PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 14-DEC-2020

⊟Summary

• organism: Streptococcus pneumoniae MDRSPN001
• locus tag: MDRSPN_RS08390 [old locus tag: MDRSPN_01599 ]
• pan locus tag^?: PNEUPAN003348000
• symbol: galR
• pan gene symbol^?: galR
• synonym:
• product: DNA-binding transcriptional regulator GalR

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: MDRSPN_RS08390 [old locus tag: MDRSPN_01599 ]
• symbol: galR
• product: DNA-binding transcriptional regulator GalR
• replicon: chromosome
• strand: +
• coordinates: 1611805..1612812
• length: 1008
• essential: unknown

⊟Accession numbers

• Location: NZ_AP018391 (1611805..1612812) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1635 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGGCTACCTTAAAAGACATTGCACAGCTAGCCTCTGTCTCTATCGCGACCGTATCCCGT
  GTCCTCAACCGCGACCAGAGCCTATCTGTTACAGAAGAAACCAGACACCGTATTTTAACC
  GTTGCTGAAGAGCTGGGCTACACCAAGCACCTCAAGACAGGCGAGTCCCATAAACCCAAG
  CAAAAGATTGCCATTATCCAATGGGTCAGCGAACAAGGGGAGCTAGACGACCTCTACTAC
  TACCAGATTCGCCTAGGAATAGAAAAAAGAGCCCAAGAGTTGGACTACGATATCTTGCGC
  TATTTTAATGACCACCCTTTTACCCTAAGCGAGGAAGTGATTGGGATTCTCTGCATCGGA
  AAGTTTAGTCGAGCTCAGATTTCTGCCTTTGAAGAATACCAAAAGCCTCTTGTATTTCTA
  GACAGCGATACACTTTCCCTGGGACATACCTGTATTATCACGGATTTTTACACTGCTATG
  AAACAGGTTGTCGATTATTTCCTCAGTCAAGGAATGGACCGTATCGGGATTCTAACAGGC
  CTTGAAGAAACAACTGACCAAGAAGAAATCATTGAGGATAAGCGGCTGGAAAATTTCAGA
  AACTACAGTCAAGCAAAAGGAATCTATCATGATGAACTGATCTTTCAAGGAAACTTTACT
  GCCCAGTCTGGCTATGACTTGATGAAGGAAGCCATTCAGAACTTGGGAGACCAACTCCCA
  CCAGCATTTTTCGCAGCCAGCGATAGTTTAGCCATCGGTGCCCTTCGTGCGCTCCAAGAA
  GCTGGAATCAGCCTACCAGACCGTGTCAGCCTCATTTCCTTTAACGACACCAGCCTGACC
  AAGCAGGTTTATCCTCCCCTCTCCAGCATCACAGTCTATACTGAAGAAATGGGGCGAGCA
  GGTATGGATATTCTTAACAAGGAAGTCCTCCACGGTCGGAAAATCCCTAGCCTGACCATG
  CTGGGAACCAGACTGACATTAAGAGAAAGTACCCTAAATCAAGAATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1008
  

⊟Protein

⊟General

• locus tag: MDRSPN_RS08390 [old locus tag: MDRSPN_01599 ]
• symbol: GalR
• description: DNA-binding transcriptional regulator GalR
• length: 335
• theoretical pI: 4.71616
• theoretical MW: 37878.7
• GRAVY: -0.238209

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 117.6)
  and 2 more
  Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 80.4)
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 72.4)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• PFAM:
  Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 104.9)
  and 4 more
  HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 60.2)
  Periplas_BP (CL0144) Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 35.4)
  Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 27.1)
  HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 14.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by GalR, TF important in Galactose utilization: in TIGR4

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.027804
  • TAT(Tat/SPI): 0.004294
  • LIPO(Sec/SPII): 0.004172
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000212512 NCBI
• UniProt:

⊟Protein sequence

• MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEELGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYFNDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAMKQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGNFTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFNDTSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQE

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_1635 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Muhammad Afzal, Sulman Shafeeq, Irfan Manzoor, Oscar P Kuipers
GalR Acts as a Transcriptional Activator of galKT in the Presence of Galactose in Streptococcus pneumoniae.
J Mol Microbiol Biotechnol: 2015, 25(6);363-71
[PubMed:26544195] [WorldCat.org] [DOI] (I p)