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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 22-FEB-2023

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_RS05900 [old locus tag: SPAP_1193 ]
  • pan locus tag?: PNEUPAN002029000
  • symbol: pitB
  • pan gene symbol?: pitB
  • synonym:
  • product: PI-2 pilus major pilin PitB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_RS05900 [old locus tag: SPAP_1193 ]
  • symbol: pitB
  • product: PI-2 pilus major pilin PitB
  • replicon: chromosome
  • strand: -
  • coordinates: 1127779..1128975
  • length: 1197
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_014494 (1127779..1128975) NCBI
  • BioCyc: SPAP_RS05900 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAAAAAACATTCTTTAAAAAGCTATTCACTGCAAGCATTGCAGCTATAACCGCTTTG
    TCCGTATTCAGAGGTGTCCCGACTTTTGCGGATGATAATTCAGCAATAACCAAAGCAAAT
    GGTGAAAATAATGCTGTTGTGAAGATTAATAAAACGTTGAATATTGCAGAGGGAATAACA
    ACACCAACAGCGACATTTACATTTAAGTTTACAGAAAAAACAGGACAATCTTCTAACGGT
    GCGCCATATCAAACCGGAGTTGCAATTCCAGATAGAAATGTAGAATACAATAAAAATGAT
    CACCCAACTGCTGATAAGATTCAAAAAGCAACAGAAGACATTTTTTCGGGAGTTGCTTAT
    GGCCATGCTGGTGAATACGTTTATGATGTAGCGGAAGCAAAAACTGGATGGCAGGCGATT
    ACCAAAAATGGTAAAACAATTGATGCCATGAGATACGACAAACGTACATATGAAATGCAC
    GTTATTGTTAAGAATAAAGTAAATGGTGGTGTCTATATTTCATCAGTATACTTTAAGGAA
    AATAATAAATCTAACGCCCCTAAAGTAGAACCAAGTGAACAAGGCGTTTATAATTTATTT
    GATAACACATATACCAAAGACGCAAGTAAGGAGCCTAATCCTGATGATCCGAGTCAAGTA
    GACCCCAATGCGAAAGCATTAACAATTACTAAAAAAGTTGATGGAGCTTCAGGGGATAAA
    ACAAGAGATTTCCAATTCCATATCAAGATTCAACTTCCAAGTACAAATAAAACAGCAGAA
    ACCCCTGTTACGAATATTATAGTAAAACATGGATCTAAGTCAGAGGTGTTGGCAGTAGTG
    ACCCCAGCAGATACAGTTGAGTACAATTTTACTCTTAAAGATGGTGAAACATTTACAGTT
    GAACAACTACCAGCAGGTTCTAAATATACAGTAACTGAAACTGGAGTAGCAGGTTATACA
    GATTCATCAATTTATACTACAAATGGTGCAGAACAAACATCTCAAGGACAAAAAAATGTA
    GATTTTACATTAACAGATATCCTCATAGGTGAAAAGAAAAACGACAACAAAGTTACTAAC
    AAAATCGACGACGTTACTCCTACTGGTCTCTTGATTGATAACCTTCCATTCATTTTGATG
    ATTGGTCTTGGTTTGGCTGGATTTGTTGTCTTGTCTAAAAAACGTAGAGAAGCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1197

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_RS05900 [old locus tag: SPAP_1193 ]
  • symbol: PitB
  • description: PI-2 pilus major pilin PitB
  • length: 398
  • theoretical pI: 7.79482
  • theoretical MW: 43393.4
  • GRAVY: -0.468844

⊟Function[edit | edit source]

  • TIGRFAM:
    streptococcal pilin isopeptide linkage domain (TIGR03786; HMM-score: 13.8)
    and 1 more
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 7.7)
  • TheSEED: see SPAP_1193
  • PFAM:
    Transthyretin (CL0287) FctA; Spy0128-like isopeptide containing domain (PF12892; HMM-score: 40.7)
    DUF5979; Domain of unknown function (DUF5979) (PF19407; HMM-score: 36.8)
    and 2 more
    no clan defined DUF6406; Family of unknown function (DUF6406) (PF19944; HMM-score: 15.2)
    Transthyretin (CL0287) SpaA_2; Prealbumin-like fold domain (PF19403; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 2
  • DeepLocPro: Cell wall & surface
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.236
    • Cell wall & surface Score: 0.5252
    • Extracellular Score: 0.2387
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.990962
    • TAT(Tat/SPI): 0.005911
    • LIPO(Sec/SPII): 0.001191
    • Cleavage Site: CS pos: 30-31. TFA-DD. Pr: 0.9737
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000750610 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENNAVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHPTADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMHVIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPSQVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEVLAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTSQGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLSKKRREA

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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