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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 09-MAY-2015

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae NCTC7465
  • locus tag: ERS445053_00946 [new locus tag: AT689_RS04845 ]
  • pan locus tag?: PNEUPAN003369000
  • symbol: dpnA
  • pan gene symbol?: dpnA
  • synonym:
  • product: DNA (cytosine-5-)-methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: ERS445053_00946 [new locus tag: AT689_RS04845 ]
  • symbol: dpnA
  • product: DNA (cytosine-5-)-methyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 893558..894328
  • length: 771
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: LN831051 (893558..894328) NCBI
  • BioCyc: see AT689_RS04845
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACAAAACCATACTACAATAAAAATAAGATGATTCTTGTTCATTCAGATACGTTCAAG
    TTCTTATCAAAAATGAAACCAGAAAGTATGGATATGATTTTTGCTGATCCACCTTATTTT
    TTAAGTAATGGTGGAATATCTAATTCTGGGGGACAAGTTGTTTCTGTTGATAAAGGAGAT
    TGGGATAAAATTTCTTCATTCGAAGAAAAACATGAGTTTAATCGTAAATGGATTCGCCTA
    GCAAAAGAAGTTCTGAAGCCTAATGGGACGGTATGGATTTCAGGTAGTTTGCACAACATA
    TACTCAGTTGGAATGGCATTAGAACAAGAAGGTTTTAAAATTCTGAATAATATTACTTGG
    CAGAAAACAAACCCTGCCCCCAATTTATCTTGTCGTTATTTTACCCATTCTACTGAAACC
    ATTTTATGGGCCAGAAAAAATGATAAAAAAGCTCGTCATTACTACAATTATGATTTAATG
    AAAGAATTGAATGATGGAAAACAAATGAAAGATGTCTGGACGGGTTCTTTAACAAAGAAA
    GTTGAAAAATGGGCTGGGAAACATCCAACTCAAAAACCAGAGTATTTGTTAGAACGCATT
    ATTTTAGCCTCTACTAAAGAGGGTGACTATATTCTAGACCCATTTGTTGGTAGTGGCACT
    ACGGGTGTTGTTGCGAAGCGGTTAGATAGAAGATTTATAGGTATCGATGCTGAAAAAGAA
    TATTTAAAAATTGCAAGGAAGAGGTTGGAGGCAGAAAATGAAACAAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    771

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: ERS445053_00946 [new locus tag: AT689_RS04845 ]
  • symbol: DpnA
  • description: DNA (cytosine-5-)-methyltransferase
  • length: 256
  • theoretical pI: 9.85706
  • theoretical MW: 29582.7
  • GRAVY: -0.675781

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.72?  ExPASy
    Site-specific DNA-methyltransferase (adenine-specific) S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
  • TIGRFAM:
    Hypothetical proteins Conserved putative methyltransferase, TIGR01177 family (TIGR01177; HMM-score: 23)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 20.1)
    and 3 more
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 15.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA adenine methylase (TIGR00571; EC 2.1.1.72; HMM-score: 14.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD (TIGR00095; EC 2.1.1.171; HMM-score: 11.6)
  • TheSEED: data available for Hungary19A-6
  • PFAM:
    NADP_Rossmann (CL0063) N6_N4_Mtase; DNA methylase (PF01555; HMM-score: 193.4)
    and 10 more
    Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 23.3)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 21.3)
    Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 20.3)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 20.1)
    no clan defined DUF4136; Domain of unknown function (DUF4136) (PF13590; HMM-score: 19.1)
    NADP_Rossmann (CL0063) Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 18.3)
    N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 17.7)
    MethyltransfD12; D12 class N6 adenine-specific DNA methyltransferase (PF02086; HMM-score: 15.8)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 15.5)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9715
    • Cytoplasmic Membrane Score: 0.0023
    • Cell wall & surface Score: 0.0012
    • Extracellular Score: 0.025
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009798
    • TAT(Tat/SPI): 0.000223
    • LIPO(Sec/SPII): 0.000493
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: COT15253 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNGGISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYSVGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLMKELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGSGTTGVVAKRLDRRFIGIDAEKEYLKIARKRLEAENETN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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