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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae 70585
  • locus tag: SP70585_0328 [new locus tag: SP70585_RS01620 ]
  • pan locus tag?: PNEUPAN001015000
  • symbol: SP70585_0328
  • pan gene symbol?: spuA
  • synonym:
  • product: pullulanase, extracellular

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP70585_0328 [new locus tag: SP70585_RS01620 ]
  • symbol: SP70585_0328
  • product: pullulanase, extracellular
  • replicon: chromosome
  • strand: -
  • coordinates: 283221..287063
  • length: 3843
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGAAAAACACCATCTCACACTGAGAAAAAAATGGTCTACAGCATCCGTTCCCTCAAA
    AATGGAACTGGTTCTGTCCTTATCGGCGCAAGCCTTGTCTTGCTTGCCATGGCTACACCA
    ACTATCTCATCCGACGAAAGTACACCAACCACTAACGAACCCAACAACAGAAATACAACC
    ACCCTTGCCCAACCTCTTACTGATACAGCAGCTGGCTCTGGTAAGAACGAAAGTGATATT
    TCTTCACCTAGAAATGCAAACGCTTCCCTAGAGAAAACAGAAGAAAAACCTGCTGCAAGC
    CCAGCCGATTCAGCACCACAAACTGGACAAGATCGTTCAAGTGAGCCAACTACTTCTACT
    AGTCCAGTAACAACTGAAACTAAGGCAGAAGAGCCCATCGAAGATAACTACTTCCGTATC
    CATGTCAAAAAACTTCCTGAAGAAAACAAGGATGCTCAAGGACTATGGACTTGGGACGAT
    GTTGAAAAACCATCTGAAAACTGGCCAAACGGAGCTTTGTCCTTCAAGGATGCCAAGAAA
    GATGACTACGGCTATTACCTAGATGTCAAATTAAAGGGAGAACAAGCCAAGAAAATTAGC
    TTCCTCATCAACAATACAGCTGGAAAAAATCTAACCGGCGATAAATCTGTAGAAAAACTA
    GTTCCAAAAATGAACGAAGCTTGGTTAGACCAAGATTACAAGGTTTTCTCTTACGAGCCA
    CAGCCTGCAGGAACTGTTCGCGTCAACTACTACCGCACAGATGGCAACTATGACAAGAAA
    TCTCTCTGGTACTGGGGAGATGTAAAAAATCCAAGTAGCGCTCAATGGCCTGACGGAACA
    GACTTTACGGCTACAGGCAAATATGGCCGCTATATCGACATTCCTCTTAATGAAGCCGCA
    AGAGAATTTGGATTTTTATTACTAGATGAGAGCAAACAAGGAGACGACGTGAAAATCCGT
    AAAGAAAATTATAAGTTCACAGATTTGAAAAATCATAGCCAAATTTTCCTAAAAGACGAT
    GATGAATCGATTTACACAAATCCATACTATGTCCATGATATCCGTATGACAGGAGCCCAA
    CACGTAGGCACTTCTAGCATTGAAAGTAGCTTTTCAACACTTGTCGGTGCTAAAAAAGAA
    GATATCCTCAAACACTCCAACATCACTAATCACCTAGGAAACAAGGTAACTATTACCGAT
    GTTGCAATCGATGAAGCTGGTAAGAAAGTGACCTACAGCGGAGATTTCTCTGACACAAAA
    CATCCTTATACTGTTAGCTACAATTCCGACCAATTCACTACCAAAACAAGCTGGCGCCTG
    AAAGATGAGACATACAGCTATGATGGCAAACTGGGAGCTGACCTAAAAGAAGAAGGAAAA
    CAAGTTGATTTGACCCTTTGGTCACCAAGTGCTGATAAGGTTTCTGTTGTTGTCTACGAC
    AAGAATGACCCTGACAAAGTAGTTGGAACTGTCGCTCTCGAAAAAGGGGAAAGAGGAACT
    TGGAAACAAACTCTAGACAGCACAAACAAACTCGGAATCACAGATTTCACTGGCTACTAT
    TATCAATACCAAATCGAGCGTCAAGGTAAAACTGTTCTTGCACTCGATCCTTACGCTAAA
    TCTCTTGCTGCTTGGAATAGCGACGATGCCAAGATTGACGATGCCCATAAAGTGGCTAAA
    GCCGCCTTTGTAGATCCAGCTAAACTCGGACCTCAAGACTTGACTTATGGTAAGATTCAC
    AATTTCAAGACTCGTGAAGACGCCGTTATCTACGAAGCTCATGTGCGTGACTTCACTTCA
    GATCCTGCCATTGCAAAAGACTTGACCAAACCATTTGGGACTTTTGAAGCCTTCATTGAA
