From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 14-DEC-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae MDRSPN001
  • locus tag: MDRSPN_RS02455 [old locus tag: MDRSPN_00457 ]
  • pan locus tag?: PNEUPAN003144000
  • symbol: MDRSPN_RS02455
  • pan gene symbol?: priA
  • synonym:
  • product: primosomal protein N'

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: MDRSPN_RS02455 [old locus tag: MDRSPN_00457 ]
  • symbol: MDRSPN_RS02455
  • product: primosomal protein N'
  • replicon: chromosome
  • strand: +
  • coordinates: 463044..465440
  • length: 2397
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_AP018391 (463044..465440) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1546 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGCCCTAGCTAAGATTATCGTAGATGTGCCCTTGATGCAGACGGACCAGCCCTATAGT
    TACAGGATTCCGGAGGAATTTGAGGGAATGCTGGAAGTTGGGATGCGGGTTCATGTGCCT
    TTTGGTAAGGGTAATCGCCTGATTCAAGGGATTGTTCTTGGTTTGGAGTCCCAATCAGAT
    GGAGAAGAGATGGAGCAAGATTTAAAAGATATTGCAGAGGTGCTGGATTTTTCTCCTGTT
    CTCACGCCAGAACAACTCTGGCTGGCTGAGGAGTTACGCAAATCTGTCTTCTCCTACAAA
    ATTTCTATCCTCAAGGCCATGCTTCCAGGATTTCTGAATTCTAGCTATGACAAGATTCTC
    TATCCTCTGGAAGGCTTGAGTCAGGAAGAACGAGTGCGCCTGTTTGGTTCAGAAGATTCG
    CTAGCCTTTTCTTCACTAGACCTTGCCAAGCAAGCTGAAATGATGCGTTTGACTAGAAAA
    GGCCTGCTTGTTCTGGAATATCAGGCAGTCGATCAAAAGAAGGTCAAGACCCAGTCTTGG
    TATGAGGTTGACCATGCTCAATTAGAAGGTGTTGAGATTTCTACACGTGCCAAGAAAAAG
    TTGGAACTGAGAGATTATCTGCTGTCTCATCCAGAGAGCGCTTCCTTGGCTAGCTTGTTA
    GAGTCCTACTCGCGAGAGCAAGTCAACTTCTTTGTGGATCAAGGTGCTGTTACCATAGTC
    CAAAAGGAAGTTCAACGCTCGGCTGCTTATTTTGAAGGAATTGAAGCAAGTAGACCTTTG
    GAGTTAAATCCAGAACAAAGACAGGCGCGTGATGCGGTTGTGAGTTCTATTGGCAGTTCT
    CAACCTCCCTTTCTACTTCAAGGGATTACAGGAAGTGGGAAGACCGAGGTTTACTTGCAG
    ATTATCCAAGGTGCCCTGGATAAGGGCAAGACAGCTATTTTGCTGGTACCTGAGATTTCC
    TTGACTCCTCAGATGACCGAGCGTTTTATAGCACGTTTTGGGGACAAGGTAGCCATTCTT
    CACTCAGGATTATCCAATGGTGAAAAGTATGATGAATGGCGCAAGGTGGAGCGTGGCGAT
    GCCCAAGTTGTTGTTGGTGCCAGATCTGCTATCTTTGCTCCTCTGAAAAATCTGGGTGTC
    ATGATTATTGATGAAGAGCATGAAGCGGCTTATAAGCAGGACAGCAATCCCCGTTACCAT
    GCCAGAGAGGTAGCTATTTTACGGGCTCAGTATAATCAAGCAACTCTGGTACTTGGATCT
    GCAACGCCGAGCTTAGAGAGCCGGGCACGTGCTGGCAAAGGCGTCTATCAACACTTACGT
    CTAACCCAACGTGCCAATCCTTTGGCTACAATCCCTGAGGTTCAAGTGATTGACTTTCGA
    GACTATATCGGACAAAATGAGACGTCAAACTTTACGCCTCCTTTGCTAGAGGCTATCCAA
    GACCGTCTGGTTAAAAAAGAGCAGGTGGTTCTCATGCTCAATCGCCGTGGTTATTCTAGC
    TTTGTCATGTGTCGGGAGTGTGGGACGGTGGATACTTGTCCCAACTGCGATATTTCTCTG
    ACCTTGCATATGGATACCAAGACTATGAACTGCCATTATTGTGGTTTCTCGAAGGATATT
    CCTCAGGTCTGTCCTAACTGTAAGAGCCGCAGTATTCGTTACTATGGGACGGGAACTCAG
    AAGGCTTATGATGAGCTAGCAGAACTCTTTCCCCAAGCTCGCATTTTGAGGATGGATGTG
    GATACGACTCGAAAGAAAGGCAGTCACCAAGCCTTGCTTGACCAGTTTGGGCGAGGGGAA
    GCGGATATTTTACTTGGTACTCAGATGATTGCAAAGGGTTTGGATTTTCCCAATGTTACC
    CTAGTCGGAGTTCTAAATGCGGATACGGCCTTGAATCTGCCTGATTTCCGTTCTTCTGAG
    AGAACCTTCCAGCTCTTGACTCAGGTGGCAGGTCGAGCAGGTCGGGCTGAAAAAGCAGGT
    CAAGTCTTGATACAGTCCTACAATTCGCAGCACTATGCTATTCGATTTGCCAAGGATCAA
    GATTACGAAGGCTTTTATGCCTATGAAATGGGAATCAGACGACAACTCGGCTATCCACCT
    TACTATTTCACGATTGGTATTACCCTTTCTCACAAGAAAGAAGAAGAGGTTTTCAAACGT
    GCCTATGAAGTCATGAACATTTTGCGGTCAGGCTTGTCAGAGACCAGTCCCATTCTGGGG
    CCAACGCCAAAACCGATTGCCCGTACCCACAACCTCTATCATTACCAGATTTTAATTAAA
    TACCGTTTAGAAGATGAGCTGGGGCCGACCCTCAACCAGGTCTTGGCCTTGACTCAAGAA
    CGGGAAAATAGTGAGCTCCGTCTCAGCATTGACCATGAGCCGCAGCAATTTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2397

