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serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 24-JAN-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae CGSP14
  • locus tag: SPCG_RS00310 [old locus tag: SPCG_0053 ]
  • pan locus tag?: PNEUPAN000697000
  • symbol: purD
  • pan gene symbol?: purD
  • synonym:
  • product: phosphoribosylamine--glycine ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPCG_RS00310 [old locus tag: SPCG_0053 ]
  • symbol: purD
  • product: phosphoribosylamine--glycine ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 56060..57322
  • length: 1263
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAACTGCTTGTTGTCGGTTCTGGTGGTCGTGAGCATGCGATTGCTAAAAAGTTACTT
    GAATCAAAAGACGTGGAAAAAGTCTTTGTAGCTCCTGGGAATGATGGGATGACTCTGGAT
    GGTTTGGAATTGGTAAATATCTCTATTTCCGAACATTCTAAATTGATTGACTTCGCAAAG
    ACCAATGATGTTGCTTGGACCTTTATCGGTCCAGATGATGCCCTTGCTGCTGGTATCGTG
    GATGATTTTAACCAAGCTGGACTTAAGGCCTTTGGTCCGACTAGGGCTGCAGCGGAGCTG
    GAGTGGTCCAAGGATTTCGCCAAGGAAATCATGGTCAAATACGGCGTTCCGACAGCAACA
    TATGGCACATTTTCAGATTTCGAGGAAGCCAAGGCCTATATCGAAAAGCATGGTGCGCCT
    ATCGTAGTCAAGGCGGATGGCTTGGCACTTGGGAAGGGTGTCGTCGTTGCTGATACGGTT
    GAGCAAGCGGTCGAAGCCGCTCATGAGATGCTTTTGGACAATAAATTTGGTGACTCAGGT
    GCGCGCGTGGTTATTGAGGAATTCCTTGAAGGAGAGGAATTTTCACTCTTTGCCTTTGTC
    AATGGTGATAAGTTCTACATCATGCCAACGGCTCAGGACCACAAACGTGCCTATGATGGC
    GACAAAGGGCCTAACACGGGTGGTATGGGTGCCTATGCGCCAGTCCCACACTTACCACAG
    AGTGTAGTTGATACAGCGGTTGACACCATTGTCAAGCCAGTTCTAGAAGGGGTGATTAAA
    GAAGGTCGCCCTTATCTGGGAGTTCTTTACGCAGGGCTTATCCTGACAGCTGATGGACCG
    AAAGTCATTGAGTTCAACGCTCGGTTCGGAGATCCAGAAACTCAGATTATCTTGCCTCGC
    TTGACCTCTGACTTTGCTCAAAATATCACAGATATCCTGGATAGCAAGGGGCCAAATATC
    ATGTGGACGGACAAGGGTGTGACTCTGGGTGTGGTTGTCGCATCCAAGGGCTACCCGCTA
    GACTATGAAAGGGGCGTTGAGTTGCCAGCCAAGACAGAAGGCGATGTCATCACCTACTAT
    GCAGGGGCTAAGTTTGCGGAAAATAGCAGAGCACTGCTCTCAAACGGCGGACGAGTTTAT
    ATGCTCGTTACCACAGCAGATACCGTCAAAGAAGCCCAAGCCAGCATATACCAAGAACTA
    TACCAACAAAAAATAGAAGGACTCTTCTACCGAACAGATATCGGAAGCAAGGCAATTAAG
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1263

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPCG_RS00310 [old locus tag: SPCG_0053 ]
  • symbol: PurD
  • description: phosphoribosylamine--glycine ligase
  • length: 420
  • theoretical pI: 4.57333
  • theoretical MW: 45332.2
  • GRAVY: -0.0688095

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.4.13?  ExPASy
    Phosphoribosylamine--glycine ligase ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylamine--glycine ligase (TIGR00877; EC 6.3.4.13; HMM-score: 497.4)
    and 12 more
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 32.5)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 30.4)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 28.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 25.2)
    Metabolism Energy metabolism TCA cycle succinate-CoA ligase, beta subunit (TIGR01016; EC 6.2.1.-; HMM-score: 23.5)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 20.9)
    alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 19.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 16)
    lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 15.9)
    GNAT-family acetyltransferase TIGR03103 (TIGR03103; HMM-score: 14.5)
    Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 13.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs glutamate--cysteine ligase/glutathione synthase (TIGR01435; EC 6.3.2.2,6.3.2.3; HMM-score: 11.9)
  • TheSEED: see SPCG_0053
  • PFAM:
    ATP-grasp (CL0179) GARS_A; Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (PF01071; HMM-score: 271.5)
    and 10 more
    PreATP-grasp (CL0483) GARS_N; Phosphoribosylglycinamide synthetase, N domain (PF02844; HMM-score: 112)
    Hybrid (CL0105) GARS_C; Phosphoribosylglycinamide synthetase, C domain (PF02843; HMM-score: 77.9)
    ATP-grasp (CL0179) ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 24.5)
    CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 24.3)
    ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 21.8)
    ATP-grasp_2; ATP-grasp domain (PF08442; HMM-score: 21.2)
    ATP-grasp_4; ATP-grasp domain (PF13535; HMM-score: 19.2)
    ATP-grasp_5; ATP-grasp domain (PF13549; HMM-score: 16.1)
    RimK; RimK-like ATP-grasp domain (PF08443; HMM-score: 14.7)
    ATPgrasp_ST; Sugar-transfer associated ATP-grasp (PF14397; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006676
    • TAT(Tat/SPI): 0.00019
    • LIPO(Sec/SPII): 0.00082
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLELVNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEWSKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVADTVEQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAYDGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILTADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKGPNIMWTDKGVTLGVVVASKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEAQASIYQELYQQKIEGLFYRTDIGSKAIK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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