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serotype 1

NCBI: 04-FEB-2016

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae INV104
  • locus tag: INV104_05960 [new locus tag: INV104_RS03495 ]
  • pan locus tag?: PNEUPAN001639000
  • symbol: thiM
  • pan gene symbol?: thiM-1
  • synonym:
  • product: hydroxyethylthiazole kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: INV104_05960 [new locus tag: INV104_RS03495 ]
  • symbol: thiM
  • product: hydroxyethylthiazole kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 672465..673247
  • length: 783
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACAAGTTTAAAATTATTAAAAGAAAAAGCACCATTGGTCATTTGTATAACCAATGAT
    GTAGTAAAAAATTTCACAGCAAATGGATTAGTAGCACTGGGCGCCTCACCAGCCATGAGT
    GAGTTTCCAGCAGATTTAGAGGATTTGTTAAAGTATGCTGGTGGTTTATTAATAAACATA
    GGAACATTGACAGATGAAAATTGGAAATTATACCAAGCTGCTCTGAAAATTGCAGAGAAA
    TATAATGTCCCAGCAGTTTTAGATCCTGTAGCCTGTGGAGCAGGAGAATATAGAAAAAAA
    GTAGCAGATGATCTAATCAACAATTATAAACTAGCAGCGATTAGAGGAAATGCTGGCGAG
    ATTGCCTCTTTAGTAGGAATAGATGTGGCATCTAAAGGAGTAGATAGTGCGGGCGTAGAT
    AATATTGACGAAATTGCTCTAGCAGCAAATGAGAAGTTCAATATTCCAATAGTAGTAACA
    GGTGAAGTGGATGCCATTGCTGTTAATGGAGAAGTGGTAATGATTCATAATGGTAGTGCT
    ATGATGCCGAAAGTCATTGGGACAGGATGCTTATTAGGAGCTGTAGTAGCAAGCTTTATC
    GGACTAGAAAAAGGTCAAGAATTGAAATCATTAGAAACAGCAATGTTGGTTTACAATATC
    GCTGGAGAAATGGCAGAAAAACGTCCAAATGGACATCTTCCTGGGACATTTAAAGTTGAA
    TTTATAAATGCCTTATACGAGATTACAGATGAAGATGTAAAGGAATTCAAAAGAGTGAAG
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    783

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: INV104_05960 [new locus tag: INV104_RS03495 ]
  • symbol: ThiM
  • description: hydroxyethylthiazole kinase
  • length: 260
  • theoretical pI: 4.61534
  • theoretical MW: 27636.9
  • GRAVY: 0.215385

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.50?  ExPASy
    Hydroxyethylthiazole kinase ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxyethylthiazole kinase (TIGR00694; EC 2.7.1.50; HMM-score: 266.7)
  • TheSEED  :
    • Hydroxyethylthiazole kinase (EC 2.7.1.50)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  Hydroxyethylthiazole kinase (EC 2.7.1.50)
  • PFAM:
    Ribokinase (CL0118) HK; Hydroxyethylthiazole kinase family (PF02110; HMM-score: 263.7)
    and 2 more
    Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 19.8)
    Carb_kinase; Carbohydrate kinase (PF01256; HMM-score: 16.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9611
    • Cytoplasmic Membrane Score: 0.0023
    • Cell wall & surface Score: 0.0006
    • Extracellular Score: 0.036
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.05344
    • TAT(Tat/SPI): 0.00684
    • LIPO(Sec/SPII): 0.001809
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CBW36279 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPADLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVADDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVTGEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVYNIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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