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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_0511 [new locus tag: SPD_RS02745 ]
  • pan locus tag?: PNEUPAN001517000
  • symbol: metF
  • pan gene symbol?: metF
  • synonym:
  • product: 5,10-methylenetetrahydrofolate reductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_0511 [new locus tag: SPD_RS02745 ]
  • symbol: metF
  • product: 5,10-methylenetetrahydrofolate reductase
  • replicon: chromosome
  • strand: +
  • coordinates: 521790..522656
  • length: 867
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTCACGCCAAACACCGTCACTCTCATTTGAAGTGTTCCCTCCAACCCCAGCCGTGGGT
    AATGATAACATTATTTCAGCCTTGCAGGATATGCAGGAGTTGGCTCCCCATTTTATCAGT
    GTAACTGCCAGCAATAATAAATTTAATATCAAGGAAACGACGGTTCGTTTGGCTGACTTT
    ATCCAAAATGATTTGGCGATTCCGACCATTGCCCACTTGCCAGCTATTTATCTAACTAAG
    GACAAGGTTGCTGAAACTATTGCTGACTTGGACAAAGTTGGGGTGCAGAAAATCTTGGCT
    CTTCGTGGGGATATTATTCCAGACGTGGAACCACAAAAGGATTTCCGCTATGCAACCGAC
    TTGATTGAGCTTATCAAGGAACGAGCCCCTCACTTTGATATTATTGGAGCTTGCTATCCA
    GAAGGGCATCCAGATTCGCCAAATCAGATTTCAGATATTCAAAATCTTAAGAAGAAAGTG
    GATGCAGGCTGTTCGAGCCTCGTAACTCAGCTCTTCTTTGACAATGAGCGCTTCTATGAT
    TTTCAGGACAAGTGCATCTTGGCTGGGATTGATGTTCCCATTCATGCAGGAATTATGCCA
    ATTCTGAATCGAAATCAGGCTCTCCGACTCTTGAAGACTTGTGAGAATATCCATCTTCCA
    CGCAAATTTAAAGCCATCTTAGACAAGTATGAGCATGACCCTGAGTCGCTCAGAGCAGCA
    GGACTTGCCTATGCAGTGGACCAAATCGTGGACTTGGTAACTCAGGATATTGCCGGTGTG
    CATCTCTATACGATGAATAATGCTGATACAGCAAAATACATCCATCAAGCAACCCATGCC
    TTGTTTAATCACCAGTCTCTAGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    867

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_0511 [new locus tag: SPD_RS02745 ]
  • symbol: MetF
  • description: 5,10-methylenetetrahydrofolate reductase
  • length: 288
  • theoretical pI: 5.73887
  • theoretical MW: 32137.5
  • GRAVY: -0.150347

⊟Function[edit | edit source]

  • reaction:
    EC 1.5.1.20?  ExPASy
    Methylenetetrahydrofolate reductase (NAD(P)H) 5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aspartate family methylenetetrahydrofolate reductase [NAD(P)H] (TIGR00676; EC 1.5.1.20; HMM-score: 334.7)
    and 3 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid methylenetetrahydrofolate reductase (TIGR00677; EC 1.5.1.20; HMM-score: 194.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone dehypoxanthine futalosine cyclase (TIGR03699; HMM-score: 16.7)
    Hypothetical proteins Conserved radical SAM domain protein, CofH subfamily (TIGR00423; HMM-score: 13.9)
  • TheSEED  :
    • Methylenetetrahydrofolate reductase (NAD(P)H) (EC 1.5.1.20)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)
    and 2 more
    Carbohydrates One-carbon Metabolism One-carbon metabolism by tetrahydropterines  5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)
  • PFAM:
    FAD_oxidored (CL0086) MTHFR; Methylenetetrahydrofolate reductase (PF02219; HMM-score: 239.7)
    and 1 more
    no clan defined Chlam_OMP3; Chlamydia cysteine-rich outer membrane protein 3 (PF03503; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010085
    • TAT(Tat/SPI): 0.001836
    • LIPO(Sec/SPII): 0.000937
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSRQTPSLSFEVFPPTPAVGNDNIISALQDMQELAPHFISVTASNNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALRGDIIPDVEPQKDFRYATDLIELIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKVDAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIHLPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDIAGVHLYTMNNADTAKYIHQATHALFNHQSLG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: MtaR* (repression) regulon, CmbR* (activation (repression)) regulon
    MtaR*(TF)important in Methionine metabolism; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 
    CmbR*(TF)important in Cysteine metabolism; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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