    AAACTAGACTATCTCAAAGACTTGGGTGTAACCCATATCCAGCTCCTTCCAGTCTTGTCT
    TACTACTTTGTCAATGAATTGAAAAACCATGAACGCTTGTCTGACTATGCTTCAAGCAAC
    AGCAACTACAACTGGGGATATGACCCTCAAAACTACTTCTCCTTGACTGGTATGTACTCA
    AGCGATCCTAAGAATCCAGAAAAACGAATCGCAGAATTTAAAAACCTCATCAACGAAATC
    CACAAACGTGGTATGGGAGCTATCCTAGATGTCGTTTATAACCACACAGCCAAAGTCGAT
    ATCTTTGAAGACCTAGAGCCAAACTACTACCACTTTATGGATGCCGATGGCACACCTCGA
    ACTAGCTTTGGTGGTGGACGCTTGGGGACAACCCACCATATGACCAAACGGCTCCTAGTT
    GACTCTATCAAATACCTAGTTGATACCTACAAAGTGGATGGCTTCCGCTTCGATATGATG
    GGAGACCATGACGCCGCTTCTATCGAAGAAGCTTACAAGGCTGCACGCGCCCTCAATCCA
    AATCTAATCATGTTAGGCGAAGGTTGGAGAACCTATGCCGGTGATGAAAACATGCCTACT
    AAAGCTGCTGACCAAGATTGGATGAAACATACCGATACTGTCGCTGTCTTTTCAGATGAC
    ATCCGTAACAACCTCAAATCTGGTTATCCAAACGAAGGTCAACCTGCCTTTATCACAGGT
    GGCAAGCGTGATGTCAACACCATCTTTAAAAATCTCATTGCTCAACCAACTAACTTTGAA
    GCTGACAGCCCTGGAGATGTCATCCAATACATCGCAGCCCATGATAACTTGACCCTCTTT
    GACATCATTGCCCAGTCTATCAAAAAAGACCCAAGCAAGGCTGAGAACTATGCTGAAATC
    CACCGTCGTTTACGACTTGGAAATCTCATGGTCTTGACAGCTCAAGGAACTCCATTTATC
    CACTCCGGTCAGGAATATGGACGTACTAAACAATTCCGTGACCCAGCCTACAAGACTCCA
    GTAGCAGAGGATAAGGTTCCAAACAAATCTCACTTGTTGCGTGATAAGGACGGCAACCCA
    TTTGACTATCCTTACTTCATCCATGACTCTTACGATTCTAGTGATGCAGTCAACAAGTTT
    GACTGGACTAAGGCTACAGATGGTAAAGCTTATCCTGAAAATGTCAAGAGCCGTGACTAT
    ATGAAAGGTTTGATTGCCCTTCGTCAATCTACAGATGCCTTCCGACTTAAGAGTCTTCAA
    GATATCAAAGACCGTGTCCACCTCATCACTGTCCCAGGCCAAAATGGTGTGGAAAAAGAG
    GATGTAGTGATTGGCTATCAAATCACTGCTCCAAACGGCGATATCTACGCAGTCTTTGTC
    AATGCGGATGAAAAAGCTCGCGAATTTAATTTGGGAACTGCCTTTGCACATCTAAGAAAT
    GCGGAAGTTTTGGCAGATGAAAACCAAGCAGGATCAGTCGGAATTGCCAACCCGAAAGGA
    CTTGAATGGACTGAAAAAGGCTTGAAATTGAATGCCCTTACAGCTACTGTTCTTCGAGTC
    TCTCAAAATGGAACTAGCCATGAGTCAACTGCAGAAGAGAAACCAGACTCAACCCCTTCC
    AAGCCTGAACATCAAAATGAAGCTTCTCACCCTGCACATCAAGACCCAGCTCCAGAAGCT
    AGACCTGATTCTACTAAACCAGATGCCAAAGTAGCTGATGCGGAAAATAAACCTAGCCAA
    GCTACAGCTGATTCACAAGCTGAACAACCAGCACAAGAAGCACAAGCATCATCTGTAAAA
    GAAGCGGTTCGAAAAGAATCGGTAGAAAACTCTAGCAAGGAAAATATATCTGCAACCCCA
    GATAGACAAGCTGAACTTCCAAATACAGGAATCAAAAACGAAAACAAACTCCTATTTGCA
    GGAATCAGCCTCCTTGCGCTCCTTGGTCTCGGTTTCTTACTAAAAAATAAAAAAGAGAAC
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3843

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP70585_0328 [new locus tag: SP70585_RS01620 ]
  • symbol: SP70585_0328
  • description: pullulanase, extracellular
  • length: 1280
  • theoretical pI: 5.49731
  • theoretical MW: 142699
  • GRAVY: -0.732344

⊟Function[edit | edit source]

  • TIGRFAM:
    pullulanase, extracellular (TIGR02102; HMM-score: 2015.1)
