGAAATTAACAGACAAAGACAATCAAGAGGAAGTAGCTAGCGTTCAGGCAAGCATG
    AAATATGCGACGAATAACCATCTCTTGACGGAAAGAATGGTGGAATACTTTGCAGATTAT
    CTCAACCAATTAAAAGATTCTGCTACGAAACCAGATGCTCCAACTGTAGAGAAACCTGAG
    TTTAAACTTAGATCTTTAGCTTCCGATCAAGGTAAGACGCCAGATTATAAGCAAGAAATA
    GCTAGACCAGAAACACCTGAACAAATCTTGCCAACAACAGGTGAGAGTCAATCTGACACA
    GCCCTCTTCCTAGCAGGTGTTAGCCTAGCCCTATCTGCTCTCTTTGTAGTAAAAACGAAG
    AAAGACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
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    1260
    1320
    1380
    1440
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    1560
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    1800
    1860
    1920
    1980
    2040
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    2160
    2220
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    2760
    2820
    2880
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    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3900
    3960
    4020
    4080
    4140
    4200
    4260
    4320
    4380
    4440
    4500
    4560
    4620
    4680
    4740
    4800
    4860
    4920
    4980
    5040
    5100
    5160
    5220
    5280
    5289

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EL334_RS02000 [old locus tag: ASP0581_03710 ]
  • symbol: EL334_RS02000
  • description: SpGH101 family endo-alpha-N-acetylgalactosaminidase
  • length: 1762
  • theoretical pI: 6.03374
  • theoretical MW: 195768
  • GRAVY: -0.668842

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.97?  ExPASy
    Endo-alpha-N-acetylgalactosaminidase 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O = 3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 54.7)
    and 1 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 20)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    Glyco_hydro_tim (CL0058) Glyco_hydro_101; Endo-alpha-N-acetylgalactosaminidase (PF12905; HMM-score: 413.7)
    and 8 more
    Concanavalin (CL0004) GalBD_like; Galactose-binding domain-like (PF17974; HMM-score: 296.6)
    Gal_mutarotase (CL0103) Gal_mutarotas_3; Galactose mutarotase-like fold domain (PF18080; HMM-score: 296.4)
    GBD (CL0202) GH101_N; Endo-alpha-N-acetylgalactosaminidase N-terminal (PF17995; HMM-score: 274.5)
    GHD (CL0369) Glyco_hyd_101C; Glycosyl hydrolase 101 beta sandwich domain (PF17451; HMM-score: 145.3)
    GBD (CL0202) F5_F8_type_C; F5/8 type C domain (PF00754; HMM-score: 47.9)
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 46.1)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 16.7)
    WW (CL0680) WW; WW domain (PF00397; HMM-score: 15.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helix: 1
  • DeepLocPro: Cell wall & surface
    • Cytoplasmic Score: 0.0004
    • Cytoplasmic Membrane Score: 0.0134
    • Cell wall & surface Score: 0.6944
    • Extracellular Score: 0.2918
  • SignalP: Signal peptide SP(Sec/SPI) length 38 aa
    • SP(Sec/SPI): 0.982143
    • TAT(Tat/SPI): 0.013699
    • LIPO(Sec/SPII): 0.001381
    • Cleavage Site: CS pos: 38-39. VLA-DS. Pr: 0.9461
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_033705366 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSGSTANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAEEKPKEDKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAGKPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWFWEYKTPGNSTWYKGNRVVAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPGAVNDHLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTIQSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINKTTAEYLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISFLGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLAAGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKELPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQNPFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIEKAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLAHGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWGHGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLLGGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYKWTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDANGKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKITLDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQGANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYTNKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQATYFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEKHDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEYEAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGDTWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPTVAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKMALVAEDFESLDAPAQPGEGLENAFDGNVSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRGSGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYGDGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYATNNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLRSLASDQGKTPDYKQEIARPETPEQILPTTGESQSDTALFLAGVSLALSALFVVKTKKD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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