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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 07-FEB-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_RS07370 [old locus tag: SPAP_1497 ]
  • pan locus tag?: PNEUPAN002795000
  • symbol: SPAP_RS07370
  • pan gene symbol?: nox
  • synonym:
  • product: FAD-dependent oxidoreductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_RS07370 [old locus tag: SPAP_1497 ]
  • symbol: SPAP_RS07370
  • product: FAD-dependent oxidoreductase
  • replicon: chromosome
  • strand: -
  • coordinates: 1406406..1407785
  • length: 1380
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_014494 (1406406..1407785) NCBI
  • BioCyc: SPAP_RS07370 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1298 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGTAAAATCGTTGTAGTCGGTGCTAACCACGCTGGTACAGCATGTATCAATACCATG
    TTGGATAATTTTGGAAATGAGAACGAAATTGTTGTATTTGACCAAAACTCTAACATCTCT
    TTCCTAGGATGTGGAATGGCTCTTTGGATTGGTGAACAAATTGACGGTGCTGAAGGCTTG
    TTCTATTCTGATAAAGAAAAATTGGAAGCTAAAGGTGCTAAAGTTTACATGAACTCACCT
    GTTCTTTCAATCGACTATGATAACAAAGTAGTTACAGCGGAAGTTGAAGGAAAAGAGCAC
    AAAGAATCATACGAAAAATTGATTTTCGCTACAGGTTCTACACCAATCTTGCCACCAATC
    GAAGGTGTTGAAATTGTTAAAGGAAACCGCGAATTTAAAGCAACTCTTGAAAACGTACAA
    TTCGTGAAATTGTACCAAAATGCTGAAGAAGTTATCAATAAACTTTCTGACAAGAGCCAA
    CACCTCGACCGTATCGCCGTTGTTGGTGGTGGTTACATCGGTGTTGAACTTGCTGAAGCC
    TTTGAACGTCTTGGAAAAGAAGTTGTCCTTGTTGATATCGTTGATACTGTCTTGAACGGT
    TACTATGACAAAGACTTCACACAAATGATGGCGAAGAACTTGGAAGATCACAACATCCGC
    TTGGCTCTAGGTCAAACTGTTAAAGCAATCGAAGGTGACGGTAAAGTTGAACGCTTGATT
    ACTGACAAAGAAAGCTTTGACGTGGATATGGTTATCCTTGCAGTTGGTTTCCGTCCAAAC
    ACAGCCCTTGCAGGTGGTAAGATCGAACTCTTCCGCAACGGTGCCTTCCTTGTAGACAAG
    AAACAAGAAACATCTATCCCAGACGTTTACGCTGTTGGTGACTGTGCGACTGTTTATGAC
    AATGCTCGTAAAGATACAAGCTATATCGCTCTTGCTTCAAATGCTGTTCGCACTGGTATC
    GTTGGTGCCTACAATGCTTGTGGACATGAATTGGAAGGAATCGGTGTTCAAGGTTCAAAT
    GGTATCTCAATCTACGGTCTTCACATGGTTTCAACTGGTTTGACTCTTGAAAAAGCGAAA
    GCTGCTGGTTACAACGCAACTGAAACAGGCTTTAACGATCTTCAAAAACCAGAATTCATG
    AAACATGACAACCATGAAGTAGCAATTAAGATTGTCTTTGACAAAGATAGCCGTGAAATT
    CTTGGTGCCCAAATGGTTTCACATGATATTGCAATTAGCATGGGAATCCACATGTTCTCA
    CTTGCTATCCAAGAGCATGTGACAATTGATAAATTGGCATTGACAGACCTCTTCTTCTTG
    CCACACTTCAACAAACCATACAACTACATCACAATGGCTGCCCTTACGGCTGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_RS07370 [old locus tag: SPAP_1497 ]
  • symbol: SPAP_RS07370
  • description: FAD-dependent oxidoreductase
  • length: 459
  • theoretical pI: 4.75146
  • theoretical MW: 50254.9
  • GRAVY: -0.0664488

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 286.6)
    and 32 more
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 142.6)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 133.2)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 129.4)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 90.2)
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 64.5)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 62)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 59.6)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 57.6)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 48.6)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 48.6)
    trypanothione-disulfide reductase (TIGR01423; EC 1.8.1.12; HMM-score: 44.9)
    thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 40.6)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 38.5)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 27.6)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 25.6)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 25.3)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 24.7)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 24.5)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 22.8)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 21.7)
    Genetic information processing Protein fate Protein folding and stabilization xanthine dehydrogenase accessory protein XdhC (TIGR02964; HMM-score: 20.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other xanthine dehydrogenase accessory protein XdhC (TIGR02964; HMM-score: 20.2)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 19.4)
    oxidoreductase/SelD-related fusion protein (TIGR04369; HMM-score: 18.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 17.2)
    hydrogenase maturation protease (TIGR00072; HMM-score: 13.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 13.4)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 12.3)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, gamma subunit family (TIGR01375; EC 1.5.3.1; HMM-score: 11.9)
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnB (TIGR03944; HMM-score: 11.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 10.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 10.5)
  • TheSEED: see SPAP_1497
  • ⊞PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 227.2)
    and 19 more
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 76.9)
    Reductase_C (CL0608) Pyr_redox_dim; Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain (PF02852; HMM-score: 51.6)
    NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 43)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 36.6)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 24.7)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 23.3)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 23)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 22.7)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 22.5)
    Lys_Orn_oxgnase; L-lysine 6-monooxygenase/L-ornithine 5-monooxygenase (PF13434; HMM-score: 18.6)
    Amino_oxidase; Flavin containing amine oxidoreductase (PF01593; HMM-score: 17.3)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 16.4)
    Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 15.1)
    GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 14.7)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 14.1)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 12.3)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 12)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 12)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 10.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012214
    • TAT(Tat/SPI): 0.000256
    • LIPO(Sec/SPII): 0.04994
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000036793 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGCGMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKESYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQHLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHNIRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAFLVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGIGVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIVFDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYITMAALTAEK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

I Auzat, S Chapuy-Regaud, G Le Bras, D Dos Santos, A D Ogunniyi, I Le Thomas, J R Garel, J C Paton, M C Trombe
The NADH oxidase of Streptococcus pneumoniae: its involvement in competence and virulence.
Mol Microbiol: 1999, 34(5);1018-28
[PubMed:10594826] [WorldCat.org] [DOI] (P p)Jun Yu, Alexander P Bryant, Andrea Marra, Michael A Lonetto, Karen A Ingraham, Alison F Chalker, David J Holmes, David Holden, Martin Rosenberg, Damien McDevitt
Characterization of the Streptococcus pneumoniae NADH oxidase that is required for infection.
Microbiology (Reading): 2001, 147(Pt 2);431-438
[PubMed:11158360] [WorldCat.org] [DOI] (P p)

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