    and 10 more
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 409.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides alpha-1,6-glucosidases, pullulanase-type (TIGR02103; EC 3.2.1.41; HMM-score: 251.3)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 151.4)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 95)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 33.9)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 33.8)
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 31.8)
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 25.5)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 23.8)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 17.5)
  • TheSEED  :
    • Pullulanase (EC 3.2.1.41)
    Carbohydrates Di- and oligosaccharides Maltose and Maltodextrin Utilization  Pullulanase (EC 3.2.1.41)
  • PFAM:
    GHD (CL0369) SpuA_C; SpuA C-terminal (PF18033; HMM-score: 116.1)
    Transthyretin (CL0287) PUD; Bacterial pullanase-associated domain (PF03714; HMM-score: 101.6)
    and 8 more
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 75.4)
    GHD (CL0369) CBM_48; Carbohydrate-binding module 48 (Isoamylase N-terminal domain) (PF02922; HMM-score: 44.3)
    no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 36)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 26.6)
    GHD (CL0369) Pullul_strch_C; Alpha-1,6-glucosidases, pullulanase-type, C-terminal (PF11852; HMM-score: 19.2)
    E-set (CL0159) FlgD_ig; FlgD Ig-like domain (PF13860; HMM-score: 14.5)
    no clan defined DUF3848; Protein of unknown function (DUF3848) (PF12959; HMM-score: 12.4)
    Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region (PF02932; HMM-score: 6.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.6
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 9.38
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • SignalP: Signal peptide SP(Sec/SPI) length 44 aa
    • SP(Sec/SPI): 0.848694
    • TAT(Tat/SPI): 0.081099
    • LIPO(Sec/SPII): 0.031837
    • Cleavage Site: CS pos: 44-45. ISS-DE. Pr: 0.5835
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISSDESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPAASPADSAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVRKESVENSSKENISATPDRQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNKKEN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: MalR* (repression) regulon, CcpA regulon
    MalR*(TF)important in Maltose utilization, Maltodextrin utilization; RegPrecise 
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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