PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 16-DEC-2024

⊟Summary

• organism: Streptococcus pneumoniae CGSP14
• locus tag: SPCG_RS03295 [old locus tag: SPCG_0628 ]
• pan locus tag^?: PNEUPAN001587000
• symbol: miaA
• pan gene symbol^?: miaA
• synonym:
• product: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPCG_RS03295 [old locus tag: SPCG_0628 ]
• symbol: miaA
• product: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA
• replicon: chromosome
• strand: +
• coordinates: 635295..636179
• length: 885
• essential: unknown

⊟Accession numbers

• Location: NC_010582 (635295..636179) NCBI
• BioCyc: SPCG_RS03295 BioCyc
• MicrobesOnline: see SPCG_0628
• PneumoBrowse for strain D39V: SPV_0583 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  
  ATGAAAACAAAAATAATTGTGATTGTTGGACCGACTGCTGTTGGAAAGACGGCTCTAGCT
  ATTGAAGTTGCAAAGCGTTTTAATGGCGAAGTGGTTAGTGGAGATAGCCAGCAAGTCTAT
  CGAGGACTTGATATTGGGACGGCCAAGGCTAGTCCAGAAGAGCAGGCAGCTGTTCCTCAT
  CATTTAATCGATGTTAGAGAGATAACCGAGTCTTACTCGGCTTTTGATTTTGTTTCAGAA
  GCTAAGATGACTATTGAGGGTATTCACAACCGTGGCAAGCTAGCCATTATCGCCGGTGGG
  ACTGGACTTTATATCCAGAGCTTGCTAGAAGGTTACCACCTAGGTGGGGAGACTCCTCAT
  GAGGAGATTTTAGCTTATCGAGCTAGTTTGGAGCCATATTCAGATGAGGAATTAGCCCAT
  TTGGTGGATCAAGCAGGCCTTGAGATTCCCCAGTTTAATCGTCGTCGTGCTATGCGTGCC
  TTAGAAATTGCCCATTTTGGTCAGGATTTGGAAAATCAAGAGACCTTGTATGAACCGCTG
  ATTATCTGCTTGGATGATGAACGTAGTCAACTTTATGAGCGTATCAACCACCGAGTGGAT
  TTGATGTTTGAGGCTGGGCTTTTGGATGAGGCCAAGTGGCTGTTTGACCATTCCCCTAAT
  GTACAGGCTGCTAAAGGAATTGGCTATAAGGAACTCTTTCCTTATTTCCGTGGAGAGCAG
  ACCTTGGAGGAAGCTAGTGAGAGTCTCAAACAGGCGACCCGTCGTTTTGCCAAGCGTCAG
  CTGACCTGGTTCCGTAATCGCATGCAGGTCACCTTTTATCAGATTGGAGAATCTGGTGTA
  CAAGACCGCATTTTAAGCCAGATAGAGGAGTTTTTAGATGATTGA
  60
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  840
  885
  

⊟Protein

⊟General

• locus tag: SPCG_RS03295 [old locus tag: SPCG_0628 ]
• symbol: MiaA
• description: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA
• length: 294
• theoretical pI: 4.79178
• theoretical MW: 33288.3
• GRAVY: -0.34898

⊟Function

• reaction:
  EC 2.5.1.75?  ExPASy
  tRNA dimethylallyltransferase Dimethylallyl diphosphate + adenine³⁷ in tRNA = diphosphate + N⁶-dimethylallyladenine³⁷ in tRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA dimethylallyltransferase (TIGR00174; EC 2.5.1.75; HMM-score: 248.2)
  and 10 more
  putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 15.2)
  carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 14.6)
  Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 13)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides endopeptidase La (TIGR00763; EC 3.4.21.53; HMM-score: 12.4)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 12.3)
  Cellular processes Cellular processes Other gas vesicle protein GvpN (TIGR02640; HMM-score: 12.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions cytidylate kinase (TIGR00017; EC 2.7.4.14; HMM-score: 12)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 11.9)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 11.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 10.8)
• TheSEED: see SPCG_0628
• PFAM:
  P-loop_NTPase (CL0023) IPPT; IPP transferase (PF01715; HMM-score: 239.9)
  and 10 more
  IPT; Isopentenyl transferase (PF01745; HMM-score: 32.7)
  AAA_33; AAA domain (PF13671; HMM-score: 19.4)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 18.5)
  AAA_18; AAA domain (PF13238; HMM-score: 16.9)
  AAA_22; AAA domain (PF13401; HMM-score: 14.3)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 13.9)
  AAA_14; AAA domain (PF13173; HMM-score: 13.4)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 12.8)
  TIP49; TIP49 P-loop domain (PF06068; HMM-score: 12.8)
  Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9994
  • Cytoplasmic Membrane Score: 0.0002
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0003
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.271431
  • TAT(Tat/SPI): 0.007706
  • LIPO(Sec/SPII): 0.016296
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000850193 NCBI
• UniProt: see SPCG_0628

⊟Protein sequence

• MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLDIGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTGLYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRALEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHSPNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIGESGVQDRILSQIEEFLDD

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: miaA > hflX > SPCG_RS03305 > rnz > SPCG_RS03315

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0583 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications