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TIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae CGSP14
  • locus tag: SPCG_1709
  • pan locus tag?:
  • symbol: SPCG_1709
  • pan gene symbol?: —
  • synonym:
  • product: primosome assembly protein PriA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPCG_1709
  • symbol: SPCG_1709
  • product: primosome assembly protein PriA
  • replicon: chromosome
  • strand: -
  • coordinates: 1682402..1683376
  • length: 975
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP001033 (1682402..1683376) NCBI
  • BioCyc:
  • MicrobesOnline: 5761044 MicrobesOnline
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGCTAGAGGCTATCCAAGACCGTCTGGTTAAAAAAGAGCAGATGGTTCTCATGCTCAAT
    CGCCGTGGTTATTCTAGCTTTGTCATGTGTCGGGAGTGTGGGACGGTGGATACTTGTCCC
    AACTGCGATATTTCTCTGACCTTGCATATGGATACCAAGACTATGAACTGCCATTATTGT
    GGTTTCTCGAAGGATATTCCTCAGGTCTGTCCTAACTGTAAGAGCCACAGTATTCGTTAC
    TATGGGACGGGAACTCAGAAGGCTTATGATGAGCTAGCAGAACTCTTTCCCCAAGCTCGC
    ATTTTGAGGATGGATGTGGATACGACTCGAAAGAAAGGCAGTCACCAAGCCTTGCTTGAC
    CAGTTTGGGCGAGGGGAAGCGGATATTTTACTTGGTACTCAGATGATTGCAAAGGGTTTG
    GATTTTCCCAATGTTACCCTAGTCGGAGTTCTAAATGCGGATACGGCCTTGAATCTGCCT
    GATTTCCGTTCTTCTGAGAGAACCTTCCAGCTCTTGACTCAGGTGGCAGGTCGAGCAGGT
    CGGGCTGAAAAAGCAGGTCAAGTCTTGATACAGTCCTACAATCCGCAGCACTATGCTATT
    CGATTTGCCAAGGATCAAGATTACGAAGGCTTTTATTCCTATGAAATGGGAATCAGACGA
    CAACTCGGCTATCCACCTTACTATTTCACGATTGGTATTACCCTTTCTCACAAGAAAGAA
    GAAGAGGTTTTCAAACGTGCCTATGAAGTCATGAACATTTTGCGGTCAGGCTTGTCAGAG
    ACCAGTCCCATTCTGGGGCCAACGCCAAAACCGATTGCCCGTACCCACAACCTCTATCAT
    TACCAGATTTTAATTAAATACCGTTTAGAAGATGAGCTGGGGCCGACCCTCAACCAGGTC
    CTGGCCTTGACTCAAGAACGGGAAAATAGTGAGCTCCGTCTCAGCATTGACCATGAGCCG
    CAGCAATTTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    975

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPCG_1709
  • symbol: SPCG_1709
  • description: primosome assembly protein PriA
  • length: 324
  • theoretical pI: 7.4941
  • theoretical MW: 37052.2
  • GRAVY: -0.420988

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 387.9)
    and 5 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 33.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 25.8)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 19.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 13.2)
    glycosyltransferase, TIGR04182 family (TIGR04182; HMM-score: 11.5)
  • TheSEED  :
    • Helicase PriA essential for oriC/DnaA-independent DNA replication
    DNA Metabolism DNA replication DNA-replication  Helicase PriA essential for oriC/DnaA-independent DNA replication
  • PFAM:
    no clan defined PriA_C; Primosomal protein N C-terminal domain (PF18074; HMM-score: 68)
    and 9 more
    Zn_Beta_Ribbon (CL0167) PriA_CRR; PriA DNA helicase Cys-rich region (CRR) domain (PF18319; HMM-score: 44)
    P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 38.9)
    no clan defined zf-CHY; CHY zinc finger (PF05495; HMM-score: 12.5)
    Zn_Beta_Ribbon (CL0167) zinc-ribbons_6; zinc-ribbons (PF07191; HMM-score: 11.8)
    Zn_Tnp_IS1595; Transposase zinc-ribbon domain (PF12760; HMM-score: 10.6)
    OrfB_Zn_ribbon; Putative transposase DNA-binding domain (PF07282; HMM-score: 10.3)
    DZR; Double zinc ribbon (PF12773; HMM-score: 9.8)
    no clan defined HypA; Hydrogenase/urease nickel incorporation, metallochaperone, hypA (PF01155; HMM-score: 9)
    Zn_Beta_Ribbon (CL0167) TF_Zn_Ribbon; TFIIB zinc-binding (PF08271; HMM-score: 7.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.219899
    • TAT(Tat/SPI): 0.030862
    • LIPO(Sec/SPII): 0.01343
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACB90961 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MLEAIQDRLVKKEQMVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDYEGFYSYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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