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: MDRSPN_RS02455 [old locus tag: MDRSPN_00457 ]
  • symbol: MDRSPN_RS02455
  • description: primosomal protein N'
  • length: 798
  • theoretical pI: 6.16193
  • theoretical MW: 90055.2
  • GRAVY: -0.324436

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 657.8)
    and 7 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 73.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 63.8)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 43)
    CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 19.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 17.5)
    CRISPR-associated helicase Cas3, subtype CYANO (TIGR03158; HMM-score: 14.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair reverse gyrase (TIGR01054; EC 3.6.4.12,5.99.1.3; HMM-score: 13.4)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    no clan defined PriA_3primeBD; 3'DNA-binding domain (3'BD) (PF17764; HMM-score: 75.8)
    P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 66.5)
    no clan defined PriA_C; Primosomal protein N C-terminal domain (PF18074; HMM-score: 66.5)
    and 11 more
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 50.5)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 46.6)
    Zn_Beta_Ribbon (CL0167) PriA_CRR; PriA DNA helicase Cys-rich region (CRR) domain (PF18319; HMM-score: 42.5)
    P-loop_NTPase (CL0023) AAA_30; AAA domain (PF13604; HMM-score: 14.8)
    no clan defined zf-CHY; CHY zinc finger (PF05495; HMM-score: 13.6)
    P-loop_NTPase (CL0023) TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 13.1)
    Zn_Beta_Ribbon (CL0167) zinc-ribbons_6; zinc-ribbons (PF07191; HMM-score: 12.5)
    P-loop_NTPase (CL0023) DUF2075; Schlafen group 3, DNA/RNA helicase domain (PF09848; HMM-score: 12.3)
    Zn_Beta_Ribbon (CL0167) Zn_Tnp_IS1595; Transposase zinc-ribbon domain (PF12760; HMM-score: 9.3)
    OrfB_Zn_ribbon; Putative transposase DNA-binding domain (PF07282; HMM-score: 7.6)
    DZR; Double zinc ribbon (PF12773; HMM-score: 7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00128
    • TAT(Tat/SPI): 0.000109
    • LIPO(Sec/SPII): 0.000161
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001152098 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKGNRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKISILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRKGLLVLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLASLLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSSIGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFGDKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYKQDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLATIPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDYEGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=MDRSPN_RS02455&oldid=105843"