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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 22-FEB-2023

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_RS05825 [old locus tag: SPAP_1177 ]
  • pan locus tag?: PNEUPAN002044000
  • symbol: prmC
  • pan gene symbol?: hemK
  • synonym:
  • product: peptide chain release factor N(5)-glutamine methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_RS05825 [old locus tag: SPAP_1177 ]
  • symbol: prmC
  • product: peptide chain release factor N(5)-glutamine methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1115434..1116273
  • length: 840
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_014494 (1115434..1116273) NCBI
  • BioCyc: SPAP_RS05825 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0907 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAATTAGCTCAATTATTTTCAAATTTTGAAGAAGAGTTGATAAGACAAGGAGAGGAA
    GCGGAAAGCCTCTCTTTTGTCTATCGTAGTCTGAAAAATCTATCTTTTACAGACTTTATT
    TTTGCCCTCCAGCAGGAGGTGACAACAGAGGAAGAAAAACAATTTGTAGAAGATATTTAC
    CAGCAACTAGCAGCTCACAAGCCAGCTCAGTACATCATTGGACAGGCAGATTTTTATGGA
    ATGCATTTAAAGGTGGATGAACGGGTTTTGATTCCTCGTCCAGAAACAGAGGAGTTAGTG
    GAGCTTATTCTAACTGAAAATCTTGAGACGAATCTCTCAGTTCTGGATATTGGAACAGGT
    AGTGGAGCTATTGCTCTCGCTCTAGCAAAAAATAGACCAGATTGGTCAGTGACAGCAGCA
    GATGTTTCTCAAGAGGCCTTAGAGCTTGCATCAGAGAATGCTAGCGATCAAAATCTTAAT
    ATATTTTTTAAAAAATCTGATTGTTTTGCAGAAATTTCTGAAAAATATGATATAATTGTA
    TCCAATCCACCCTATATCTCTCGTGAAGATGAGTCAGAGGTAGGCTTGAATGTTTTGCAT
    TCGGAGCCTCATCTAGCTCTCTTTGCAGACGAGGATGGCCTAGCTATTTACTGTAGAATT
    GCGGAAGATGCAAAAGACTATCTCAAGGATGGTGGTAAGATTTACCTTGAAATTGGATAC
    AAGCAAGGTCAAAGTGTTCCCGAACTTTTTAGAAAACATCTTCCTGAAAAAAAAGTTCGA
    ACACTCAAAGACCAATTTGGTCAAAATAGGATGGTTGTGGTTGATGATGGACAGGATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_RS05825 [old locus tag: SPAP_1177 ]
  • symbol: PrmC
  • description: peptide chain release factor N(5)-glutamine methyltransferase
  • length: 279
  • theoretical pI: 4.23562
  • theoretical MW: 31605.2
  • GRAVY: -0.353763

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.297?  ExPASy
    Peptide chain release factor N5-glutamine methyltransferase S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N5-methyl-L-glutamine
  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 274.7)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 229.9)
    and 23 more
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 177.8)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 101.9)
    Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 66)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 34)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 33.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 33.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 33.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 30.6)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 22.9)
    Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 21.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 20.2)
    Cellular processes Cellular processes Toxin production and resistance tellurite resistance protein TehB (TIGR00477; HMM-score: 19.6)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 19)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 16.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll C-20 methyltransferase BchU (TIGR02716; EC 2.1.1.-; HMM-score: 16.3)
    Genetic information processing Transcription RNA processing 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 15.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 15.5)
    methyltransferase, Rta_06860 family (TIGR04290; EC 2.1.1.-; HMM-score: 15.2)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 15.2)
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG (TIGR00138; EC 2.1.1.170; HMM-score: 14.7)
    methyltransferase, FkbM family (TIGR01444; HMM-score: 14.5)
    2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 14.1)
    Genetic information processing Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 12.5)
  • TheSEED: see SPAP_1177
  • PFAM:
    NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 76.9)
    and 27 more
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 53.8)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 47.8)
    Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 35.6)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 33.2)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 30.7)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 30.3)
    Methyltransf_10; RNA methyltransferase (PF05971; HMM-score: 27.8)
    Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 24.4)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 24.3)
    Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 23.5)
    no clan defined PrmC_N; PrmC N-terminal domain (PF17827; HMM-score: 21.9)
    NADP_Rossmann (CL0063) Methyltransf_16; Lysine methyltransferase (PF10294; HMM-score: 20.2)
    TehB; Tellurite resistance protein TehB (PF03848; HMM-score: 18.4)
    PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 17.6)
    Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 16.9)
    TrmK; tRNA (adenine(22)-N(1))-methyltransferase (PF04816; HMM-score: 16.9)
    NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 15.9)
    Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 15.5)
    N6_N4_Mtase; DNA methylase (PF01555; HMM-score: 15.4)
    Methyltransf_2; O-methyltransferase domain (PF00891; HMM-score: 14.7)
    CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 14.2)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 13.8)
    Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 13.5)
    GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 13)
    DUF938; Protein of unknown function (DUF938) (PF06080; HMM-score: 12.7)
    HTH (CL0123) Sigma70_ECF; ECF sigma factor (PF07638; HMM-score: 12.5)
    NADP_Rossmann (CL0063) RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9952
    • Cytoplasmic Membrane Score: 0.001
    • Cell wall & surface Score: 0.0009
    • Extracellular Score: 0.0029
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002524
    • TAT(Tat/SPI): 0.000473
    • LIPO(Sec/SPII): 0.000308
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000761903 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLNIFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYCRIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDDGQD

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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