⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae 70585
- locus tag: SP70585_1691 [new locus tag: SP70585_RS08380 ]
- pan locus tag?: PNEUPAN003047000
- symbol: SP70585_1691
- pan gene symbol?: psaA
- synonym:
- product: manganese ABC transporter substrate-binding lipoprotein (Adhesin B) (Saliva-binding protein)
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SP70585_1691 [new locus tag: SP70585_RS08380 ]
- symbol: SP70585_1691
- product: manganese ABC transporter substrate-binding lipoprotein (Adhesin B) (Saliva-binding protein)
- replicon: chromosome
- strand: +
- coordinates: 1559796..1560725
- length: 930
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: CP000918 (1559796..1560725) NCBI
- BioCyc: see SP70585_RS08380
- MicrobesOnline: 7477613 MicrobesOnline
- PneumoBrowse for strain D39V: SPV_1463 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901ATGAAAAAATTAGGTACATTACTCGTTCTCTTTCTTTCTGCAATCATTCTTGTAGCATGT
GCTAGCGGAAAAAAAGATACAACTTCTGGTCAAAAACTAAAAGTTGTTGCTACAAACTCA
ATCATCGCTGATATTACTAAAAATATTGCTGGTGACAAAATTGACCTTCATAGTATCGTT
CCGATTGGGCAAGACCCACACGAATACGAACCACTTCCTGAAGACGTTAAGAAAACTTCT
GAGGCTGATTTGATTTTCTATAACGGTATCAACCTTGAAACAGGTGGCAATGCTTGGTTT
ACAAAATTGGTAGAAAATGCCAAGAAAACTGAAAACAAAGACTACTTCGCAGTCAGCGAC
GGCGTTGATGTTATCTACCTCGAAGGTCAAAATGAAAAAGGAAAAGAAGACCCACACGCT
TGGCTTAACCTTGAAAACGGTATTATTTTTGCTAAAAATATCGCCAAACAATTGAGCGCC
AAAGACCCTAACAATAAAGAATTCTATGAAAAAAATCTCAAAGAATATACTGATAAGTTA
GACAAACTTGATAAAGAAAGTAAGGATAAATTTAATAAAATCCCTGCTGAAAAGAAACTC
ATTGTAACCAGCGAAGGAGCATTCAAATACTTCTCTAAAGCCTATGGTGTCCCAAGTGCC
TACATCTGGGAAATCAATACTGAAGAAGAAGGAACTCCTGAACAAATCAAGACCTTGGTT
GAAAAACTTCGCCAAACAAAAGTTCCATCACTCTTTGTAGAATCAAGTGTGGATGACCGT
CCAATGAAAACTGTTTCTCAAGACACAAACATCCCAATCTACGCACAAATCTTTACTGAC
TCTATCGCAGAACAAGGTAAAGAAGGCGACAGCTACTACAGCATGATGAAATACAACCTT
GACAAGATTGCTGAAGGATTGGCAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SP70585_1691 [new locus tag: SP70585_RS08380 ]
- symbol: SP70585_1691
- description: manganese ABC transporter substrate-binding lipoprotein (Adhesin B) (Saliva-binding protein)
- length: 309
- theoretical pI: 5.0621
- theoretical MW: 34594.1
- GRAVY: -0.501618
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Unknown substrate anchored repeat ABC transporter, substrate-binding protein (TIGR03772; HMM-score: 143.7)and 1 moreAmino acid biosynthesis Serine family phosphoserine transaminase (TIGR01364; EC 2.6.1.52; HMM-score: 12.1)
- TheSEED :
- Manganese/zinc ABC transporter (EC 7.2.2.5), substrate-binding protein ScaA
- PFAM: Chelatase (CL0043) ZnuA; Zinc-uptake complex component A periplasmic (PF01297; HMM-score: 262.8)and 2 moreHybrid (CL0105) DUF2118; Uncharacterized protein conserved in archaea (DUF2118) (PF09891; HMM-score: 14.8)no clan defined OST_P2; Oligosaccharyltransferase Peripheral 2 domain (PF18235; HMM-score: 12.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.01
- Cytoplasmic Membrane Score: 9.99
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helix: 1
- SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
- SP(Sec/SPI): 0.001806
- TAT(Tat/SPI): 0.000062
- LIPO(Sec/SPII): 0.997937
- Cleavage Site: CS pos: 19-20. LVA-CA. Pr: 0.9977
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIADITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTKLVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSAKDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVPSAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQIFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: MntR* (repression) regulon
MntR* (TF) important in Manganese homeostasis; RegPrecise transcription unit transferred from TIGR4 data RegPrecise
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_1463 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
D E Briles, E Ades, J C Paton, J S Sampson, G M Carlone, R C Huebner, A Virolainen, E Swiatlo, S K Hollingshead
Intranasal immunization of mice with a mixture of the pneumococcal proteins PsaA and PspA is highly protective against nasopharyngeal carriage of Streptococcus pneumoniae.
Infect Immun: 2000, 68(2);796-800
[PubMed:10639448] [WorldCat.org] [DOI] (P p)Hsing-Ju Tseng, Alastair G McEwan, James C Paton, Michael P Jennings
Virulence of Streptococcus pneumoniae: PsaA mutants are hypersensitive to oxidative stress.
Infect Immun: 2002, 70(3);1635-9
[PubMed:11854257] [WorldCat.org] [DOI] (P p)Sandra Romero-Steiner, Tamar Pilishvili, Jacquelyn S Sampson, Scott E Johnson, Annie Stinson, George M Carlone, Edwin W Ades
Inhibition of pneumococcal adherence to human nasopharyngeal epithelial cells by anti-PsaA antibodies.
Clin Diagn Lab Immunol: 2003, 10(2);246-51
[PubMed:12626450] [WorldCat.org] [DOI] (P p)J McCluskey, J Hinds, S Husain, A Witney, T J Mitchell
A two-component system that controls the expression of pneumococcal surface antigen A (PsaA) and regulates virulence and resistance to oxidative stress in Streptococcus pneumoniae.
Mol Microbiol: 2004, 51(6);1661-75
[PubMed:15009893] [WorldCat.org] [DOI] (P p)Jason W Johnston, Lisa E Myers, Martina M Ochs, William H Benjamin, David E Briles, Susan K Hollingshead
Lipoprotein PsaA in virulence of Streptococcus pneumoniae: surface accessibility and role in protection from superoxide.
Infect Immun: 2004, 72(10);5858-67
[PubMed:15385487] [WorldCat.org] [DOI] (P p)Julie M Anderton, Gowrisankar Rajam, Sandra Romero-Steiner, Susan Summer, Andrew P Kowalczyk, George M Carlone, Jacquelyn S Sampson, Edwin W Ades
E-cadherin is a receptor for the common protein pneumococcal surface adhesin A (PsaA) of Streptococcus pneumoniae.
Microb Pathog: 2006, 42(5-6);225-36
[PubMed:17412553] [WorldCat.org] [DOI] (P p)A M Berry, J C Paton
Sequence heterogeneity of PsaA, a 37-kilodalton putative adhesin essential for virulence of Streptococcus pneumoniae.
Infect Immun: 1996, 64(12);5255-62
[PubMed:8945574] [WorldCat.org] [DOI] (P p)J A Tharpe, H Russell
Purification and seroreactivity of pneumococcal surface adhesin A (PsaA).
Clin Diagn Lab Immunol: 1996, 3(2);227-9
[PubMed:8991641] [WorldCat.org] [DOI] (P p)A Dintilhac, G Alloing, C Granadel, J P Claverys
Competence and virulence of Streptococcus pneumoniae: Adc and PsaA mutants exhibit a requirement for Zn and Mn resulting from inactivation of putative ABC metal permeases.
Mol Microbiol: 1997, 25(4);727-39
[PubMed:9379902] [WorldCat.org] [DOI] (P p)