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serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
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AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 15-DEC-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae ASP0581
  • locus tag: EL334_RS07775 [old locus tag: ASP0581_14690 ]
  • pan locus tag?: PNEUPAN002851000
  • symbol: EL334_RS07775
  • pan gene symbol?: metB
  • synonym:
  • product: cystathionine gamma-synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EL334_RS07775 [old locus tag: ASP0581_14690 ]
  • symbol: EL334_RS07775
  • product: cystathionine gamma-synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1450259..1451353
  • length: 1095
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_AP019192 (1450259..1451353) NCBI
  • BioCyc: EL334_RS07775 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1353 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGCAAGGAATTACACATTAACACAATTTTAGCACAAGCAGGGATCAAGTCTGATGAG
    GCAACAGGTGCTTTGGTGACACCACTTCATTTTTCAACGACCTATCAGCATCCAGAGTTT
    GGTCGATCTACTGGGTTTGACTATACGCGCACTAAAAATCCAACTCGTAGTAAGGCTGAG
    GAAGTCTTGGCGGCTATTGAGTCAGCAGACTATGCCCTAGCGACTAGCTCAGGGATGTCA
    GCTATTGTACTGGCCTTTAGCGTCTTTCCAGTAGGAAGTAAGGTCTTGGCAGTGCGTGAT
    CTTTACGGTGGTTCTTTTCGCTGGTTTAACCAAGTGGAGCAGGAAGGTCATTTCCATTTT
    ACCTATGCCAATACAGAAGAAGAGTTGATTGCCGAGTTAGAAAAGGATGTGGATGTTCTC
    TATATCGAAACCCCAACCAATCCCTTGATGTTGGAATTTGATATCGAAAAACTAGCAAAA
    TTGGCTCATGCTAAGGGTTCCAAAGTGGTGGTGGACAATACCTTCTATAGCCCTATTTAC
    CAACGTCCGATTGAAGATGGAGCAGATATCGTTCTCCATTCAGCAACCAAGTATCTAGCA
    GGCCACAATGATGTCTTGGCTGGAGTGGTTGTGACCAATAGTTTAGAACTATACGAGAAG
    CTTTTTTACAATCTTAATACAACAGGGGCAGTCTTGTCTCCATTTGACAGCTACCAGTTG
    CTTCGTGGTCTCAAGACCTTGTCTCTTCGTATGGAGCGCTCAACAGCTAACGCCCAAGAA
    GTGGTTGCCTTTTTGAAGGATTCTCCAGCAGTTAAGGAAGTTCTCTACACTGGTCGTGGA
    GGCATGATTTCCTTTAAAGTAGCCGATGAAACACGCATTCCTCATATTTTGAACAGTCTC
    AAGGTCTTCTCTTTTGCGGAAAGTTTGGGCGGAGTGGAAAGTCTTATTACTTATCCAACG
    ACTCAAACTCATGCTGATATTCCAGCAGAAGTACGCCATTCTTATGGTTTGACAGATGAC
    CTCTTGCGTTTGTCTATTGGGATTGAGGATGCTAGAGATTTGATTGCAGATTTGCGCCAA
    GCCTTAGAAGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1095

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EL334_RS07775 [old locus tag: ASP0581_14690 ]
  • symbol: EL334_RS07775
  • description: cystathionine gamma-synthase
  • length: 364
  • theoretical pI: 4.93614
  • theoretical MW: 40119.1
  • GRAVY: -0.0717033

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.48?  ExPASy
    Cystathionine gamma-synthase O4-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 365.4)
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 310)
    Metabolism Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 297.6)
    and 9 more
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 268.3)
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 241.6)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 241.6)
    Metabolism Amino acid biosynthesis Aspartate family cystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 157.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 19.5)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01976; HMM-score: 16.3)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 14.7)
    2-aminoethylphosphonate aminotransferase (TIGR03301; EC 2.6.1.-; HMM-score: 13.4)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 11.4)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    PLP_aminotran (CL0061) Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 393.3)
    and 4 more
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 15.5)
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 14.4)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 12.5)
    Enolase_TIM (CL0256) MR_MLE_C; Enolase C-terminal domain-like (PF13378; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013988
    • TAT(Tat/SPI): 0.001737
    • LIPO(Sec/SPII): 0.002468
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000031965 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRSTGFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLYGGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAKLAHAKGSKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELYEKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLYTGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHSYGLTDDLLRLSIGIEDARDLIADLRQALEG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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