TIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 31-JAN-2014

⊟Summary

• organism: Streptococcus pneumoniae 70585
• locus tag: SP70585_1322
• pan locus tag^?:
• symbol: SP70585_1322
• pan gene symbol^?: —
• synonym:
• product: 2-isopropylmalate synthase (Alpha-isopropylmalatesynthase) (Alpha-IPM synthetase)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SP70585_1322
• symbol: SP70585_1322
• product: 2-isopropylmalate synthase (Alpha-isopropylmalatesynthase) (Alpha-IPM synthetase)
• replicon: chromosome
• strand: -
• coordinates: 1226090..1226722
• length: 633
• essential: unknown

⊟Accession numbers

• Location: CP000918 (1226090..1226722) NCBI
• BioCyc:
• MicrobesOnline: 7477252 MicrobesOnline
• PneumoBrowse for strain D39V:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGAACTTTCAATTAGCTGAATATTCTTTACTGAAGAAATTTTCAGAAAATATAGGATTT
  ACCACACCAGAGGAATGTGGTGCCATCTTCAAATACCTGATTGAGAATGTCAAGACGGAT
  CGTCAGATCATCTATTCGCCTCACTGTCATGATGACCTCGGAATGGCAGTGGCAAATAGC
  CTTGCTGCTGTCAAGAATGGTGCAGGACGTGTTGAAGGGACTATCAATGGTATTAGGGAG
  CGAGCTGAAAATGCTGCTTTGGAAGAAATTGCAGTGGCTCTCAATATTCGCCAAGATTAC
  TACCAAGTAGAAACCAGTATTGTCCTAAATGAGACCATCAATACGTCAGAAATGGTTTCT
  CGCTTCTCTGGTATTCCAGTTCCTAAAAACAAAGCCGTCGTTGGTGGCAATACCTTCTCC
  CACGAATCTGGTATTCACCAAGATGGAGTCCTTAAAAATCCTCTCACTTATGAGATCATC
  ACACCTGAATTGGTTGGTGTTAAGATTCCGCTTGGAAAATTATCTGGTCGCCATGCTTTT
  GTTGAGAAACTGAGAGAATTGGCCCTAGATTTTACAGAAGAGGATATCAAACCACTCTTT
  GCTAAGTTCAAGGCACTGGCCGATAAGAAGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  633
  

⊟Protein

⊟General

• locus tag: SP70585_1322
• symbol: SP70585_1322
• description: 2-isopropylmalate synthase (Alpha-isopropylmalatesynthase) (Alpha-IPM synthetase)
• length: 210
• theoretical pI: 6.03662
• theoretical MW: 23224.4
• GRAVY: -0.17

⊟Function

• reaction:
  EC 2.3.3.13?  ExPASy
  2-isopropylmalate synthase Acetyl-CoA + 3-methyl-2-oxobutanoate + H₂O = (2S)-2-isopropylmalate + CoA
• TIGRFAM:
  Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00973; EC 2.3.3.13; HMM-score: 260.1)
  and 10 more
  isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 192.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 167.1)
  Metabolism Central intermediary metabolism Nitrogen fixation homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 167.1)
  homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 137.3)
  Unknown function General citramalate synthase (TIGR00977; EC 2.3.1.182; HMM-score: 119.8)
  Metabolism Amino acid biosynthesis Pyruvate family 2-isopropylmalate synthase (TIGR00970; EC 2.3.3.13; HMM-score: 46.6)
  Metabolism Energy metabolism Other 4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 38.8)
  Metabolism Transport and binding proteins Cations and iron carrying compounds oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 13.7)
  Metabolism Energy metabolism Other oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 13.7)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 11.8)
• TheSEED  :
  • 2-isopropylmalate synthase (EC 2.3.3.13)
  Amino Acids and Derivatives Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis  2-isopropylmalate synthase (EC 2.3.3.13)
  and 1 more
  Amino Acids and Derivatives Branched-chain amino acids Leucine Biosynthesis  2-isopropylmalate synthase (EC 2.3.3.13)
• PFAM:
  TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 100.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9521
  • Cytoplasmic Membrane Score: 0.0045
  • Cell wall & surface Score: 0.0008
  • Extracellular Score: 0.0426
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004777
  • TAT(Tat/SPI): 0.000269
  • LIPO(Sec/SPII): 0.000926
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: ACO17281 NCBI
• UniProt: C1C7N7 UniProt

⊟Protein sequence

• MNFQLAEYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQIIYSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQVETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEIITPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SP70585_1318 < SP70585_1319 < leuB < SP70585_1321 < SP70585_1322 < SP70585_1323 < SP70585_1324 < SP70585_1325

⊟Regulation

• regulator:

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V:

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications